Anti-GAPDH antibody - Loading Control (HRP) ab9385 from Abcam

Product Specs
ItemAnti-GAPDH antibody - Loading Control (HRP)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab9385
Antibody TypePrimary
ApplicationsWestern blot
IsotypeIgG
ClonalityPolyclonal
HostRabbit
Quantity100 UL
Conjugate/TagHRP
Concentration1 mg/ml
Gene NameGAPDH
ReactivityHuman
TargetGAPDH

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(1) Targeting neuronal lysosomal dysfunction caused by β-glucocerebrosidase deficiency with an enzyme-based brain shuttle constructNature communicationsApril 12, 2023Gehrlein A, Udayar V, Anastasi N, Morella ML, Ruf I, Brugger D, von der Mark S, Thoma R, Rufer A, Heer D, et al.anti-hIgG (Fc-specific) (Jackson ImmunoResearch, #109-116-170), HRP-conjugated rb pAb to GAPDH (Abcam, #ab9385). Antibody dilution is indicated in respective methods sections. Cell cultures Human neuroblastoma

(2) Lamin A/C phosphorylation at serine 22 is a conserved heat shock response to regulate nuclear adaptation during stressJournal of Cell ScienceFebruary 15, 2023Laura Virtanen, Emilia Holm, Mona Halme, Gun West, Fanny Lindholm, Josef Gullmets, Juho Irjala, Tiina Heliö, Artur Padzik, Annika Meinander, et al.rat monoclonal anti-HSC70/HSP73 (1:1000, 1B5, Enzo Life Sciences), HRP-conjugated anti-GAPDH (1:5000, ab9385, Abcam) and mouse monoclonal anti-actin (1:1000, AC-40, Sigma-Aldrich). The secondary antibodies were

(3) Cerivastatin Synergizes with Trametinib and Enhances Its Efficacy in the Therapy of Uveal MelanomaCancers (Basel)January 31, 2023Adriana Agnese Amaro, Rosaria Gangemi, Laura Emionite, Patrizio Castagnola, Gilberto Filaci, Martine J. Jager, Enrica Teresa Tanda, Francesco Spagnolo, Matteo Mascherini, Ulrich Pfeffer, et al.Sigma-Aldrich), anti-β-tubulin (HRP) rabbit polyclonal (ab21058) and anti-GAPDH rabbit polyclonal antibodies (ab9385) from Abcam (Cambridge, MA, USA). Antibody binding was revealed by ECL Prime (RPN2232, GE Healthcare,

(4) Pro-inflammatory mediators released by activated monocytes promote aortic valve fibrocalcific activityMolecular MedicineDecember 1, 2022Peijian Zhang, Erlinda The, Zichao Luo, Yufeng Zhai, Qingzhou Yao, Lihua Ao, David A. Fullerton, Dingli Xu, Xianzhong MengAntibodies against Alkaline Phosphatase (ALP) (ab108337), type IV collagen (collagen IV) (ab6586) and GAPDH (ab9385) were obtained from Abcam (Cambridge, MA). Antibody against bone morphogenetic protein 2 (BMP2) (XP-5111)

(5) Self-assembling short immunostimulatory duplex RNAs with broad-spectrum antiviral activityMolecular Therapy - Nucleic AcidsSeptember 13, 2022Longlong Si, Haiqing Bai, Crystal Yuri Oh, Amanda Jiang, Fan Hong, Tian Zhang, Yongxin Ye, Tristan X. Jordan, James Logue, Marisa McGrath, et al.study were anti-IRF3 (Abcam, ab68481), anti-IRF3 (Phospho S396) (Abcam, ab138449), anti-GAPDH (Abcam, ab9385), and goat anti-rabbit IgG H&L (HRP) (Abcam, ab205718). Cells were harvested and lysed in radio-immunoprecipitation assay

(6) Self-assembling short immunostimulatory duplex RNAs with broad spectrum antiviral activitybioRxivJuly 18, 2022Longlong Si et al.study were anti-IRF3 (Abcam, ab68481), anti-IRF3 (Phospho S396) (Abcam, ab138449), anti-GAPDH (Abcam, ab9385), and Goat anti-Rabbit IgG H&L (HRP) (Abcam, ab205718). Cells were harvested and lysed in RIPA buffer

