Alexa Fluor(R) 700 anti-mouse CD3 100215 from BioLegend

Product Specs
ItemAlexa Fluor(R) 700 anti-mouse CD3
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100215
NCBI Gene AliasesCtg-3, Ctg3, T3g
ReactivityMouse
Quantity25 µg
IsotypeIgG2b, Kappa
Clone17A2
HostRat
ApplicationsFlow Cytometry (FC)
Conjugate/TagAlexa Fluor® 700
Antibody TypePrimary
NCBI Full Gene NameCD3 antigen, gamma polypeptide
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenγδTCR-positive T-T hybridoma D1
ClonalityMonoclonal
TargetCD3

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Hematopoietic 12/15-lipoxygenase activity negatively contributes to fungal-associated allergic asthmaAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologyAugust 1, 2023Mgayya Makullah, Diandra A. Ellis, MaryJane Jones, Chad SteeleM5/114.15.2); CD4 T cells as CD45 + CD3+CD4 + TCRb+ using anti-CD45, anti-CD3 (BioLegend, Cat. No. 100216, clone 17A2), anti–CD4 (BioLegend, Cat. No. 100422, clone GK1.5), and anti–TCRb (BioLegend, Cat. No.

(2) Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during chronic infectionResearch squareJune 15, 2023Zheng W, Chang IC, Limberis J, Budzik J, Zha BS, Howard Z, Chen L, Ernst J.104522), CD44-PE/Cy7 (BioLegend, 103030), CD154-APC (BioLegend, 106510), CD3-Alexa Fluor 700 (BioLegend, 100216). Antibodies for intracellular markers were: CD107a (LAMP1)-Alexa Fluor 594 (BioLegend, 121622), ATP6V1B2-Alexa

(3) Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during chronic infectionbioRxiv : the preprint server for biologyMay 28, 2023Zheng W, Chang IC, Limberis J, Budzik JM, Zha BS, Howard Z, Chen L, Ernst JD.104522), CD44-PE/Cy7 (BioLegend, 103030), CD154-APC (BioLegend, 106510), CD3-Alexa Fluor 700 (BioLegend, 100216). Antibodies for intracellular markers were: CD107a (LAMP1)-Alexa Fluor 594 (BioLegend, 121622), ATP6V1B2-Alexa

(4) Designing epitope-focused vaccines via antigen reorientationbioRxivDecember 24, 2022Duo Xu, Chunfeng Li, Ashley Utz, Payton A.B. Weidenbacher, Shaogeng Tang, Mrinmoy Sanyal, Bali Pulendran, Peter S. Kim(Tonbo Biosciences), anti-mouse CD16/32 (clone 2.4G2, BD Biosciences #553142), CD3 (clone 17A2, BioLegend #100216), CD4 (clone GK1.5, BioLegend #100469), CXCR5 (clone L138D7, BioLegend #145529), PD1 (clone 29F.1A12,

(5) Extended SARS-CoV-2 RBD booster vaccination induces humoral and cellular immune tolerance in miceiScienceDecember 22, 2022Feng-Xia Gao, Rui-Xin Wu, Mei-Ying Shen, Jing-Jing Huang, Ting-Ting Li, Chao Hu, Fei-Yang Luo, Shu-Yi Song, Song Mu, Ya-Nan Hao, et al.anti-mouse/human CD45R/B220 Antibody,BioLegend,Cat#103229; AB_492875 Alexa Fluor 700 anti-mouse CD3,BioLegend,Cat#100216; AB_493697 PE anti-mouse CD8a,BioLegend,Cat#162303; AB_2894434 Brilliant Violet 605™ anti-mouse CD69,BioLegend,Cat#104529;AB_11203710 APC anti-mouse CD137

(6) The regional distribution of resident immune cells shapes distinct immunological environments along the murine epididymiseLifeDecember 14, 2022Pleuger C, Ai D, Hoppe ML, Winter LT, Bohnert D, Karl D, Guenther S, Epelman S, Kantores C, Fijak M, et al.AB_893344,FC (1:200) for infection study Antibody,AF700 anti-mouse CD3 (rat monoclonal),BioLegend,Cat.No.: 100216; RRID: AB_493697,FC (1:50) for infection study Antibody,Brilliant Violet 511 anti-mouse/ human CD45R/B220

(7) Docosahexaenoic acid supplementation represses the early immune response against murine cytomegalovirus but enhances NK cell effector functionBMC ImmunologyDecember 1, 2022Shuting Wu et al.CD62L,MEL-14,BioLegend,Cat#104438 Anti-mouse CD45,30-F11,BioLegend,Cat#103154 Anti-mouse CD3,17A2,BioLegend,Cat#100216 Cat#100320 Anti-mouse CD19,605,BioLegend,Cat#115520 Cat#115528 Anti-mouse GZMB,GB11,BioLegend,Cat#515406 Anti-mouse

