Western blot was performed using anti-Cardiac Troponin I Monoclonal Antibody (C5) (Product # MA1-20118). A 24 kDa band corresponding to Cardiac Troponin I was observed in Rat Heart, a 21 kDa band corresponding to Cardiac Troponin I was observed in Mouse Heart and a 25 kDa band corresponding to the light chain of Mouse IgG was observed in Mouse Heart. Tissue extracts (50 µg lysate) of Mouse Heart (Lane 1), Rat Brain (Lane 2), Rat Liver (Lane 3) and Rat Heart (Lane 4) were electrophoresed using Novex™ 16% Tris-Glycine Gel (Product # XP00160BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).