Alexa Fluor(R) 488 anti-mouse I-A/I-E 107615 from BioLegend

Product Specs
ItemAlexa Fluor(R) 488 anti-mouse I-A/I-E
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number107615
NCBI Gene AliasesH-2Eb, Rmcs1, Ia2, I-Abeta, IAb, Eb, H-2Ab, Abeta, H2Eb, Ia-4, H2-Ab, Ia4, Ia-2
Conjugate/TagAlexa Fluor® 488
Quantity25 µg
ApplicationsFlow Cytometry (FC), 3D Immunohistochemistry (IHC)
HostRat
IsotypeIgG2b, Kappa
ReactivityMouse
CloneM5/114.15.2
Antibody TypePrimary
NCBI Full Gene Namehistocompatibility 2, class II antigen A, beta 1, histocompatibility 2, class II antigen E beta
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenActivated C57BL/6 mouse spleen cells
ClonalityMonoclonal
TargetI-A/I-E

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BioLegend

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Website: biolegend.com

(1) Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networksImmunityAugust 8, 2023Ugur M, Labios RJ, Fenton C, Knöpper K, Jobin K, Imdahl F, Golda G, Hoh K, Grafen A, Kaisho T, et al.50-0443-82; RRID: AB_10597449 Rat anti-MHC-II (Alexa Fluor 488-conjugated),"M5/114.15.2, BioLegend",Cat# 107616; RRID: AB_493523 Rat anti-MHC-II (Alexa Fluor 700-conjugated),"M5/114.15.2, BioLegend",Cat# 107622;

(2) Protection against tuberculosis achieved by dissolving microneedle patches loaded with live Mycobacterium paragordonae in a BCG prime-boost strategyFrontiers in ImmunologyJune 16, 2023Mi-Hyun Lee, Hyejun Seo, Moon-Su Lee, Byoung Jun Kim, Hye Lin Kim, Du Hyung Lee, Jaehun Oh, Ju Yeop Shin, Ju Young Jin, Do Hyeon Jeong, et al.fixed with 4% PFA for 20 min at room temperature and stained with Alexa Fluor® 488 MHCII (Biolegend, #107615), langerin Alexa Fluor® 594 (Santa Cruz, #SC-271272 AF594) or Alexa Fluor® 488 Ki-67 (Biolegend, #151204)

(3) Characterization of immune cell populations in syngeneic murine tumor modelsCancer MedicineMay 1, 2023Sofie Snipstad, Frida Bremnes, Mats Dehli Haugum, Einar SulheimCl:A3‐1)",Macrophages,1:25 B,"Anti‐CD206 (biolegend, 141707; Clone C068C2)",M2‐like macrophages,1:60 B,"Anti‐MHCII (BioLegend, 107616; Clone M5/114.15.2)",MHCII high macrophages,1:250 B,"Anti‐CD19 (BioLegend, 115519; Clone 6D5)",B cells,1:122 Antibodies

(4) Osteopontin Promotes Macrophage M1 Polarization by Activation of the JAK1/STAT1/HMGB1 Signaling Pathway in Nonalcoholic Fatty Liver DiseaseJournal of Clinical and Translational HepatologyApril 28, 2023Zhihao Xu, Feiyang Xi, Xinxin Deng, Yuqi Ni, Changqin Pu, Dan Wang, Weiming Lou, Xufang Zeng, Ning Su, Chen Chen, et al.Proteintech (Rosemont, IL, USA). Rabbit PE-conjugated anti-mouse CD86 (Cat#: 159202) and MHCII (Cat#: 107615) antibodies were purchased from Biolegend (San Diego, CA, USA). IFNγ (Cat#: 315-05) was purchased from

(5) Systemic vaccination induces CD8+ T cells and remodels the tumor microenvironmentCellNovember 1, 2022Faezzah Baharom, Ramiro A. Ramirez-Valdez, Ahad Khalilnezhad, Shabnam Khalilnezhad, Marlon Dillon, Dalton Hermans, Sloane Fussell, Kennedy K.S. Tobin, Charles-Antoine Dutertre, Geoffrey M. Lynn, et al.(PE-Cy5); clone BM8,eBioscience,Cat# 15-4801-82 Anti-mouse IA/IE (AF488); clone M5/114.15.2,Biolegend,Cat# 107616 Anti-mouse LY6A/E (PE-CF594); clone D7,BD Biosciences,Cat# 562730 Anti-mouse Ly6C (APC-eF780); clone

