GFP Antibody NB600-303 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemGFP Antibody
CompanyNovus Biologicals, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberNB600-303
ImmunogenThe immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
Conjugate/TagUnconjugated
ApplicationsWestern Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Electron Microscopy, Chromatin Immunoprecipitation (ChIP)
ReactivityNon-species specific
Format0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Quantity0.05 ml
HostRabbit
TargetGFP
Brand/TrademarkNovus Biologicals, a Bio-Techne Brand
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaEpitope Tags
IsotypeIgG
Antibody TypePrimary Antibodies
StorageStore at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Unconjugated

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Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: http://www.novusbio.com

(1) Ca²⁺-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repairNature communicationsApril 6, 2022Niekamp P, Scharte F, Sokoya T, Vittadello L, Kim Y, Deng Y, Südhoff E, Hilderink A, Imlau M, Clarke CJ, et al.[R960-25] (Invitrogen, r96025; IF 1:400; IB 1:1000); rabbit polyclonal anti-GFP (Novus Biologicals, NB600-303; IF 1:250); HRP-conjugated goat anti-mouse IgG (Thermo Fisher Scientific, 31430; IB 1:5000); HRP-conjugated

(2) CLIP-170S is a microtubule +TIP variant that confers resistance to taxanes by impairing drug-target engagementDevelopmental cellDecember 6, 2021Thakkar PV, Kita K, Castillo UD, Galletti G, Madhukar N, Navarro EV, Barasoain I, Goodson HV, Sackett D, Díaz JF, et al.clone),Millipore-Sigma,"Cat No: T6199-100UL, RRID:AB_477583" Rabbit anti-GFP antibody,Novus Biological,"Cat No: NB600-303, RRID:AB_10001300" Rat anti-α-tubulin monoclonal antibody (YL1/2 clone),Bio-rad,"Cat No: MCA77G, RRID:AB_325003" EB-1

(3) Hair Regeneration Potential of Human Dermal Sheath Cells Cultured Under Physiological OxygenTissue engineering. Part ANovember 1, 2020Kanayama K, Takada H, Saito N, Kato H, Kinoshita K, Shirado T, Mashiko T, Asahi R, Mori M, Tashiro K, et al.

(4) Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5^-CD44⁺ cellsNature communicationsJanuary 3, 2020Fu DJ, Wang L, Chouairi FK, Rose IM, Abetov DA, Miller AD, Yamulla RJ, Schimenti JC, Flesken-Nikitin A, Nikitin AY.buffer (pH 6.0) for 10 min. The primary antibodies against GFP (NOVUS biological, Littleton, CO, USA; NB600-303; 1:8000), CD44 (Santa Cruz Biotechnologies, Dallas, TX, USA; sc-18849; 1:1000), Mucin5AC (Abcam, Cambridge,

(5) ALG-1 Influences Accurate mRNA Splicing Patterns in the Caenorhabditis elegans Intestine and Body Muscle Tissues by Modulating Splicing Factor ActivitiesGeneticsJuly 1, 2019Kotagama K, Schorr AL, Steber HS, Mangone M.described below) followed by a western blot. For the western blot we used a primary anti-GFP antibody (NB600-303; Novus) (1:2000) miRNAs and Alternative Splicing in C. elegans 933 and a ﬂuorescent secondary

(6) Ras homolog family member A/Rho-associated protein kinase 1 signaling modulates lineage commitment of mesenchymal stem cells in asthmatic patients through lymphoid enhancer-binding factor 1The Journal of allergy and clinical immunologyApril 1, 2019Ke X, Do DC, Li C, Zhao Y, Kollarik M, Fu Q, Wan M, Gao P.Technology Epi-CAM,G8.8,Rat,IHC (1:100),ThermoFisher EYFP,GF28R,Mouse,IHC (1:100),ThermoFisher ,NB600–303,Rabbit,IHC (1:100),Novus Biologicals Lef1,C12A5,Rabbit,IHC (1:200),Cell Signaling Technology LepR,BAF497,Goat,FC

(7) Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetryMolecular biology of the cellDecember 15, 2018Bärenz F, Kschonsak YT, Meyer A, Jafarpour A, Lorenz H, Hoffmann I.obtained from Sigma, goat anti-γ-tubulin C-20 (1:50, sc-7396) from Santa Cruz, rabbit anti-GFP (1:500; NB600-303) from Novus, rabbit anti-pericentrin (1:3000; ab4448) and rabbit anti-Cep170 (1:500; ab72505) from Abcam,

(8) Highly Multiplexed CRISPRi Repression of Respiratory Functions Enhances Mitochondrial Localized Ethyl Acetate Biosynthesis in Kluyveromyces marxianusACS synthetic biologyNovember 16, 2018Löbs AK, Schwartz C, Thorwall S, Wheeldon I.

