Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) 9854S from Cell Signaling Technology

Product Specs
ItemVimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9854S
Quantity100 µl
ApplicationsIHC-P, IF-IC, FC-FP
ReactivityH, M, R, Hm, Mk
TargetVimentin
HostRabbit
Conjugate/TagAlexa Fluor ® 488
ClonalityMonoclonal
NCBI Full Gene Namevimentin
Research AreaCytoskeletal Signaling
IsotypeIgG
CloneD21H3

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Interleukin 36 receptor-inducible matrix metalloproteinase 13 mediates intestinal fibrosisFrontiers in ImmunologyMay 3, 2023Kristina Koop, Karin Enderle, Miriam Hillmann, Laura Ruspeckhofer, Michael Vieth, Gregor Sturm, Zlatko Trajanoski, Anja A. Kühl, Raja Atreya, Moritz Leppkes, et al.anti-human MMP13 (Abcam, #ab39012), A488-labelled rat anti-Vimentin (Cell Signaling Technologies, #9854S), rat anti-human Pdpn (Invitrogen, #13-5381-82), A555-labelled mouse anti-αSMA (Thermo Fisher Scientific,

(2) A gut-brain axis mediates sodium appetite via gastrointestinal peptide regulation on a medulla-hypothalamic circuitScience advancesFebruary 15, 2023Liu Y, Wei JA, Luo Z, Cui J, Luo Y, Mak SOK, Wang S, Zhang F, Yang Y, So KF, et al.AB1541), vimentin XP rabbit monoclonal antibody (Alexa Fluor 48 conjugate) (1:400, Cell Signaling, 9854), guinea pig anti–c-Fos (1:500, Synaptic Systems, 226005), rabbit anti-MC4R (1:1000, Abcam, ab24233),

(3) An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancerCell reports. MedicineFebruary 15, 2022Johnson BE, Creason AL, Stommel JM, Keck JM, Parmar S, Betts CB, Blucher A, Boniface C, Bucher E, Burlingame E, et al.,Cell Signaling Technology,Cat# 3465; RRID: AB_10695860 Rabbit monoclonal anti-Vimentin,Cell Signaling Technology,Cat# 9854; RRID: AB_10829352 Rabbit monoclonal anti-Collagen I,Abcam,Cat# ab215969 Mouse monoclonal anti-Collagen

(4) Loss of hexokinase 1 sensitizes ovarian cancer to high-dose metforminCancer & metabolismDecember 11, 2021Šimčíková D, Gardáš D, Hložková K, Hruda M, Žáček P, Rob L, Heneberg P.cultured cells was tested by staining with vimentin-Alexa488 (Cell Signaling Technology, Danvers, MA, #9854), pan-keratin-PE (Cell Signaling Technology, #5075), and smooth muscle actin-Alexa488 (eBioscience,

(5) Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platformsCell Reports MethodsAugust 23, 2021Erik A. Burlingame, Jennifer Eng, Guillaume Thibault, Koei Chin, Joe W. Gray, Young Hwan ChangEPR3094),Abcam,Cat#ab218582 Rabbit anti-Vimentin (Vim) (clone D21H3),Cell Signaling Technology,Cat#9854 Rabbit anti-Collagen I (ColI) (clone EPR7785),Abcam,Cat#ab215969 Mouse anti-Collagen IV (ColIV) (clone

(6) Irradiation Accelerates Plaque Formation and Cellular Senescence in Flow-Altered Carotid Arteries of Apolipoprotein E Knock-Out MiceJournal of the American Heart AssociationJuly 20, 2021Yamamoto Y, Minami M, Yoshida K, Nagata M, Miyata T, Yang T, Takayama N, Suzuki K, Okawa M, Yamada K, et al.monoclonal, 1:200, Cell Signaling Technology, Danvers, MA), antivimentin (Alexa Fluor 488 conjugate, 9854, rabbit monoclonal, 1:200, Cell Signaling Technology), anti‐Iba‐1 (013‐27691, rabbit polyclonal, 1:1000,

(7) JOSD1 inhibits mitochondrial apoptotic signalling to drive acquired chemoresistance in gynaecological cancer by stabilizing MCL1Cell Death & DifferentiationJanuary 1, 2020Xiaowei Wu, Qingyu Luo, Pengfei Zhao, Wan Chang, Yating Wang, Tong Shu, Fang Ding, Bin Li, Zhihua LiuAlexa Fluor 488 (#4412, Cell Signaling Technology) or anti-rabbit IgG conjugated to Alexa Fluor 594 (#9854, Cell Signaling Technology), and nuclei were stained with Hoechst 33342 (Thermo Scientiﬁc) for 15 min

(8) Targeting cardiac fibrosis with engineered T cellsNatureSeptember 19, 2019Haig Aghajanian et al.available in PMC 2020 March 11. Aghajanian et al. Page 6 Author Manuscript Vimentin antibody (#9854) anti-F4/90 antibody (#70076), anti-CD19 antibody (#90176), and anti-CD8 antibody (#98941) were obtained

(9) Hepatitis B virus infection is not associated with fatty liver disease: Evidence from a cohort study and functional analysisMolecular Medicine ReportsNovember 2, 2018Bingqian Wang, Wenna Li, Hezhi Fang, Huaibin Zhouwere washed with PBS and incubated with an Alexa Fluor® 488-labeled immunoglobulin secondary antibody (9854; 1:250; Cell Signaling Technology, Inc.) for 45 min at room temperature. A final incubation was performed

(10) Quercetin is able to alleviate TGF‑β‑induced fibrosis in renal tubular epithelial cells by suppressing miR‑21Experimental and Therapeutic MedicineJuly 20, 2018Yaochen Cao, Jialin Hu, Jianying Sui, Limei Jiang, Yakun Cong, Guoqing Renthree times with PBS, cells were incubated with Alexa Fluor® 488‑conjugated rabbit mAb (1:800; cat. no. 9854; Cell 2444 EXPERIMENTAL AND THERAPEUTIC MEDICINE 16: 2442-2448, 2018 Signaling Technology, Inc.)

