Anti-LAMP2 antibody [H4B4] - Lysosome Marker ab25631 from Abcam

Product Specs
ItemAnti-LAMP2 antibody [H4B4] - Lysosome Marker
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab25631
Antibody TypePrimary
HostMouse, Nude mouse
CloneH4B4
Quantity100 UG
ClonalityMonoclonal
IsotypeIgG1
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
Concentration0.1 mg/ml
Gene NameLAMP2
ReactivityAfrican green monkey, Human
TargetLAMP2

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) The cell type dependent sorting of CD9- and CD81 to extracellular vesicles can be exploited to convey tumor sensitive cargo to target cellsDrug deliveryDecember 1, 2023Zuppone S, Zarovni N, Vago R.following antibodies: cathepsin B (1:800, 12216-1-AP, Proteintech), Lamp2b (1:1000, H4B4 clone, Abcam, ab25631), CD63 (1:1000, BD Pharmingen, #556019), CD9 (1:1000, BD Pharmingen, #555370), CD81 (1:1000, BD Pharmingen,

(2) L-Type Amino Acid Transporter 1 (LAT1) Promotes PMA-Induced Cell Migration through mTORC2 Activation at the LysosomeCellsOctober 23, 2023Kun Tae, Sun-Jick Kim, Sang-Woo Cho, Hoyeon Lee, Hyo-Sun Cha, Cheol-Yong Choiwas purchased from Roche. Anti-HA (sc-7392) was purchased from Santa Cruz Biotechnology. Anti-LAMP2 (ab25631) and anti-sodium potassium ATPase (ab76020) were purchased from Abcam. 2.4. Immunoblotting Cells were

(3) Enhanced microglial dynamics and paucity of tau seeding in the amyloid plaque microenvironment contributes to cognitive resilience in Alzheimer’s diseasebioRxivAugust 1, 2023Nur Jury-Garfe, Yanwen You, Pablo Martínez, Javier Redding-Ochoa, Hande Karahan, Travis S. Johnson, Jie Zhang, Jungsu Kim, Juan C. Troncoso, Cristian A. Lasagna-Reeves(1:100, MN1020, Invitrogen), anti-ARP2 (E12) (1:100, sc-166103, Santa Cruz) and anti-LAMP2 (H4B4) (1:300, ab25631, Abcam). The next day, sections were quickly washed three times in TBS and incubated for 2 h with a

(4) Clinical-grade human umbilical cord-derived mesenchymal stem cells improved skeletal muscle dysfunction in age-associated sarcopenia miceCell Death & DiseaseMay 12, 2023Chao Wang, Bichun Zhao, Jinglei Zhai, Ailin Wang, Ning Cao, Tuling Liao, Ruyu Su, Lijuan He, Yanhua Li, Xuetao Pei, et al.ab199010), Anti-alpha smooth muscle Actin (Abcam, USA, ab5694), Anti-LAMP2-Lysosome Marker (Abcam, USA, ab25631), Anti-Beta actin (Abcam, USA, ab8226), Anti-Cleaved LC3B (Sigma, Germany, L7S43), Anti-ARPC5/p16 ARC

(5) CircLASP1 silence strengthens the therapeutic effects of MK‐2206 on nasopharyngeal cancer through upregulating miR‐625Cancer ScienceMay 1, 2023Li‐Na Xu, Si‐Le Liu, Yang Yang, Lu Shu, Yi SunTriton X‐100. After being blocked with 5% BSA in PBS, cells were incubated with anti‐LAMP2 (1:1000, ab25631; Abcam) and anti‐LC3 (1:2000, #12741; Cell Signaling Technology) antibodies at 4°C overnight. Cells

(6) A druggable copper-signalling pathway that drives inflammationNatureMay 1, 2023Solier S, Müller S, Cañeque T, Versini A, Mansart A, Sindikubwabo F, Baron L, Emam L, Gestraud P, Pantoș GD, et al.127610, clone 1A8, lot B2559839, FCy 1:200, Ms), lysosome-associated membrane protein 2 (LAMP2) (Abcam, ab25631, clone H4B4, FM 1:400, Hu), NOS2–APC (eBioscience, 17-5920-82, clone CXNFT, lot 2154045, FCy 1:100,

