Fig 2: Nrn1 reversed the inhibitory effect of miR-194 on neurite growth of Aß1–42-transduced hippocampal neurons. (A) Neurite of Aß1–42-transduced hippocampal neurons were observed under a microscope after they were immunofluorescently stained. (B) Statistical data of the average longest neurite length. * p < 0.05 when compared with the model group at the same time.

Fig 3: miR-194 was upregulated and Nrn1 mRNA was downregulated in hippocampal neurons after being induced by Aß1–42. * p < 0.05 when compared with the blank group.

Fig 4: Nrn1 is the target gene of miR-194. (A) Target Scan predicted the binding sites of Nrn1 and miR-194. (B) Luciferase reporter gene assay was used to verify the targeting relationship between miR-194 and Nrn1. * p < 0.05 when compared with the negative control (NC) + wild-type (Wt) group.

Fig 5: Nrn1 repaired the damage of miR-194 on Aß1–42-transduced hippocampal neuron activity. * p < 0.05 when compared with the model group at the same time.