Fig 1: ICLite deconvolution of BAL sequencing improves GO term linkage(A) Correlogram of gene module to cell cluster interactions using the ICLite algorithm. Spearman correlation of module scoring with immune cell log ratio was used construct a correlogram of BAL gene modules (x axis) versus cell lineages (y axis). Only associations with false discovery rate (FDR) corrected p < 0.05 are illustrated.(B) Barplot of -log10(p values) for GO term enrichment of ICLite modules 13 and 15, which correlate with CD206+ CD11b+ CCR4+ macrophages. Plotting of patient module score versus immune cell log ratio is adjacent, with Spearman’s rho and p value of comparison as indicated in the figure. Red hashed line indicates linear regression model for data.(C) Barplot of -log10(p values) for GO term enrichment of ICLite modules 10 and 11, which correlate with FceRI+ CD127+ CCR4- innate cells and CD206+ FceRI+ macrophages, respectively. Plotting of respective patient module score versus immune cell log ratio is adjacent, with Spearman’s rho and p value of comparison as indicated in the figure. Red hashed line indicates linear regression model for data.(D) Barplot of -log10(p values) for GO term enrichment of ICLite modules 1 and 20, which correlate with CD4 and CD8 EMs, CMs, and TRMs. Plotting of respective patient module score versus CD4 TRM log ratio is adjacent, with Spearman’s rho and p value of comparison as indicated in the figure. Red hashed line indicates linear regression model for data.