Anti-GFAP antibody [2A5] ab4648 from Abcam

Product Specs
ItemAnti-GFAP antibody [2A5]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab4648
Antibody TypePrimary
HostMouse, Nude mouse
Clone2A5
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Sandwich ELISA, Western blot
ClonalityMonoclonal
IsotypeIgG1
Quantity100 UL
Conjugate/TagUnconjugated
FormatAntibody is supplied as Integra CL-350 flask material, which is concentrated tissue culture supernatant
Research AreaNeuroscience
Gene NameGFAP
ReactivityHuman, Mouse, Pig, Rat
TargetGFAP

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Dual-targeting AAV9P1-mediated neuronal reprogramming in a mouse model of traumatic brain injuryNeural Regeneration ResearchMarch 1, 2024Jingzhou Liu, Xin Xin, Jiejie Sun, Yueyue Fan, Xun Zhou, Wei Gong, Meiyan Yang, Zhiping Li, Yuli Wang, Yang Yang, et al.1:200; Abcam, Cambridge, UK, Cat# ab177487, RRID: AB_2532109), mouse anti-GFAP (1:200; Abcam, Cat# ab4648, RRID: AB_449329), rabbit anti-EGFP (1:200; Thermo Fisher Scientific, Waltham, MA, USA, Cat# CAB4211,

(2) MLKL deficiency alleviates neuroinflammation and motor deficits in the α-synuclein transgenic mouse model of Parkinson's diseaseMol NeurodegenerDecember 1, 2023Lu Geng, Wenqing Gao, Hexige Saiyin, Yuanyuan Li, Yu Zeng, Zhifei Zhang, Xue Li, Zuolong Liu, Qiang Gao, Ping An, et al.anti-Alpha-synuclein (phospho S129) (1:1000, ab51253, Abcam), rabbit anti-TH (1:400, ab75875, Abcam), mouse anti-GFAP (1:2000, ab4648, Abcam), rabbit anti-Iba1 (1:2000, ab178846, Abcam), rabbit anti-CD11b (1:5000, ab133357, Abcam), and

(3) Daphnetin alleviates neuropathic pain in chronic constrictive injury rats via regulating the NF-κB dependent CXCL1/CXCR2 signaling pathwayPharm BiolDecember 1, 2023Zhang T, Liang W, Ou W, Zhang M, Cui S, Zhang S.from the following sources: rabbit antibodies (1:200, 80788 T, CST, USA), mouse antibodies GFAP (1:50, ab4648, Abcam, UK), rabbit antibodies NF-κB (1:200, 8242 T, CST, USA), mouse antibodies NeuN (1:100, 66836

(4) Intestine Health and Barrier Function in Fattening Rabbits Fed Bovine ColostrumVeterinary SciencesNovember 15, 2023Lucia Aidos, Margherita Pallaoro, Giorgio Mirra, Valentina Serra, Marta Castrica, Stella Agradi, Giulio Curone, Daniele Vigo, Federica Riva, Claudia Maria Balzaretti, et al.treating the sections with 1:100 anti-GFAP (Glial fibrillary acidic protein monoclonal antibody aA5, ab4648, Abcam, Cambridge, UK) overnight. The sections were washed in PBS for 10 min and incubated with 10 µg/mL

(5) Astrocyte and L-lactate in the anterior cingulate cortex modulate schema memory and neuronal mitochondrial biogenesiseLifeNovember 14, 2023Mastura Akter, Mahadi Hasan, Aruna Surendran Ramkrishnan, Zafar Iqbal, Xianlin Zheng, Zhongqi Fu, Zhuogui Lei, Anwarul Karim, Ying Li107056077,Used to measure nDNA by real-time PCR Antibody,Anti-GFAP (Mouse Monoclonal),Abcam,Cat #: ab4648,1:500 (IHC) Antibody,Anti-NeuN (Rabbit Polyclonal),Merck Millipore,Cat #: AB978,1:500 (IHC) Antibody,Anti-mCherry

(6) Human cerebrospinal fluid affects chemoradiotherapy sensitivities in tumor cells from patients with glioblastomaScience AdvancesOctober 27, 2023Brett W. Stringer, Manam Inushi De Silva, Zarina Greenberg, Alejandra Noreña Puerta, Robert Adams, Bridget Milky, Michael Zabolocki, Mark van den Hurk, Lisa M. Ebert, Christine Fairly Bishop, et al.catalog no. ab5392), and anti-mouse glial fibrillary acidic protein (GFAP) (1:200; Abcam, catalog no. ab4648). Secondary antibody staining was performed with donkey anti-mouse Alexa Fluor 647 (1:250; catalog no.

