Fig 1: Serum NLRC5 concentrations negatively correlated with the proteinuria of IgAN patients. a Serum NLRC5 concentration in IgAN patients with different concentration of proteinuria (more than 1 g/day and less than 1 g/day); b Correlation analysis between serum NLRC5 concentration and proteinuria concentration. a by Ordinary one-way ANOVA; b by Pearson test. *P < 0.05, ****P < 0.0001
Fig 2: Serum NLRC5 concentrations were reduced in IgAN patients, had a negatively correlation with Lee’s grade. a The Serum NLRC5 concentration in IgAN patients and control subjects (tested); b The concentration of serum NLRC5 in Lee’s grade I-IV IgAN patients; c The correlation analysis between serum NLRC5 concentration and Lee’s grade; d Serum NLRC5 concentrations in controls, S1 and S0 of the Oxford classification. a by Unpaired t test; b and d by Ordinary one-way ANOVA test; c by Pearson test.*P < 0.05, ****P < 0.0001
Fig 3: The cut-off value of serum NLRC5 concentration was 1415 pg/ml. The cut-off value of serum NLRC5 concentration was 1415 pg/ml to distinguish IgAN patients from the area under the curve was 0.8445 (95% CI 0.725–0.9659), and 1415 ng/ml was the maximum cut-off value. At this point, the PH had 77.27% sensitivity and 87.5% specificity
Fig 4: Overexpression of NLRC5 enhanced cell migration and invasion of AN3CA cells. (A, B) AN3CA cells were transfected with NLRC5 plasmid and NLRC5 protein and mRNA expression was measured in AN3CA cells; **p < 0.01 vs. vector group. (C) Wound healing assay indicated that migration of AN3CA cells was upregulated after transfection with NLRC5 plasmid; **p < 0.01 vs. vector group. (D) Transwell invasion assays showed that overexpression of NLRC5 increased the invasion rate in AN3CA cells; **p < 0.01 vs. vector group. (E, F) MMP9 protein and mRNA expression was measured in AN3CA cells; **p < 0.01 vs. vector group. NLRC5, NOD-like receptor family caspase recruitment domain family domain-containing 5; AN3CA, endometrial cancer cell line; MMP9, metallopeptidase 9.
Fig 5: NLRC5 deficiency aggravates VSMC proliferation, migration, and dedifferentiation. a Human aortic smooth muscle cells (HASMCs) are transfected with scramble small interfering RNA (siCtr) or NLRC5 small interfering RNA (siNLRC5) for 48 h. HASMC proliferation is measured by MTS assay in the presence or absence of PDGF-BB (10 ng/ml) at the indicated time points. *P < 0.05 vs siNLRC5+PDGF at 36 h after PDGF-BB stimulation. b Edu incorporation (green) is evaluated by fluorescence microscopy. Hoechst 33342 is used as a nuclear stain of HASMCs. Scale bar: 100 µm. c, d After transfection with siCtr or siNLRC5 for 48 h, cell migration is assessed by scratch assay in HASMCs with or without PDGF-BB (10 ng/ml) stimulation for 12 h c. Scale bar: 100 µm. Wound area is analyzed by ImagePro Plus software d. e, f Representative western blotting of NLRC5, PCNA, Cyclin D1, a-SMA, Calponin, Myosin, and Vinculin in HASMCs transfected with siCtr or siNLRC5 in the presence of PDGF-BB for 0, 6 and 12 h (10 ng/ml). Two-tailed Student’s t-test is used to compare two groups d, and analysis of variance (ANOVA) followed by Bonferroni post hoc analysis is used to compare three or more groups a, f. Data are presented as mean ± SD from three independent experiments. *P < 0.05. Original magnification, ×100 (b and c). Source data are provided as a Source Data file
Supplier Page from Abcam for Anti-NLRC5 antibody