(7) Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3pMolecular Medicine ReportsJanuary 14, 2022Mingliang Zhang, Songbo Ma, Xuzhao Li, Henghai Yu, Yizheng Tan, Jun He, Xiaoping Wei, Junming Maantibodies (anti-USP22; 1:2,000; cat. no. LS-C102769; LifeSpan BioSciences, Inc.; anti-GAPDH; 1:1,000; ab9385; Abcam) at 4°C overnight and then incubated with a HRP-conjugated secondary antibody (1:10,000; ab6721;

(8) Morphine promotes the malignant biological behavior of non-small cell lung cancer cells through the MOR/Src/mTOR pathwayCancer cell internationalNovember 25, 2021Liu X, Yang J, Yang C, Huang X, Han M, Kang F, Li J.Proteintech, China), anti-mTOR (66888–1-Ig, Proteintech, China), anti-MOR (ab10275, Abcam, UK); anti-GAPDH (ab9385, Abcam, UK); Matrigel (Corning, USA), anti-BCL2 (60178–1-Ig, Proteintech, China); anti-BAX (60267–1-Ig,

(9) Ononin alleviates H₂O₂‑induced cardiomyocyte apoptosis and improves cardiac function by activating the AMPK/mTOR/autophagy pathwayExperimental and Therapeutic MedicineSeptember 16, 2021Rongrong Pan, Qin Zhuang, Jiangtin Wang3869), Bax (1:1,000; Cell Signaling Technology, Inc., cat. no. 2772), GAPDH (1:10,000, Abcam, cat. no. ab9385), p62 (1:1,000; Cell Signaling Technology, Inc., cat. no. 5114), LC3-I (1:1,000; Cell Signaling Technology,

(10) MicroRNA-125b inhibits the proliferation of vascular smooth muscle cells induced by platelet-derived growth factor BBExperimental and therapeutic medicineAugust 1, 2021Wang X, Chen S, Gao Y, Yu C, Nie Z, Lu R, Sun Y, Guan Z.(dilution, 1:1,000; cat. no. 21220‑1‑AP; ProteinTech Group, Inc.) and GAPDH (dilution, 1:10,000; cat. no. ab9385; Abcam) at 4˚C overnight. Following incubating with the primary antibody, membranes were incubated with secondary

(11) The fidelity of DNA replication, particularly on GC-rich templates, is reduced by defects of the Fe–S cluster in DNA polymerase δNucleic Acids ResearchJune 4, 2021Denis A Kiktev et al.Scientific) membrane. Membranes were probed with anti-HA antibody (ab9110, Abcam) or an anti-GAPDH antibody (ab9385, Abcam). Multi-subunit DNA polymerase δ purification was adapted from (37–40). Briefly, overexpression

(12) Dynamics of SAS-I mediated H4 K16 acetylation during DNA replication in yeastPLOS ONEMay 20, 2021Mark Boltengagen, Anke Samel-Pommerencke, David Fechtig, Ann E. Ehrenhofer-Murrayfor western blotting were α-H4 K16Ac (Millipore 05–1232), α-myc (Sigma M4439) and α-GAPDH (loading control, Abcam ab9385). The immunoblots were imaged on a Bio-Rad imaging system. Data accessibility ChIP-seq data was deposited

(13) Plasmodium infection prevents recurrence and metastasis of hepatocellular carcinoma possibly via inhibition of the epithelial‑mesenchymal transitionMolecular Medicine ReportsMarch 31, 2021Yun Liang et al.animal suffering. Antibodies. Western blotting was performed using the following primary antibodies: GAPDH (cat. no. ab9385; Abcam), E‑cadherin (cat. no. BS1098; Bioworld Technology, Inc.), Snail (cat. no. 3879S; Cell Signaling Technology

(14) Dominant-negative NFKBIA mutation promotes IL-1β production causing hepatic disease with severe immunodeficiencyThe Journal of clinical investigationNovember 2, 2020Tan EE, Hopkins RA, Lim CK, Jamuar SS, Ong C, Thoon KC, Koh MJ, Shin EM, Lian DW, Weerasooriya M, et al.as previously stated, followed by incubation with anti–α-tubulin (ab18251, Abcam) or anti–GAPDH-HRP (ab9385, Abcam) for 16 hours at 4°C. Detection of bands occurred as previously described. Immunofluorescence.