(7) Knockdown of NADPH oxidase 4 reduces mitochondrial oxidative stress and neuronal pyroptosis following intracerebral hemorrhageNeural regeneration researchAugust 1, 2023Ding BY, Xie CN, Xie JY, Gao ZW, Fei XW, Hong EH, Chen WJ, Chen YZ.ab133303, RRID: AB_11155321), mouse anti-glial fibrillary acidic protein (GFAP) (1:400, Abcam, Cat# ab4648, RRID: AB_449329), rabbit anti-ionized calcium-binding adapter molecule 1 (Iba-1) (1:400, Abcam, Cat#

(8) Integrative Transcriptomic Analyses of Hippocampal-Entorhinal System Subfields Identify Key Regulators in Alzheimer's DiseaseAdvanced science (Weinheim, Baden-Wurttemberg, Germany)August 1, 2023Luo D, Li J, Liu H, Wang J, Xia Y, Qiu W, Wang N, Wang X, Wang X, Ma C, et al.The human brain sections were stained with anti‐NeuN (ab104224, Abcam, 1:200) for neurons, anti‐GFAP (ab4648, Abcam, 1:200) for astrocytes, and anti‐PSAP (10801‐1‐AP, Proteintech, 1:200). For proliferation experiments,

(9) Astrocyte-derived SerpinA3N promotes neuroinflammation and epileptic seizures by activating the NF-κB signaling pathway in mice with temporal lobe epilepsyJournal of NeuroinflammationJuly 8, 2023Chong Liu, Xue-Min Zhao, Qiao Wang, Ting-Ting Du, Mo-Xuan Zhang, Hui-Zhi Wang, Ren-Peng Li, Kun Liang, Yuan Gao, Si-Yu Zhou, et al.mouse anti-NeuN (Millipore; MAB377), rabbit anti-Olig2 (Abcam; ab109186), rabbit anti-GFAP (Abcam; ab4648), rabbit anti-IBA1 (Huabio; ET1705-78), rabbit anti-phospho-RYR2 (Ser2808) (Biorbyt; orb1093816), and

(10) 3D bio-printed living nerve-like fibers refine the ecological niche for long-distance spinal cord injury regenerationBioactive MaterialsJuly 1, 2023Jia Yang, Kaiyuan Yang, Weitao Man, Jingchuan Zheng, Zheng Cao, Chun-Yi Yang, Kunkoo Kim, Shuhui Yang, Zhaohui Hou, Guihuai Wang, et al.follows: Tuj-1 (1:500, ab18207, Abcam, UK), MAP-2 (1:100, sc-74421, Santa Cruz, USA), GFAP (1:200, ab4648, Abcam, UK). Images were taken with scanning confocal laser microscopy (LSM980 Airyscan2, Zeiss, Germany). For

(11) Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1BCell death discoveryJune 22, 2023Ke P, Gu J, Liu J, Liu Y, Tian X, Ma Y, Meng Y, Xiao F.(Proteintech, Cat No: 16972-1-AP), anti-NeuN (Millipore, NBP192693PE, Massachusetts, USA),anti-GFAP (Abcam, ab4648). The sections were washed for 3 times and incubated with secondary antibody in PBS for 1 h[DyLight

(12) First trimester human umbilical cord perivascular cells (HUCPVC) modulate the kynurenine pathway and glutamate neurotransmission in an LPS-induced mouse model of neuroinflammationJournal of InflammationMay 1, 2023Fyyaz Siddiqui, Denis Gallagher, Hannah Shuster-Hyman, Lianet Lopez, Andrée Gauthier-Fisher, Clifford L Librach(Abcam). Primary antibodies used were: Mouse monoclonal anti-GFAP antibody [2A5] (1:200, Abcam, catalog# ab4648); rabbit monoclonal EAAT2 [E3P5K] (1:50, Cell Signalling Technology, catalog# 20,848); anti-IBA1 (1:1000,

(13) Exosomal miR-128-3p reversed fibrinogen-mediated inhibition of oligodendrocyte progenitor cell differentiation and remyelination after cerebral ischemiaCNS neuroscience & therapeuticsMay 1, 2023Hou H, Wang Y, Yang L, Wang Y.against fibrinogen (1:100, Novus, NBP2‐80414), glial fibrillary acidic protein (GFAP; 1:4000, Abcam, Ab4648), ionized calcium binding adapter molecule 1 (Iba1; 1:200, Invitrogen, MA5‐27726), nestin (1:100, Invitrogen,

