Fig 1: The shape of the nasal OC is compromised in Foxg1-/- mutants. Double immunofluorescence for Coup-TFI and Mitf in wild-type (Foxg1+/+) ventral (A–C) and dorsal (D–F) and Foxg1-/- ventral (A'–C') and dorsal (D'–F') E10.5 horizontal sections. Coup-TFI is found in the ventral (A, A') and dorsal (D, D') retina (r) and in the OS in both wild-types (A, D) and Foxg1-/- mutants (A', D'). Mitf is found in the RPE in ventral and dorsal sections in both wild-types (B, E) and Foxg1-/- mutants (B', E'). B, B', Brackets indicate the presumptive RPE (pRPE) in wild-types and Foxg1-/- mutants, respectively. C, *OF in wild-types, which is not clearly visible in Foxg1-/- mutants (C', ?). C', Double arrow indicates the abnormally enlarged distance between nasal and temporal OS in the Foxg1-/- mutant. Arrowhead indicates the lack of invagination of the nasal OC. F, F', Dashed lines indicate the nasotemporal axis. pMLC2 expression in wild-types (G) and Foxg1-/- mutants (G') is detected along the RPE (G, G', small arrows) and in the hinge region (G, G', vertical arrow). Scale bars: A–F, A'–F', 100 µm; G, G', 50 µm.