Western Blot of CEACAM1/CEACAM5 in whole cell lysate (5 µg) from UACC-257, SK-MEL-28, Hep-G2, SUP-M2, HeLa, HEK293T, Jurkat, RAW 264.7, and TCMK-1 cells. Samples were treated with NETN lysis buffer, incubated with CEACAM1/CEACAM5 monoclonal antibody (Product # MA5-44260) at a dilution of 1:1000, followed by HRP-conjugated goat anti-rabbit IgG (exposure time of 1 seconds). Lower Panel: Rabbit anti-Actin.