(14) BBB opening with focused ultrasound in nonhuman primates and Parkinson’s disease patients: Targeted AAV vector delivery and PET imagingScience AdvancesApril 21, 2023Javier Blesa, José A. Pineda-Pardo, Ken-ichi Inoue, Carmen Gasca-Salas, Tiziano Balzano, Natalia López-González Del Rey, Alejandro Reinares-Sebastián, Noelia Esteban-García, Rafael Rodríguez-Rojas, Raquel Márquez, et al.Thermo Fisher Scientific, RRID:AB_2536526; 1:1000), Iba1 (rabbit 019-19741 Wako, RRID:AB_839504; 1:1000), and GFAP (mouse Ab4648 Abcam, RRID:AB_449329; 1:2000). The sections were washed in tris-buffered saline and transferred for

(15) AMPK activation attenuates cancer-induced bone pain by reducing mitochondrial dysfunction-mediated neuroinflammationActa Biochimica et Biophysica SinicaMarch 27, 2023Heyu Yang, Yujia Wang, Shuqing Zhen, Banghua Wang, Ming Jiao, Ling Liu, Dai Li, Haili Zhu, Min Xieinhibitor Mdivi-1 (S7162) were purchased from Selleck Chemicals (Houston, USA). Anti-GFAP antibody (ab4648) was purchased from Abcam (Cambridge, UK); NLRP3 rabbit pAb (A12694), Caspase-1 rabbit pAb (A0964),

(16) Differential effects of NOX2 and NOX4 inhibition after rodent spinal cord injuryPLOS ONEMarch 10, 2023Guzal Khayrullina, Sara Bermudez, Deanna Hopkins, Young Yauger, Kimberly R. ByrnesCambridge, MA), anti-CD86 (1:250, Ab53004 Abcam), anti-iNOS (1:1000, Ab3523 Abcam), anti-GFAP (1:100, Ab4648 Abcam), and anti-CD11b (1:10,000; MCA275R Abd Serotec). Immune complexes were detected with appropriate

(17) Sinomenine alleviates diabetic peripheral neuropathic pain through inhibition of the inositol‐requiring enzyme 1 alpha–X‐box binding protein 1 pathway by downregulating prostaglandin‐endoperoxide synthase 2Journal of Diabetes InvestigationMarch 1, 2023Ji Chen, Peng Guo, Xinxin Liu, Huizhi Liao, Kemin Chen, Yuxia Wang, Jie Qin, Fengrui YangAbcam), rabbit anti‐NeuN (ab177487, 1:100; Abcam), rat anti‐glial fibrillary acidic protein (GFAP; ab4648, 1:50; Abcam), rabbit anti‐GFAP (ab7260, 1:50) and rabbit anti‐PTGS2 (ab179800, 1:50; Abcam) at 4°C

(18) Hydrogen Sulfide Inhibited Sympathetic Activation in D-Galactose-Induced Aging Rats by Upregulating Klotho and Inhibiting Inflammation in the Paraventricular NucleusBiomedicinesFebruary 15, 2023Yu H, Yu Q, Mi Y, Wang P, Jin S, Xiao L, Guo Q, Wu Y.(1:250, GTX632426, GeneTex, Irvine, CA, USA) and anti-mouse glial fibrillary acidic protein (GFAP) (1:50, ab4648, Abcam) for 12 h. The slices were washed three times with PBS before the incubation with secondary antibodies

(19) Coxsackievirus A6 Infection Causes Neurogenic Pathogenesis in a Neonatal Murine ModelVirusesFebruary 12, 2023Sun Q, Li J, Wang R, Sun T, Zong Y, Wang C, Liu Y, Li X, Song Y, Zhang Y.GTX132346, USA), mouse monoclonal anti-GFAP antibody (1:100 dilution; Abcam, Cambridge, UK, Cat No. ab4648, United Kingdom), and mouse monoclonal anti-NeuN antibody (1:100 dilution; Abcam, Cat No. ab104224,

(20) Neural stem cell-derived exosome as a nano-sized carrier for BDNF delivery to a rat model of ischemic strokeNeural regeneration researchFebruary 1, 2023Zhu ZH, Jia F, Ahmed W, Zhang GL, Wang H, Lin CQ, Chen WH, Chen LK.ab52623, RRID: AB_869991), mouse monoclonal anti-glial fibrillary acidic protein (GFAP; 1:50, Abcam, Cat# ab4648, RRID: AB_449329), and rabbit monoclonal anti-myelin oligodendrocyte glycoprotein (MOG; 1:500, Abcam,

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Anti-GFAP antibody [2A5] from Abcam

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Anti-GFAP antibody [2A5] from Abcam

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