Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] ab71286 from Abcam

Product Specs
ItemAnti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab71286
ClonalityMonoclonal
IsotypeIgG1
Antibody TypePrimary
Conjugate/TagUnconjugated
CloneFIS 2F11/2
Quantity100 UG
ApplicationsELISA, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
HostMouse, Nude mouse
Concentration1 mg/ml
ReactivityZebrafish
TargetZebrafish Gut Secretory Cell Epitopes

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Loss of zebrafish pkd1l1 causes biliary defects that have implications for biliary atresia splenic malformationDisease Models & MechanismsOctober 1, 2023Rouknuddin Q. Ali, Anne Meyer-Miner, Marie David-Rachel, Fiona J. H. Lee, Benjamin J. Wilkins, Saul J. Karpen, Brian Ciruna, Anand Ghanekar, Binita M. Kamatharchitecture of the biliary tree, 5 dpf zebrafish larvae were stained with the 2F11 antibody (Abcam, ab71286). Larvae were fixed with 4% paraformaldehyde overnight and then in 100% ice-cold methanol for long-term

(2) Loss of Mtm1 causes cholestatic liver disease in a model of X-linked myotubular myopathyJournal of Clinical InvestigationSeptember 15, 2023Sophie Karolczak, Ashish R. Deshwar, Evangelina Aristegui, Binita M. Kamath, Michael W. Lawlor, Gaia Andreoletti, Jonathan Volpatti, Jillian L. Ellis, Chunyue Yin, James J. Dowlingab155421, 1:200); GFP (Abcam, ab252881, 1:100); Rab11 (Genetex, GTX127328, 1:300); and 2F-11 (Abcam, ab71286, 1:100). Transgenic zebrafish lines. The Tg(Tp1:GFP) line (ZDB-ALT-090625-1) and the Tg(fabp10:GFP-CAAX)

(3) The MRN complex maintains the biliary-derived hepatocytes in liver regeneration through ATR-Chk1 pathwaynpj Regenerative MedicineApril 6, 2023Jingmei Song, Jianlong Ma, Xing Liu, Zhuofu Huang, Lianghui Li, Linke Li, Lingfei Luo, Rui Ni, Jianbo He(1:500, TA180094, Origene), PCNA (1:500, ab29, Abcam), γH2AX (1:500, GTX127342, Gene Tex), 2F11 (1:1000, ab71286, Abcam), GFP (1:1000, ab6658, Abcam), mCherry (1:500, ARG55723, ArigoBio), DsRed2 (1:1000, sc101526,

(4) Regenerative failure of intrahepatic biliary cells in Alagille syndrome rescued by elevated Jagged/Notch/Sox9 signalingProceedings of the National Academy of SciencesDecember 13, 2022Chengjian Zhao, Jonathan Matalonga, Joseph J. Lancman, Lu Liu, Chaoxin Xiao, Shiv Kumar, Keith P. Gates, Jiaye He, Alyssa Graves, Jan Huisken, et al.anti-pan-Cadherin (1:5,000; Sigma), mouse anti-Mdr1 (1:300, sc-71557; Santa Cruz), mouse anti-Anxa4 (1:100, ab71286, aka 2F11; Abcam) (8), chicken anti-GFP (1:300, GFP1010; Aves Labs), goat anti-mCherry (1:500, LS-C204207;

(5) DNA methylation maintenance at the p53 locus initiates biliary-mediated liver regenerationnpj Regenerative MedicineDecember 1, 2022Jianbo He et al.several times with PT (PBS + 1% Triton X-100) and incubated with primary antibodies below: Anxa4 (1:1000; ab71286, Abcam, Cambridge, MA), 5-methyl-cytosine (1:100; ab10805, Abcam, Cambridge, MA), Alcam (1:50; zn5,

