Fig 1: Chorion vessel analysis. Representative fluorescent microscopic images of chorions from (a) WT or (b) Tks4-KO embryos where endothelial cells were visualized by CD34 immunofluorescent labeling (green), scale bar: 100 µm. Quantitative analysis of vessel parameters in 3 WT and 3 Tks4-KO individuals: (c) vessel density, (d) average width of vessels, (e) total length of all vessels in a 865 µm × 650 µm microscopic field. Error bars correspond to SEM and asterisks (*) indicate statistically significant differences with Student’s t-test, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p < 0.05$$\end{document}p<0.05.