(11) SUV420H2 is an epigenetic regulator of epithelial/mesenchymal states in pancreatic cancerThe Journal of cell biologyFebruary 5, 2018Viotti M, Wilson C, McCleland M, Koeppen H, Haley B, Jhunjhunwala S, Klijn C, Modrusan Z, Arnott D, Classon M, et al.Molecular Probes), and the following fluorescently conjugated monoclonal antibodies: rabbit Vim_488 (9854; Cell Signaling Technology), mouse E-Cadherin_555 (560064; BD), and mouse EPCAM_647 (324212; BioLegend).

(12) Attenuated PDGF signaling drives alveolar and microvascular defects in neonatal chronic lung diseaseEMBO molecular medicineNovember 1, 2017Oak P, Pritzke T, Thiel I, Koschlig M, Mous DS, Windhorst A, Jain N, Eickelberg O, Foerster K, Schulze A, et al.#562759), CD90 (BD Pharmingen #561974), PDGF‐Rα (eBiosciences #17‐1401), Vimentin (Cell Signaling #9854), and α‐SMA (R&D systems #IC1420P). Mechanical stretch Myofibroblasts were seeded on flexible‐bottomed

(13) Proteomic analysis of ovarian cancer cells during epithelial-mesenchymal transition (EMT) induced by epidermal growth factor (EGF) reveals mechanisms of cell cycle controlJournal of proteomicsJanuary 16, 2017Grassi ML, Palma CS, Thomé CH, Lanfredi GP, Poersch A, Faça VM.

(14) A protocol for the culture and isolation of murine synovial fibroblastsBiomedical ReportsAugust 1, 2016Jinjun Zhao, Qingqing Ouyang, Ziyou Hu, Qin Huang, Jing Wu, Ran Wang, Min Yang(Franklin Lakes, NJ, USA), vimentin rabbit monoclonal antibody (Alexa Flour 488 Conjugate; cat. no. 9854) was obtained from Cell Signaling Technology (Beverly, MA, USA). Preparation of reagents Culture medium

(15) Leukemia Inhibitory Factor Enhances Endogenous Cardiomyocyte Regeneration after Myocardial InfarctionPloS oneJanuary 1, 2016Kanda M, Nagai T, Takahashi T, Liu ML, Kondou N, Naito AT, Akazawa H, Sashida G, Iwama A, Komuro I, et al.

(16) A detailed protocol for a rapid analysis of testicular cell populations using flow cytometryAndrologySeptember 1, 2015E. Rotgers, S. Cisneros‐Montalvo, K. Jahnukainen, J. Sandholm, J. Toppari, M. Nurmiofrom the same litter and the n refers to litter. Rabbit anti‐vimentin conjugated to Alexa488 (cat. # 9854; Cell Signaling Technologies) was used at a concentration of 1/50 to detect vimentin‐positive cells

(17) BMPRIA Mediated Signaling Is Essential for Temporomandibular Joint Development in MicePLoS ONEAugust 5, 2014Shuping Gu, Weijie Wu, Chao Liu, Ling Yang, Cheng Sun, Wenduo Ye, Xihai Li, Jianquan Chen, Fanxin Long, YiPing Chenand then incubated with primary antibodies against BMPRIA (Abcam; ab38560), Lubricin (Santa Cruz; sc-9854), pSmad1/5, pJNK, pERK, and p-p38 (from Cell Signaling; #9516, #9255, #4370, and #9211), respectively,

(18) Signal transduction in primary human T lymphocytes in altered gravity – results of the MASER-12 suborbital space flight missionCell Communication and SignalingDecember 1, 2013Svantje Tauber et al.(Cell Signaling, 9683), 1:400; Phospho-Histone H3 (Cell Signaling 9708), 1:40; Vimentin (Cell Signaling, 9854), 1:320; Mouse anti-LAT (pY171) (BD Bioscience, 558519), 1:8; Mouse (MOPC-21) mAb IgG1 Isotype Control

(19) Hedgehog Signaling Alters Reliance on EGF Receptor Signaling and Mediates Anti-EGFR Therapeutic Resistance in Head and Neck CancerCancer ResearchJune 1, 2013Stephen B. Keysar et al.shaking. Cells were incubated with E-cadherin (Cell Signaling 4295) and/or Vimentin (Cell Signaling 9854) for 1.5h at RT at 1:50 and 1:200 dilutions, respectively. Alternatively, cells were incubated with

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Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate)

Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) from Cell Signaling Technology

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Cell Signaling Technology

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Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) from Cell Signaling Technology

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