Fig 2: ßENaC overexpression-induced reduction of bronchiolar epithelial SPHK2 expression associated with mucus obstruction. a-c Representative confocal images of SPHK2 immunofluorescence (red) in control (a) and ßENaC mouse lungs, unobstructed (b) vs. obstructed airway (c). d A negative staining control shows no binding of AF594-conjugated anti-rabbit IgG (red). The insets are magnification of boxed areas. Blue is DAPI. Scale bars are in micrometres. e Box plot represents MFI values of SPHK2 expression measured from individual ßENaC mice in unobstructed (red) versus mucus obstructed (green) epithelia (Mann-Whitney, p < 0.01). f Dot plot showing the same data paired for each ßENaC mouse (paired t-test, p < 0.05). Each dot in E and F represent the average of 10 bronchioles