(15) A Composite Tissue Engineered Bone Material Consisting of Bone Mesenchymal Stem Cells, Bone Morphogenetic Protein 9 (BMP9) Gene Lentiviral Vector, and P3HB4HB Thermogel (BMSCs-LV-BMP9-P3HB4HB) Repairs Calvarial Skull Defects in Rats by Expression of Osteogenic FactorsMedical Science MonitorSeptember 7, 2020Cheng Zhou, Chuan Ye, Chen Zhao, Junyi Liao, Yuwan Li, Hong Chen, Wei Huang4°C, followed by incubation with horseradish peroxidase (HRP)-labeled rabbit antimouse IgG (Cat. No. ab9385) or HRP-labeled goat anti-rabbit IgG (Cat. No. ab6721) at 37°C for 2 h. All of the above primary and

(16) miR-143-3p impacts on pulmonary inflammatory factors and cell apoptosis in mice with mycoplasmal pneumonia by regulating TLR4/MyD88/NF-κB pathwayBioscience ReportsJuly 31, 2020Yongjun Wang, Huan Li, Yongsheng Shi, Shuying Wang, Yan Xu, Hanyi Li, Donghai LiuU.K.), Bax (1:1000, ab8805, Abcam, U.S.A.), Bcl-2 (1:1000, ab32124, Abcam, U.S.A.) and GAPDH (1:5000, ab9385, Abcam, U.S.A.). After the membrane was washed three times, it was added with horseradish peroxidase-labeled

(17) LncRNA TTN-AS1 Promotes Progression of Non-Small Cell Lung Cancer via Regulating miR-491-5p/ZNF503 AxisOncoTargets and TherapyJuly 1, 2020Guanbin Qi, Lei LiAbcam), E-cadherin (ab15148, Abcam), N-cadherin (ab18203, Abcam), Vimentin (ab137321, Abcam) and GAPDH (ab9385, Abcam). For cell migration assay, cells were placed in the upper chamber with serum-free medium. Besides,

(18) Monocytes enhance the inflammatory response to TLR2 stimulation in aortic valve interstitial cells through paracrine up-regulation of TLR2 levelInternational journal of biological sciencesJanuary 1, 2020Zhang P, The E, Nedumaran B, Ao L, Jarrett MJ, Xu D, Fullerton DA, Meng X.obtained from Novus Biologicals, LLC (Centennial, CO). Antibodies against β-actin (ab49900) and GAPDH (ab9385) were obtained from Abcam (Cambridge, MA). Antibody against vimentin (ab137321) was obtained from Abcam

(19) Glioma stem cells-derived exosomal miR-26a promotes angiogenesis of microvessel endothelial cells in gliomaJournal of Experimental & Clinical Cancer ResearchDecember 1, 2019Zhi-Fei Wang, Fan Liao, Hao Wu, Jin DaiInc.. Cambridge, MA, USA), p-Akt (1: 100, ab192623, Abcam Inc.. Cambridge, MA, USA), and GAPDH (1: 100, ab9385, Abcam Inc.. Cambridge, MA, USA) (internal reference) for 1 h at 37 °C. TBST washes (5 min × 3) were

(20) Low‑frequency ultrasound enhances vascular endothelial growth factor expression, thereby promoting the wound healing in diabetic ratsExperimental and Therapeutic MedicineSeptember 25, 2019Lang Chen, Qian Zheng, Xianzhuo Chen, Jun Wang, Lan Wang1:1,000), anti‑rabbit IL‑6 (Abcam; cat. no. ab208113; 1:500) and anti‑rabbit GAPDH (Abcam; cat. no. ab9385; 1:1,000). The immune complexes were then immunoblotted with horseradish peroxidase‑conjugated anti‑mouse

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8/23/2016Good Antibody, With Limited Use Rebecca WatesWestern blot assays to validate protein array data in ovarian cancer cell lines.

Anti-GAPDH antibody - Loading Control (HRP) from Abcam

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Anti-GAPDH antibody - Loading Control (HRP) from Abcam

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