Fig 1: Compound-iAs Are Functional(A) Immunostaining mouse primary cortical neurons for Map2 and synapsin after co-culturing with MEF, iAs, and mouse pAs for 5 days. The scale bar represents 50 µm.(B) Quantification of Map2+ neurite length in neurons co-cultured with MEFs, iAs, and pAs. n = 1,000 cells.(C) Increased Map2+synapsin+ puncta in neuronsco-cultured with iAs and pAs, relative to co-culture with MEFs. The scale bar represents 10 µm.(D) Quantification of Map2+synapsin+ puncta per 50-mm neurite length in neurons co-cultured with MEFs, iAs, and pAs. n = 30 neurites.(E) Measurement of glutamate uptake in iAs, pAs, and MEFs. n = 3 experimental repeats.(F) Calcium signal change in response to glutamate stimulation is shown by calcium reporter fluorescent dye intensity change (?F/F0) over time (seconds) in iAs. The scale bar represents 100 mm.(G) Lack of calcium spikes after glutamate stimulation in MEFs.(H) Calcium spikes after glutamate stimulation in pAs.(I) Quantification of ?F/F0 in MEFs, iAs, and pAs in response to glutamate stimulation. n = 400–600 cells.See also Figure S5.