(8) Inhibiting DNA methylation and RNA editing upregulates immunogenic RNA to transform the tumor microenvironment and prolong survival in ovarian cancerJ Immunother CancerNovember 7, 2022Stephanie Gomez, Olivia L Cox, Reddick R Walker, Uzma Rentia, Melissa Hadley, Elisa Arthofer, Noor Diab, Erin E Grundy, Tomas Kanholm, James I McDonald, et al.141708), CD11c-BV421 (BioLegend, 117343), CD40-PE/Cy7 (BioLegend, 124622), CD3-AlexaFluor 700 (BioLegend, 100216), B220-AlexaFluor 700 (BioLegend, 103232), NK1.1-AlexaFluor 700 (BioLegend, 108730). After staining,

(9) Autoimmune neuroinflammation triggers mitochondrial oxidation in oligodendrocytesGliaNovember 1, 2022Steudler J, Ecott T, Ivan DC, Bouillet E, Walthert S, Berve K, Dick TP, Engelhardt B, Locatelli G.the following antibodies diluted in FACS buffer: CD3 (AF700 anti‐mouse, clone 17A2, Biolegend, Cat. 100216), CD11b (PercP anti‐mouse/human, clone M1/70, Biolegend, Cat. 101230), CD45 (PE/Cy7 anti‐mouse, clone

(10) Amphiphile-CpG vaccination induces potent lymph node activation and COVID-19 immunity in mice and non-human primatesNPJ vaccinesOctober 28, 2022Seenappa LM, Jakubowski A, Steinbuck MP, Palmer E, Haqq CM, Carter C, Fontenot J, Villinger F, McNeil LK, DeMuth PC.1:125), CD11c (BV421, clone: N418, BioLegend cat# 117330, 1:40), CD3 (AF700, clone: 17A2, BioLegend cat# 100216, 1:100), CD19 (AF700, clone: 6D5, BioLegend cat# 115528, 1:100), Ly6C (BV650, clone: HK1.4, BioLegend

(11) Mucosal chemokine adjuvant enhances synDNA vaccine-mediated responses to SARS-CoV-2 and provides heterologous protection in vivoCell Reports MedicineJuly 19, 2022Ebony N. Gary et al.AB_2563056 anti-mouse CD4,Biolegend,Catalog number: 100447 RRID: AB_2564586 anti-mouse CD3e,Biolegend,Catalog number: 100216 RRID: AB_493697 anti-mouse CD107a,Biolegend,Catalog number: 121606 RRID: AB_572007 anti-mouse IFNγ,Biolegend,Catalog

(12) Tfh Exosomes Derived from Allergic Rhinitis Promote DC Maturation Through miR-142-5p/CDK5/STAT3 PathwayJournal of Inflammation ResearchMay 31, 2022Zhen-Xiao Teng, Xuan-Chen Zhou, Run-Tong Xu, Fang-Yuan Zhu, Xin Bing, Na Guo, Lei Shi, Wen-Wen Qi, Cheng-Cheng Liu, Ming Xiaexosomes of Tfhs promote DC maturation. Materials and Methods Reagents AF700 Rat Anti-Mouse CD3 (Cat# 100215), FITC Rat Anti-Mouse CD44 (Cat# 103005), BV605 Rat Anti-Mouse CD8 (Cat#100743) and 7AAD (Cat#420403)

(13) Elimination of receptor binding by influenza hemagglutinin improves vaccine-induced immunityNPJ vaccinesApril 11, 2022Hendin HE, Lavoie PO, Gravett JM, Pillet S, Saxena P, Landry N, D'Aoust MA, Ward BJ.containing the following anti-mouse antibodies: anti-CD3 Alexa Fluor 700 (1 µl/sample; Biolegend Cat. No. 100216), anti-CD4 V500 (1 µl/sample; BD Cat. No. 560782), anti-CD19 PE-CF594 (1 µl/sample; BD Cat. No. 562291),

(14) Enhanced T-Cell Priming and Improved Anti-Tumor Immunity through Lymphatic Delivery of Checkpoint Blockade ImmunotherapyCancers (Basel)April 4, 2022Carolina Mantilla-Rojas, Fred C. Velasquez, Janelle E. Morton, Leticia C. Clemente, Edwin R. Parra, Carlos Torres-Cabala, Eva M. Sevick-Muraca(Biolegend, San Diego, CA, USA, Cat: 103126); CD3, Alexa Fluor 700 anti-mouse CD3 (Biolegend, Cat: 100216); CD4, BV650 anti-mouse CD4 (Biolegend, Cat: 100469); CD8a, Alexa Fluor 488 anti-mouse CD8a (Biolegend,