(6) An improved organotypic cell culture system to study tissue-resident macrophages ex vivoCell Rep MethodsAugust 22, 2022Aktories P, Petry P, Glatz P, Andrieux G, Oschwald A, Botterer H, Gorka O, Erny D, Boerries M, Henneke P, et al.155505;RRID: AB_2750234 "Alexa Fluor 488 anti-mouse I-A/I-E Antibody, Clone M5/114.15.2",BioLegend,Cat# 107616; RRID: AB_493523 "Alexa Fluor 700 anti-mouse I-A/I-E Antibody, Clone M5/114.15.2",Thermo Fisher,Cat

(7) DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potentialDevelopmental cellAugust 22, 2022Frederico B, Martins I, Chapela D, Gasparrini F, Chakravarty P, Ackels T, Piot C, Almeida B, Carvalho J, Ciccarelli A, et al.(M5/114.15.2),BioLegend,Cat# 107618; RRID: AB_493525 AF488 anti-mouse I-A/I-E (M5/114.15.2),BioLegend,"Cat# 107616, RRID:AB_493523" eF450 anti-mouse I-A/I-E (M5/114.15.2),Thermo Fisher Scientific,"Cat# 48-5321-82,

(8) Proinflammatory signaling in islet β cells propagates invasion of pathogenic immune cells in autoimmune diabetesCell reportsJune 28, 2022Piñeros AR, Kulkarni A, Gao H, Orr KS, Glenn L, Huang F, Liu Y, Gannon M, Syed F, Wu W, et al.RRID:AB_10736472 anti-TNF-α Antibody,Biolegend,Cat# 506314: RRID:AB_493330 anti-MHC-II,Biolegend,Cat# 107616: RRID:AB_493523 anti-CD80,Biolegend,Cat# 104718: RRID:AB_492824 anti-CD86,Biolegend,Cat# 105008: RRID:AB_313151 anti-CD4-FITC,Biolegend,Cat#

(9) The transcription factor HIF-1α mediates plasticity of NKp46+ innate lymphoid cells in the gutThe Journal of experimental medicineFebruary 7, 2022Krzywinska E, Sobecki M, Nagarajan S, Zacharjasz J, Tambuwala MM, Pelletier A, Cummins E, Gotthardt D, Fandrey J, Kerdiles YM, et al.117306), anti-CD8 (53-6.7; 100730), anti-CD19 (6D5; 115530; 152404), anti-MHCII (M5/114.15.2; 48-5321; 107616), anti-Ly6G (1A8; 746448; 127618), anti-Ly6C (HK1.4; 128035), anti-CD45 (30-F11; 564225; 103128), anti-CD4

(10) An immunologically active, adipose-derived extracellular matrix biomaterial for soft tissue reconstruction: concept to clinical trialNPJ Regenerative medicineJanuary 14, 2022Anderson AE, Wu I, Parrillo AJ, Wolf MT, Maestas DR Jr, Graham I, Tam AJ, Payne RM, Aston J, Cooney CM, et al.BioLegend), F4/80 PE-Cy7 (1:250, Cat. No. 123113, BioLegend), MHCII I-A/I-E Alexa Fluor 488 (1:200, Cat. No. 107615, BioLegend), Siglec-F PE-594 (1:200, Cat. No. 562757, BD Biosciences), CD206 PE (1:250, Cat. No. 141705,

(11) The allergy mediator histamine confers resistance to immunotherapy in cancer patients via activation of the macrophage histamine receptor H1Cancer cellJanuary 10, 2022Li H, Xiao Y, Li Q, Yao J, Yuan X, Zhang Y, Yin X, Saito Y, Fan H, Li P, et al.CD206 PE,BioLegend,Cat# 141706; RRID: AB_10895754 Anti-mouse I-A/I-E Alexa Fluor® 488,BioLegend,Cat# 107616; RRID: AB_493523 Anti-mouse VISTA PE/Cyanine7,BioLegend,Cat# 143714; RRID: AB_2632662 Anti-mouse CD366

(12) Intercellular cGAMP transmission induces innate immune activation and tissue inflammation in Trex1 deficiencyiScienceAugust 20, 2021Jütte BB, Krollmann C, Cieslak K, Koerber RM, Boor P, Graef CM, Bartok E, Wagner M, Carell T, Landsberg J, et al.

(13) Skin-resident immune cells actively coordinate their distribution with epidermal cells during homeostasisNature cell biologyMay 1, 2021Park S, Matte-Martone C, Gonzalez DG, Lathrop EA, May DP, Pineda CM, Moore JL, Boucher JD, Marsh E, Schmitter-Sánchez A, et al.118101), purified rat anti-mouse MHC class II or MHC class II Alexa 488 (BioLegend; 107602, 1:100; 107616, 1:100), purified rat anti-mouse CD45 (Biolegend; 103102, 1:100), purified mouse anti-mouse/human CD207

(14) Nonpeptidergic neurons suppress mast cells via glutamate to maintain skin homeostasisCellApril 15, 2021Zhang S, Edwards TN, Chaudhri VK, Wu J, Cohen JA, Hirai T, Rittenhouse N, Schmitz EG, Zhou PY, McNeil BD, et al.

