Fig 1: Immunofluorescence of serial sections through the back skin of adult mice. Sections were stained with Abs against NFAT5 (ab110995, Abcam) and Mmp3 (ab53015 (Abcam) and counterstained with DAPI as indicated. Length of the bars: 100 µm.
Fig 2: Immunofluorescence of sections through the skin of a human fetus. Sections were stained with Abs against NFAT5 (ab110995, Abcam) and Mmp3 (AF513, Novus Biologicals) and counterstained with DAPI as indicated. Length of the bars: 100 µm.
Fig 3: Ablation of NFAT5 changed gene expression in cultivated KCs. (A) Heat map of 168 genes that were changed at least twofold in 129sv Nfat5-/- compared to 129sv WT KCs. (B) Compilation of 121 genes that were expressed in 2-fold more copies in Nfat5-/- KCs. Nine genes encoding TFs are shown in blue and indicated by blue arrows. Five protease genes are boxed and shown in red, four keratin genes are shown in green boxes and by green arrows. The gene coding for the cell cycle inhibitor Cdkn2a is shown in black, genes encoding membrane transporters are shown violet, and the numerous genes encoding Epidermal Differentiation Complex (EDC) proteins, claudins and other ‘cornification` proteins are encircled and presented in orange.
Fig 4: NFAT5 ablation changes the integrity and morphology of murine epidermis. (A, B) Preparations of skin from 129sv WT (A) and 129sv Nfat5-/- mice (B, C). In (C), skin sheets released from tail skin of Nfat5-/- mice are presented at larger magnification. Bar= 100 µM. (D, E) Tape strips from the tails of WT (D) and Nfat5-/- mice (E). One representative strip of more than ten is shown. (F, G) Representative H&E stains of skin sections from tails of WT (F) and Nfat5-/- mice (G). s.c., stratum corneum; s.g., stratum granulosum; s.s., stratum spinosum; s.b., stratum basale.
Fig 5: Proteins that were secreted by isolated tail KCs within 24 h of culture. (A) Proteins that were 2-fold and more secreted by 129sv Nfat5-/- than 129sv WT KCs. (B) Proteins that were at least 2-fold less secreted by Nfat5-/- than WT KCs. Data of two independent experiments each analyzed in triplicates by LC-MS were compiled.
Supplier Page from Abcam for Anti-NFAT5 antibody