Fig 1: XPD inhibits the proliferation of A375 cells. Cellular proliferation was detected by MTT assay after transfecting A375 cells with pcDNA3.1(+) and pcDNA3.1(+)/XPD for 24, 48 and 72 h. Data are represented as the mean ± SD (n=3). ANOVA was used to determine significant differences among the DMEM, pcDNA3.1(+) and pcDNA3.1(+)/XPD groups. *P<0.05 compared with the control DMEM and pcDNA3.1(+) groups. XPD, xeroderma pigmentosum group D.
Fig 2: XPD is localized in the endoplasmic reticulum. (A) Immunofluorescence detection of XPD and the Golgi marker GM130, suggesting that there is no co-localization (A1, XPD; A2, GM130; A3, merge). (B) Immunofluorescence detection of XPD and the endoplasmic reticulum marker KDEL, suggesting that these 2 entities are colocalized coexist (B1, XPD; B2, KDEL; B3, merge). XPD, xeroderma pigmentosum group D.
Fig 3: Recombinant plasmids transfected into A375 cells. GFP expression in A375 cells was observed under a fluorescence microscope (magnification, ×200). (A) Negative control (A375 cells). (B) pEGFP-N1/XPD and (C) pcDNA3.1(+)/XPD transfected A375 cells. GFP, green fluorescent protein; XPD, xeroderma pigmentosum group D.
Fig 4: Detection of XPD protein expression in A375 cells transfected with pEGFP-N1/XPD and pcDNA3.1(+)/XPD. Western blot analysis Control consisted of A375 cells only. XPD, xeroderma pigmentosum group D.
Fig 5: Cloning and identification of the XPD gene. (A) Following reverse transcription from total RNA extracted from HeLa cells, nested PCR was used to obtain a 1,234 bp product (M: DNA ladder; 1: HeLa cells PCR product). (B) BglII single digestion resulted in a 4,737 and 1,200 bp product (M: DNA ladder; 1: BglII single digestion product). (C) pcDNA3.1(+)/XPD was obtained and identified by HindIII and XbaI digestion. XPD, xeroderma pigmentosum group D. (M: DNA ladder; 1: HindIII and SalI digestion products).
Supplier Page from Abcam for Anti-XPD antibody