(7) Common and Rare Variants in TMEM175 Gene Concur to the Pathogenesis of Parkinson's Disease in Italian PatientsMolecular neurobiologyApril 1, 2023Palomba NP, Fortunato G, Pepe G, Modugno N, Pietracupa S, Damiano I, Mascio G, Carrillo F, Di Giovannantonio LG, Ianiro L, et al.overnight and at RT for 1 h, respectively. Anti-LAMP1 (ab25630, Abcam, Cambridge, UK, 1:20), anti-LAMP2 (ab25631, Abcam, Cambridge, UK, 1:100), anti-LC3B (2775, Cell Signaling, Danvers, MA, USA, 1:200), anti-TMEM175

(8) MiR-21-5p regulates the dynamic of mitochondria network and rejuvenates the senile phenotype of bone marrow stromal cells (BMSCs) isolated from osteoporotic SAM/P6 miceStem cell research & therapyMarch 29, 2023Sikora M, Śmieszek A, Pielok A, Marycz K.processes. Additionally, the specimens were stained with anti-LAMP2 antibody (H4B4) produced in mouse (ab25631, Abcam, Cambridge, UK) at the concentration 1:100 that serves as a marker of selective autophagy; anti-Ki67

(9) RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradationCommunications biologyFebruary 23, 2023Zhang L, Yu J, Zheng M, Zhen H, Xie Q, Zhang C, Zhou Z, Jin G.1:3000), anti-N-Cadherin rabbit mAb (CST, 13116; dilution 1:1000), anti-LAMP2[H4B4] mouse mAb (Abcam, ab25631; dilution 1:1000), anti-LAMP2A rabbit pAb (Abcam, ab18528; dilution 1:1000), anti-αTubulin mouse mAb

(10) Spatial proteomics reveals secretory pathway disturbances caused by neuropathy-associated TECPR2Nature communicationsFebruary 16, 2023Nalbach K, Schifferer M, Bhattacharya D, Ho-Xuan H, Tseng W, Williams LA, Stolz A, Lichtenthaler SF, Elazar Z, Behrends C.#MMS-101P/#901501), HS6ST2 (Abcam, # ab122220), LAMP1 (IF 1:100, Abcam/DSHB, #ab24170/H4A3), LAMP2 (IF 1:100, Abcam, #ab25631), LAMTOR1 (Cell Signaling, #8975S), LAMTOR2 (Cell Signaling, #8145); LAMTOR3 (Cell Signaling, #8168),

(11) Lipid and protein content profiling of isolated native autophagic vesiclesEMBO reportsDecember 6, 2022Schmitt D, Bozkurt S, Henning-Domres P, Huesmann H, Eimer S, Bindila L, Behrends C, Boyle E, Wilfling F, Tascher G, et al.(Nanotools, 0260‐100/LC3‐2G6); MAP1LC3B (Novus, NB100‐2220); SQSTM1 (Progen, GP62‐C); LAMP2 (Abcam, ab25631); TGN46 (BioRad, AHP500); HA (Sigma, H6908). Secondary antibody: Cy3 anti‐mouse (ImmunoResearch, 715‐165‐151),

(12) The ubiquitin-specific protease 8 antagonizes melatonin-induced endocytic degradation of MT(1) receptor to promote lung adenocarcinoma growthJournal of advanced researchNovember 1, 2022Sun Q, Zhang J, Li X, Yang G, Cheng S, Guo D, Zhang Q, Sun F, Zhao F, Yang D, et al.antibody (T5168, 1:100,000) was purchased from Sigma (Darmstadt, Germany). Mouse anti-LAMP2 antibody (ab25631, 1:50) was obtained from Abcam (Cambridge, UK). Mouse anti-Ki-67 (610969, 1:400) and anti-EEA1 (610456,

(13) Impairment of APPL1/Myoferlin facilitates adipogenic differentiation of mesenchymal stem cells by blocking autophagy flux in osteoporosisCellular and Molecular Life SciencesSeptember 1, 2022Yunhui Zhang et al.Santa Cruz Biotechnology Cat# sc-271901), anti-MYOF (1:100, Abcam Cat# ab178386), anti-LAMP2 (1:100, Abcam Cat# ab25631) or anti-Galectin 3 (1:100, Abcam Cat# ab209344) primary antibodies overnight at 4 °C. Next, anti-rabbit

(14) Thiosemicarbazones Can Act Synergistically with Anthracyclines to Downregulate CHEK1 Expression and Induce DNA Damage in Cell Lines Derived from Pediatric Solid TumorsInternational Journal of Molecular SciencesAugust 1, 2022Silvia Paukovcekova, Maria Krchniakova, Petr Chlapek, Jakub Neradil, Jan Skoda, Renata Veselskaantibodies Antigen,Type/Host,Clone,Catalog No.,Manufacturer,Dilution Pgp,Mono/Mo,F4,P7965,Sigma-Aldrich,1:100 LAMP-2,Mono/Mo,H4B4,ab25631,Abcam,1:100 Phospho-γH2A.X,Mono/Mo,3F2,MA1-2022,Invitrogen,1:100 Secondary antibodies,Secondary antibodies,Secondary

