Fig 1: Effects of repeated administration of CL316243 and/or OEA on gene expression of molecules implicated in mitochondrial programmes (Cox4i1 and Cox4i2) and the BAT-like phenotype (Fgf21 and Prdm16) in eWAT after 6 days of treatment. (A–D) Cox4i1, Cox4i2, Fgf21 and Prdm16 mRNA levels in eWAT. (E) PRDM16 protein levels in eWAT. Histograms represent the mean±s.e.m. (n=8). *P<0.05 versus vehicle-treated rats; #P<0.05 versus CL316243-treated rats; $P<0.05, $$P<0.01 versus OEA-treated rats.
Fig 2: EA increased WAT-browning related proteins in IWAT of HFD + EA group but not in HFD group. SIRT-1 (A, B), PRDM16 (C, D), and p-AMPK (E, F) expression in IWAT obtained from ND-fed, HFD-fed or HFD + EA-fed rats following 14 weeks of treatment. Data were represented as mean ± SD (n = 3). ##P < 0.01 vs. the ND group, *P < 0.05 vs. the HFD + EA group.
Fig 3: EA treatment facilitated the interaction between PPAR? and PRDM-16 and decreased PPAR? and PGC-1a acetylation. IWAT tissues from ND, HFD and HFD + EA groups were immunoprecipitated with acetylated lysine (AcK) antibody and immunoblotted with PPAR? and PGC-1a antibodies. (A) Western blot for Co-IP studies of PPAR? and PRDM16. (B) Acetylation of PPAR? and PGC-1a in white adipose tissue. The ratios of acetylated PPAR? to PPAR? (C) and acetylated PGC-1a to PGC-1a (D). Data were represented as mean ± SD (n = 3). ##P < 0.01 vs. the ND group; **P < 0.01 vs. the HFD + EA group.
Fig 4: The mRNA expression of PGC1a and PRDM16 in back and abdominal fat tissues between the surviving and dead piglets. a-b, The relative mRNA levels of PGC1a and PRDM16 were elevated in both fat pads in surviving piglets compared to the dead piglets. *P < 0.05, n = 10
Fig 5: LINC00589-dominated regulatory networks in breast cancer patients’ tissues.A–D mRNA expression of LINC00589, miR-100, miR-452, DLG5, and PRDM16 of the dissected tissues from 71 patients were measured by qRT-PCR assay. The correlation of LINC00589-miR-100 (A), LINC00589-miR-452 (B), miR-100-DLG5 (C), and miR-452-PRDM16 (D) were analyzed by the Spearman correlation test. E Protein expression of DLG5 and PRDM16 were analyzed by immunohistochemistry (IHC) in tissues from trastuzumab-responding or non-responding patients. Scale bar 50 µm.
Supplier Page from Abcam for Anti-PRDM16 antibody