Western blot analysis of extracts of HT-29 cells, using Chk1 antibody (TA374724) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

Immunofluorescence analysis of NIH/3T3 cells using Chk1 Rabbit pAb (TA374724) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using Chk1 Rabbit pAb (TA374724) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.