(9) HGF/R-spondin1 rescues liver dysfunction through the induction of Lgr5⁺ liver stem cellsNature communicationsOctober 27, 2017Lin Y, Fang ZP, Liu HJ, Wang LJ, Cheng Z, Tang N, Li T, Liu T, Han HX, Cao G, et al.were blocked with 1% bovine serum albumin (Sigma) and incubated with a primary antibody to GFP (1:200, NB600-303, Novus Biologicals) or Lgr5 (1:100, ab75850, Abcam) at 37 °C for 1 h. After washing extensively, the

(10) Telomere DNA damage signaling regulates cancer stem cell evolution, epithelial mesenchymal transition, and metastasisOncotargetOctober 6, 2017Lagunas AM, Wu J, Crowe DL.or dissociated for flow cytometry. Antibodies TRF2 Santa Cruz Biotechnology SC-9143, α-actin Novus NB600-303, 53BP1 Novus NB100-304, pATM Santa Cruz Biotechnology SC-47739, pChk2 Abcam AB85743, p53 BD Biosciences

(11) Modulation of estrogen related receptor alpha activity by the kinesin KIF17OncotargetAugust 1, 2017Seneviratne APB, Turan Z, Hermant A, Lecine P, Smith WO, Borg JP, Jaulin F, Kreitzer G.

(12) The ErbB3 receptor tyrosine kinase negatively regulates Paneth cells by PI3K-dependent suppression of Atoh1Cell death and differentiationMay 1, 2017Almohazey D, Lo YH, Vossler CV, Simmons AJ, Hsieh JJ, Bucar EB, Schumacher MA, Hamilton KE, Lau KS, Shroyer NF, et al.with Protein G Dynabeads (Invitrogen, #100-03D) pre-bound to 2 μg anti-GFP (Novus, Littleton, CO, USA, NB600-303) at 4 °C overnight, washed five times in 50 mM HEPES pH 8.0 with 1% Nonidet P-40, 1% sodium deoxycholate,

(13) Diverting CERT-mediated ceramide transport to mitochondria triggers Bax-dependent apoptosisJournal of cell scienceJanuary 15, 2017Jain A, Beutel O, Ebell K, Korneev S, Holthuis JC.

(14) Transcriptional Regulation by ATOH1 and its Target SPDEF in the IntestineCellular and molecular gastroenterology and hepatologyJanuary 1, 2017Lo YH, Chung E, Li Z, Wan YW, Mahe MM, Chen MS, Noah TK, Bell KN, Yalamanchili HK, Klisch TJ, et al.transfected cells were used in chromatin immunoprecipitation (ChIP) experiments with antibodies against GFP (NB600-303; Novus, Littleton, CO), Flag M2 (F1804; Sigma), H3k27Ac (ab4729; Abcam, Cambridge, MA), or H3K27me3

(15) Fbxo28 promotes mitotic progression and regulates topoisomerase IIα-dependent DNA decatenationCell cycle (Georgetown, Tex.)December 16, 2016Kratz AS, Richter KT, Schlosser YT, Schmitt M, Shumilov A, Delecluse HJ, Hoffmann I.

(16) Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stressJournal of molecular and cellular cardiologyAugust 1, 2016Magarin M, Pohl T, Lill A, Schulz H, Blaschke F, Heuser A, Thierfelder L, Donath S, Drenckhahn JD.

(17) Cep78 is a new centriolar protein involved in Plk4-induced centriole overduplicationJournal of cell scienceJuly 15, 2016Brunk K, Zhu M, Bärenz F, Kratz AS, Haselmann-Weiss U, Antony C, Hoffmann I.

(18) pH Regulation by NHX-Type Antiporters Is Required for Receptor-Mediated Protein Trafficking to the Vacuole in ArabidopsisThe Plant cellApril 1, 2015Reguera M, Bassil E, Tajima H, Wimmer M, Chanoca A, Otegui MS, Paris N, Blumwald E.

(19) Plk4-dependent phosphorylation of STIL is required for centriole duplicationBiology openFebruary 20, 2015Kratz AS, Bärenz F, Richter KT, Hoffmann I.Santa Cruz Biotechnology. Mouse anti-actin (JLA20) was from Calbiochem, mouse anti-His from Qiagen, rabbit anti-GFP (NB600-303) from Novus, rabbit anti-CP110 (A301-343A) and rabbit anti-STIL (A302-442A) for western blotting from

(20) Stress-induced chloroplast degradation in Arabidopsis is regulated via a process independent of autophagy and senescence-associated vacuolesThe Plant cellDecember 1, 2014Wang S, Blumwald E.

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11/8/2021GFP Antibody for IHC-P SARITHA D'SOUZAThis antibody was used to detected GFP positive human iPSC cells in a teratoma section. The antibody was easy to use and effectively detected GFP positive cells in the paraffin section.

GFP Antibody

GFP Antibody

GFP Antibody

GFP Antibody

GFP Antibody

GFP Antibody from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Novus Biologicals, a Bio-Techne Brand

Citations (52)

Reviews

GFP Antibody from Novus Biologicals, a Bio-Techne Brand

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