(6) Shikonin Inhibits Fin Regeneration in Zebrafish LarvaeCellsOctober 11, 2022Zigang Cao, Chen Guo, Guilan Chen, Jiejun Liu, Huiwen Ni, Fangsheng Liu, Guanghua Xiong, Xinjun Liao, Huiqiang Lublocked with PBTN (4% BSA, 0.02% NaN3, 3% PT) 2 h, followed by the addition of PCNA antibody (1:500, ab71286, Abcam, Cambridge, UK) and left overnight at 4 °C. The primary antibody was recovered on the third day,

(7) Interleukin-10 regulates goblet cell numbers through Notch signaling in the developing zebrafish intestineMucosal immunologyMay 1, 2022Morales RA, Rabahi S, Diaz OE, Salloum Y, Kern BC, Westling M, Luo X, Parigi SM, Monasterio G, Das S, et al.room temperature for more than 2 h. The primary mouse monoclonal 2F11 antibody (1:200; Abcam, Cat. No. ab71286) was diluted in blocking solution and incubated at 4 °C for more than 24 h. Following primary antibody

(8) Intrahepatic cholangiocyte regeneration from an Fgf-dependent extrahepatic progenitor niche in a zebrafish model of Alagille SyndromeHepatology (Baltimore, Md.)March 1, 2022Zhao C, Lancman JJ, Yang Y, Gates KP, Cao D, Barske L, Matalonga J, Pan X, He J, Graves A, et al.anti-Islet1/2 (1:20, 39.4D5, DSHB), rabbit anti-pan-Cadherin (1:5000; Sigma), mouse anti-Anxa4 (1:100, ab71286, aka 2F11, Abcam)(15), rabbit anti-Bhmt (1:200, ab96415, Abcam), mouse anti-Mdr1(1:200, sc-71557, Santa Cruz

(9) Nitazoxanide induced myocardial injury in zebrafish embryos by activating oxidative stress responseJournal of Cellular and Molecular MedicineOctober 1, 2021Fanghua Gong, Tianzhu Shen, Jiangnan Zhang, Xuye Wang, Guoqiang Fan, Xiaofang Che, Zhaopeng Xu, Kun Jia, Yong Huang, Xiaokun Li, et al.pericardial epicardium was peeled off under the microscope with a No. 5 forceps. Anti‐PCNA (1:100, ab71286, Abcam, UK) antibody incubation was performed as described in the previous method. 28 Secondary antibodies

(10) Enteroendocrine cells sense bacterial tryptophan catabolites to activate enteric and vagal neuronal pathwaysCell host & microbeFebruary 10, 2021Ye L, Bae M, Cassilly CD, Jabba SV, Thorpe DW, Martin AM, Lu HY, Wang J, Thompson JD, Lickwar CR, et al.RRID:AB_10013483" Mouse anti-zebrafish gut secretory cell epitopes Monoclonal antibody [FIS 2F11/2],Abcam,ab71286 Mouse anti-zebrafish Zn-12 Monoclonal antibody,ZIRC,AB_10013761 Rabbit anti-serotonin whole Polyclonal

(11) Mutations in RABL3 alter KRAS prenylation and are associated with hereditary pancreatic cancerNature geneticsSeptember 1, 2019Nissim S, Leshchiner I, Mancias JD, Greenblatt MB, Maertens O, Cassa CA, Rosenfeld JA, Cox AG, Hedgepeth J, Wucherpfennig JI, et al.heat-induced antigen retrieval. Cholangiocarcinoma was visualized using a primary antibody (2F11; Abcam, ab71286) and processed using an HRP/DAB (ABC) detection kit (Abcam, ab64264). Zebrafish RNA-seq. RNA was extracted

(12) Zebrafish hhex-null mutant develops an intrahepatic intestinal tube due to de-repression of cdx1b and pdx1Journal of Molecular Cell BiologyJune 1, 2019Ce Gao, Weidong Huang, Yuqi Gao, Li Jan Lo, Lingfei Luo, Honghui Huang, Jun Chen, Jinrong Peng(sc-8656-R, 1:600), PCNA antibody from Sigma (P8825, 1:1000), and 2F11 monoclonal antibody from Abcam (ab71286, 1:500). Alexa Fluor 647-labeled secondary antibody was used for visualization. RNA and protein analysis RNA