(15) Hydroxynonenal causes hepatocyte death by disrupting lysosomal integrity in non-alcoholic steatohepatitisCellular and Molecular Gastroenterology and HepatologyJuly 1, 2022Takuya Seike, Piyakarn Boontem, Masahiro Yanagi, Shihui Li, Hidenori Kido, Daisuke Yamamiya, Hidetoshi Nakagawa, Hikari Okada, Tatsuya Yamashita, Kenichi Harada, et al.following primary antibodies were used: a mouse monoclonal antibody against African green monkey LAMP2 (ab25631, Abcam, Cambridge, UK) at a dilution of 250, a rabbit monoclonal antibody against human cathepsin B

(16) Hypoxia-Reoxygenation Impairs Autophagy-Lysosomal Machinery in Primary Human Trophoblasts Mimicking Placental Pathology of Early-Onset PreeclampsiaInternational Journal of Molecular SciencesMay 18, 2022Shibin Cheng, Zheping Huang, Sukanta Jash, Kathleen Wu, Shigeru Saito, Akitoshi Nakashima, Surendra SharmaWaltham, MA, USA), anti-LAMP1 (1:1000; Abcam, ab25630, Waltham, MA, USA), anti-LAMP2 (1:1000; Abcam, ab25631, Waltham, MA, USA), Atg12 (D88H11) Rabbit mAb (1:1000; Cell Signaling Technology, #4180, Danvers, MA,

(17) Exclusion of NUMB Exon12 Controls Cancer Cell Migration through Regulation of Notch1-SMAD3 CrosstalkInternational Journal of Molecular SciencesApril 14, 2022Zheng Zhan, Ningyang Yuan, Xue You, Kai Meng, Rula Sha, Zhenzhen Wang, Qian Peng, Zhiqin Xie, Ruijiao Chen, Ying Fengwere purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies EEA1 (ab70521) and LAMP1 (ab25631) were from Abcam, and Notch1 (AF1546), Ubiquitin (AF1705) and Histone3 (AF0009) from Byotime (Nantong,

(18) Torin 1 alleviates impairment of TFEB-mediated lysosomal biogenesis and autophagy in TGFBI (p.G623_H626del)-linked Thiel-Behnke corneal dystrophyAutophagyApril 1, 2022Wang L, Zhao C, Zheng T, Zhang Y, Liu H, Wang X, Tang X, Zhao B, Liu P.and Company, 610,833); CTSD (Calbiochem, IM16); LC3 (Cell Signaling Technology, 3868); LAMP2 (Abcam, ab25631); TFEB (Cell Signaling Technology, 4240); TFEB (Abcam, ab270604); GAPDH (Protein Tech, 10,494-1-AP);

(19) Interindividual variability in transgene mRNA and protein production following adeno-associated virus gene therapy for hemophilia ANature medicineApril 1, 2022Fong S, Yates B, Sihn CR, Mattis AN, Mitchell N, Liu S, Russell CB, Kim B, Lawal A, Rangarajan S, et al.antibody (1:1,000 dilution; Cell Signaling Technology, C50B12) and anti-LAMP2 (1:100 dilution, Abcam, ab25631) diluted in Ventana Reaction Buffer (Ventana Medical Systems, 950-300). Slides were incubated overnight

(20) Active RB causes visible changes in nuclear organizationThe Journal of cell biologyMarch 7, 2022Krishnan B, Yasuhara T, Rumde P, Stanzione M, Lu C, Lee H, Lawrence MS, Zou L, Nieman LT, Sanidas I, et al.polyclonal antibody; 1:200; NB600-1384; Novus Biologicals; LAMP2 mouse monoclonal antibody; 1:100; ab25631; Abcam) were diluted in blocking buffer,added on coverslips, and incubated overnight. Coverslips were

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12/9/2019Good Antibody for Lysosome Staining in Human CellsDr. Min Hee OhGood and reliable antibody to look at lysosomes in human cells.

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Anti-LAMP2 antibody [H4B4] - Lysosome Marker from Abcam

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Anti-LAMP2 antibody [H4B4] - Lysosome Marker from Abcam

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