Fluorescent confocal image of SY5Y cells using 042166. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 042166 (1:200, 2h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10ug/ml, 5 min). Note the highly specific localization of the phospho-Sox2 immunosignal mainly to the nucleus.