(13) Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonizationPLoS pathogensMarch 1, 2019Murdoch CC, Espenschied ST, Matty MA, Mueller O, Tobin DM, Rawls JF.buffer overnight at 4˚C with agitation (mouse anti 4E8, Abcam, ab73643, 1:200; mouse anti 2F11, Abcam, ab71286, 1:200; rabbit anti DsRed, Clontech, 632496, 1:200; chicken anti GFP, AVES, GFP-10x0, 1:200). Sections

(14) In vivo imaging of emerging endocrine cells reveals a requirement for PI3K-regulated motility in pancreatic islet morphogenesisDevelopment (Cambridge, England)February 8, 2018Freudenblum J, Iglesias JA, Hermann M, Walsen T, Wilfinger A, Meyer D, Kimmel RA.anti-GFP (Roche, 11814460001, 1:100), mouse anti-glucagon (Sigma, G2654, 1:100), mouse anti-2F11 (Abcam, ab71286, 1:100), rabbit anti-somatostatin (Dako, A0566, 1:200), guinea pig anti-insulin (Dako, A0564, 1:200),

(15) Activation of the LKB1‑SIK1 signaling pathway inhibits the TGF‑β‑mediated epithelial‑mesenchymal transition and apoptosis resistance of ovarian carcinoma cellsMolecular Medicine ReportsDecember 8, 2017Bo Hong, Jianmei Zhang, Wenlan Yangab187109), Twist-related protein 1 (cat. no. ab50887), zinc finger E-box-binding homeobox 1 (ZEB1; cat. no. ab71286), E-cadherin (cat. no. ab76319), vimentin (VIM; cat. no. ab137321), VEGF (cat. no. ab27278), angiotensin-1

(16) A mouse model replicating hippocampal sparing cranial irradiation in humans: A tool for identifying new strategies to limit neurocognitive declineScientific ReportsNovember 1, 2015Wolfgang A. Tomé, Şölen Gökhan, N. Patrik Brodin, Maria E. Gulinello, John Heard, Mark F. Mehler, Chandan Guha

(17) The Role of ARF6 in Biliary AtresiaPLoS ONESeptember 17, 2015Mylarappa Ningappa, Juhoon So, Joseph Glessner, Chethan Ashokkumar, Sarangarajan Ranganathan, Jun Min, Brandon W. Higgs, Qing Sun, Kimberly Haberman, Lori Schmitt, Silvia Vilarinho, Pramod K. Mistry, Gerard Vockley, Anil Dhawan, George K. Gittes, Hakon Hakonarson, Ronald Jaffe, Shankar Subramaniam, Donghun Shin, Rakesh Sindhi

(18) Fate mapping ofptf1a-expressing cells during pancreatic organogenesis and regeneration in zebrafishDevelopmental DynamicsJune 1, 2015Yue J. Wang, Joon T. Park, Michael J. Parsons, Steven D. Leachanti-Somatostatin (Dako, A0566), 1:500. Rabbit anti-DsRed (Clontech, 642496), 1:100. Mouse anti-2F11 (Abcam, ab71286), 1:200. Primary antibodies were incubated at 4°C overnight. After washing with PBST, samples were incubated

(19) Real-Time Whole-Body Visualization of Chikungunya Virus Infection and Host Interferon Response in ZebrafishPLoS PathogensSeptember 5, 2013Nuno Palha et al.

(20) The bHLH transcription factor Ascl1a is essential for the specification of the intestinal secretory cells and mediates Notch signaling in the zebrafish intestineDevelopmental biologyApril 15, 2013Lydie C Flasse, David G Stern, Justine L Pirson, Isabelle Manfroid, Bernard Peers, Marianne L Voz

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Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] from Abcam

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Anti-Zebrafish Gut Secretory Cell Epitopes antibody [FIS 2F11/2] from Abcam

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