(15) PU.1 Expression Defines Distinct Functional Activities in the Phenotypic HSC Compartment of a Murine Inflammatory Stress ModelCellsFebruary 15, 2022Chavez JS, Rabe JL, Hernandez G, Mills TS, Niño KE, Davizon-Castillo P, Pietras EM.CD8-PE (Biolegend, 100708), Mac-1 PE/Cy7, IgM APC (eBioscience, 17-1590-82), CD3 AF700 (Biolegend, 100216), CD19 APC/Cy7 (Biolegend, 115530), Gr1 PB (Biolegend, 108430), CD4 BV510 (Biolegend, 100449), Ly6C

(16) Identification of Sorafenib as a Treatment for Type 1 DiabetesFrontiers in ImmunologyFebruary 15, 2022Qin Zeng, Jianfeng Song, Dandan Wang, Xiaoxiao Sun, Yalun Xiao, Haowei Zhang, Yang Xiao, Zhiguang Zhou, Tuo Dengused: CD45 (Biolegend, Cat#103108, 103132 and BD, Cat#562420), CD3e (Biolegend, Cat#100330, 100306, 100216), CD4 (Biolegend, Cat#100406, 100408, 100414, 980804 and eBioscience, Cat#11-0049-41), CD8a (Biolegend,

(17) Elevated granulocyte-colony stimulating factor and hematopoietic stem cell mobilization in Niemann-Pick type C1 diseaseJournal of lipid researchFebruary 1, 2022Groenen AG, La Rose AM, Li M, Bazioti V, Svendsen AF, Kloosterhuis NJ, Ausema A, Pranger A, Heiner-Fokkema MR, Niezen-Koning KE, et al.

(18) Intranasal Immunization With a c-di-GMP-Adjuvanted Acellular Pertussis Vaccine Provides Superior Immunity Against Bordetella pertussis in a Mouse ModelFrontiers in immunologyJanuary 1, 2022Jiang W, Wang X, Su Y, Cai L, Li J, Liang J, Gu Q, Sun M, Shi L.cells, lung cells were stained for cell surface markers with anti-CD3 (Biolegend, clone: 17A2, Cat# 100216), anti-CD4 (Biolegend, clone: GK1.5, Cat# 100406), anti-CD8 (Biolegend, clone: 53-6.7, Cat# 100752),

(19) A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challengeCell reportsDecember 14, 2021Jiang W, Shi L, Cai L, Wang X, Li J, Li H, Liang J, Gu Q, Ji G, Li J, et al.AB_2734767 MHC Class II (I-A/I-E)-PE,Invitrogen,Cat# 12-5321-82; AB_465928 CD3-AF700,Biolegend,Cat# 100216; RRID: AB_493697 CD4-FITC,Biolegend,Cat# 100406; RRID: AB_312691 CD8-BV510,Biolegend,Cat# 100752;

(20) Pulmonary immune cell trafficking promotes host defense against alcohol-associated Klebsiella pneumoniaCommunications BiologyDecember 1, 2021Derrick R. Samuelson, Min Gu, Judd E. Shellito, Patricia E. Molina, Christopher M. Taylor, Meng Luo, David A. Welshantibodies specific for murine CD45 (0.25 μg per test, Cat #103104, BioLegend), CD3e (2.5 μg per test, Cat #100216, BioLegend), CD4 (1 μg per test, Cat #564667, BD), CD8a (1 μg per test, Cat #100748, BioLegend), GR1

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(1)

3/29/2019Reliable CD3 Antibody for Mouse Rebecca GuyerThis is a reliable antibody to use for CD3+ staining in mouse cells.
(1)

12/20/2018Alexa Fluor 700 Anti-Mouse CD3 Antibody Anup KumarI am studying molecular anomalies in signaling pathways leading to cancers and the development of anti-cancer immune therapies. This antibody is great for detecting CD3 on mouse immune cells.
(1)

6/18/2018Anti-Mouse CD3 Matthew RatsepI've used this antibody in a number of flow cytometry assays in mouse samples. I've always produced good results with this antbody. The only issue I have with it is when doing fix-perm assays this ... Read Review
(1)

4/6/2016CD3 Receptor Antibody Laura BaucherMouse peripheral lymph node cells were used to stain for CD3 receptor in combination with other cell surface markers. Since CD3 was to be checked on a specific class of cells with stronger color, AF-... Read Review
(1)

9/30/2015AF700 Anti Mouse CD3 in Flow Cytometry for Mouse Tissue Yanxia CaoThe aim of this experiment was to detect cytokines in mouse T cells. This antibody is good to distinguish the T cell population.

Alexa Fluor(R) 700 anti-mouse CD3 from BioLegend

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Alexa Fluor(R) 700 anti-mouse CD3 from BioLegend

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