Fig 2: Seasonal immunolocalization of OTR and OT in the scented gland of muskrat. Black arrows indicate the three cell types: glandular cells, epithelial cells and interstitial cells. The first (a,d) and second (b,c,e,f) columns represent staining in the breeding season. The third (g,j) and fourth (h,i,k,l) columns represent staining in the non-breeding season. (a–c, g–i) Immunolocalization of OTR in scented glands. (d–f, j–l) Immunolocalization of OT in scented glands. (m,n) Negative control, sections were treated with normal rabbit serum instead of primary antisera, represent the breeding season and non-breeding season, respectively. (o,p) The mammary gland and uterus of female muskrats were used as positive control for OTR and OT, respectively. GC, glandular cell; EC, epithelial cell; IC, interstitial cell; ED, excretory duct. Scale bars = 50 µm (a,d,g,j); 20 µm (b,c,e,f,h,i,k,l,m,n,o,p).