Fig 1: Immunoblot and RT-qPCR analysis of ETFB mRNA expression. (A) 2-DE gel of spot identified as ETFB (indicated by arrow). (B) Confirmation of 2-DE results by immunoblotting. Analysis of protein expression of ETFB with ß-actin as the internal control. (C) Quantification of immunoblot result. Relative expression of ETFB protein. (D) Comparisons of total mRNA expression of ETFB by RT-qPCR. *P<0.05. 2-DE, 2-D electrophoresis; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; PUUO, partial unilateral ureteral obstruction; PUUO1d, 1 days following PUUO; PUUO2d, 2 days following PUUO; PUUO5d, 5 days following PUUO; ETFB, electron transfer flavoprotein, ß polypeptide.
Supplier Page from Abcam for Anti-ETFB antibody