(15) In vitro and ex vivo evaluation of tumor-derived exosome-induced dendritic cell dysfunction in mouseSTAR protocolsMarch 19, 2021Zeng W, Yin X, Jiang Y, Jin L, Liang W.PE/Cy7,BioLegend,Cat # 107630; RRID: AB_2069376,Cat # 107630; RRID: AB_2069376 Anti-mouse MHC II FITC,BioLegend,Cat # 107615; RRID: AB_493524,Cat # 107615; RRID: AB_493524 Anti-mouse CD45 BV605,BioLegend,Cat # 103139; RRID:

(16) GSK2593074A blocks progression of existing abdominal aortic dilationJVS-Vascular ScienceFebruary 11, 2021Mitri K. Khoury, Ting Zhou, Huan Yang, Samantha R. Prince, Kartik Gupta, Amelia R. Stranz, Qiwei Wang, Bo Liu(AF2535, R&D Systems, Minneapolis, Minn), anti-F4/80 (123120, Biolegend, San Diego, Calif), anti-MHCII (107616, Biolegend), anti-MYH11 (ab53219, Abcam, Cambridge, UK), and anti-IL-1β (AF-401, R&D Systems). A total

(17) Competition for Active TGFβ Cytokine Allows for Selective Retention of Antigen-Specific Tissue- Resident Memory T Cells in the Epidermal NicheImmunityJanuary 12, 2021Hirai T, Yang Y, Zenke Y, Li H, Chaudhri VK, De La Cruz Diaz JS, Zhou PY, Nguyen BA, Bartholin L, Workman CJ, et al.(MP6-XT22),BioLegend,"Cat# 506328, RRID:AB_2562902" Alexa Fluor 488 anti-mouse I-A/I-E (M5/114.15.2),BioLegend,"Cat# 107616, RRID:AB_493523" Alexa Fluor 647 anti-human CD271 (NGFR) (ME20.4),BioLegend,"Cat# 345114, RRID:AB_2572059" PE anti-human CD271 (NGFR

(18) Local Targeting of Lung-Tumor-Associated Macrophages with Pulmonary Delivery of a CSF-1R Inhibitor for the Treatment of Breast Cancer Lung MetastasesMolecular pharmaceuticsDecember 7, 2020Alhudaithi SS, Almuqbil RM, Zhang H, Bielski ER, Du W, Sunbul FS, Bos PD, da Rocha SRP.PerCP-Cy5.5 anti-mouse I-A/I-E (catalog# 107625), Alexa Fluor® 488 anti-mouse I-A/I-E (kind gift, catalog# 107615), FITC anti-mouse CD80 (catalog# 104705), and Alexa Fluor® 488 anti-mouse CD206 (catalog# 141709) were

(19) Myeloid-Derived TSP1 (Thrombospondin-1) Contributes to Abdominal Aortic Aneurysm Through Suppressing Tissue Inhibitor of Metalloproteinases-1Arteriosclerosis, thrombosis, and vascular biologyDecember 1, 2020Yang H, Zhou T, Sorenson CM, Sheibani N, Liu B.sections were blocked with 10% normal donkey serum. Alexa Fluor® 488 anti-mouse MHC II antibody (1:100, 107616, clone M5/114.15.2, BioLegend) were applied onto tissue sections, and incubated 2 hours at room temperature.

(20) Maternal ω3 docosapentaenoic acid inhibits infant allergic dermatitis through TRAIL-expressing plasmacytoid dendritic cells in miceAllergyAugust 1, 2020Hirata SI, Nagatake T, Sawane K, Hosomi K, Honda T, Ono S, Shibuya N, Saito E, Adachi J, Abe Y, et al.Alexa Fluor 488–conjugated anti‐mouse B220 (BD Biosciences, 557669) and MHC class II mAb (BioLegend, 107616) and anti‐human CD8α mAb (BioLegend, 300916); Alexa Fluor 647–conjugated anti‐mouse plasmacytoid dendritic cell

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5/6/2020Super Easy I-A/I-E Antibody for IHC Scott ScarneoThis is easy and very selective for macrophages.

Alexa Fluor(R) 488 anti-mouse I-A/I-E from BioLegend

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Alexa Fluor(R) 488 anti-mouse I-A/I-E from BioLegend

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