Anti-CX3CL1 antibody ab25088 from Abcam

Product Specs
ItemAnti-CX3CL1 antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab25088
Antibody TypePrimary
Quantity100 UG
IsotypeIgG
HostRabbit
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ClonalityPolyclonal
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameCX3CL1
ReactivityHuman
TargetCX3CL1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) iRHOM2 regulates inflammation and endothelial barrier permeability via CX3CL1Experimental and Therapeutic MedicineMay 15, 2023Huiyuan Yan, Junsong Wu, Huilian Yancat. no. ab205336; Abcam), occludin (1:1,000, cat. no. ab242202; Abcam), CX3CL1 (1:1,000, cat. no. ab25088; Abcam) or GAPDH (1:1,000, cat. no. ab8245; Abcam) overnight at 4˚C. Following primary antibody incubation,

(2) Spinal Cathepsin S promotes visceral hypersensitivity via FKN/CX3CR1/p38 MAPK signaling pathwaysMolecular painJanuary 1, 2023Sun P, Lin W, Weng Y, Gong J, Huang Y, Tang Y, Lin C, Chen A, Chen Y.incubated overnight at 4°C with primary antibodies (Cat S, 1:200, sc-271619, Santa Cruz, FKN, 1:1000, ab25088, Abcam, CX3CR1, 1:1000, ab8021, Abcam) and then for 2 h at room temperature with HRP-conjugated secondary

(3) Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarctionNature communicationsDecember 10, 2022Klapproth E, Witt A, Klose P, Wiedemann J, Vavilthota N, Künzel SR, Kämmerer S, Günscht M, Sprott D, Lesche M, et al.(Vilber). Immunofluorescence To analyze the endogenous localization of ADAM10 (1:100, AB19026, Millipore) and CX3CL1 (1:100, ab25088, Abcam), heart tissue sections (mid-ventricular short axis sections, 4% PFA fixed, paraffin embedded,

(4) CX3CL1 Derived from Bone Marrow Mesenchymal Stem Cells Inhibits Aβ 1-42-Induced SH-SY5Y Cell Pathological Damage through TXNIP/NLRP3 Signaling PathwayComputational and Mathematical Methods in MedicineSeptember 8, 2022Chuan Guo, Qinxuan Li, Jiujia Xiao, Xuhui Zhou, Meichen Tian, Lei Xie, Xun Xiacultivated at 4°C with the following Ι antibodies all ordered from Abcam overnight: CX3CL1 (1 : 1000, ab25088), cleaved caspase-3 (1 : 500, ab2302), Aβ (1 : 1000, ab11132), tau (1 : 10000, ab76128), phosphorylated

(5) Polyphenolic grape stalk and coffee extracts attenuate spinal cord injury-induced neuropathic pain development in ICR-CD1 female miceScientific ReportsSeptember 2, 2022Anna Bagó-Mas, Andrea Korimová, Meritxell Deulofeu, Enrique Verdú, Núria Fiol, Viktorie Svobodová, Petr Dubový, Pere Boadas-Vaelloanti-MCP1/CCL2 (1:1000, ab25124, Abcam), rabbit anti-CCR2 (1:1000, ab203128, Abcam), rabbit anti-CX3CL1 (1:1000, ab25088, Abcam), rabbit anti-CX3CR1 (1:1000, ab8021, Abcam), rabbit anti-extracellular signal-regulated kinases

(6) SARS-CoV-2 Infection of Microglia Elicits Proinflammatory Activation and Apoptotic Cell DeathMicrobiology spectrumJune 29, 2022Jeong GU, Lyu J, Kim KD, Chung YC, Yoon GY, Lee S, Hwang I, Shin WH, Ko J, Lee JY, et al.anti-Actin (sc-47778, Santa Cruz Biotechnology), anti-Hsp70 (sc-24, Santa Cruz Biotechnology), anti-CX3CL1 (ab25088, Abcam), anti-CX3CR1 (ab8021, Abcam), anti-CD16 (80006S, Cell Signaling Technology), anti-phospho-Stat1

(7) Berberine Suppresses Leukocyte Adherence by Downregulating CX3CL1 Expression and Shedding and ADAM10 in Lipopolysaccharide-Stimulated Vascular Endothelial CellsInternational Journal of Molecular SciencesApril 27, 2022Yi-Hong Wu, Chen-Ying Wei, Wei-Chin Hong, Jong-Hwei Su Pangsulphoxide (DMSO)(D2650), and LPS were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-CX3CL1 (ab25088), anti-CX3CR1 (ab8021), and anti-ADAM10 (ab1997) antibodies were obtained from Abcam (Cambridge, UK).

(8) Expression of CX3CL1 and CCL28 in Spinal Metastases of Lung Adenocarcinoma and Their Correlation with Clinical Features and PrognosisJournal of Healthcare EngineeringApril 21, 2022Shuguang Li, Shuai Liu, Jianjun Xun, Feifei Zhaosurgery to patient death. 2.2. Immunohistochemical Staining Rabbit polyclonal antibody cx3cl1: model (ab25088) and rabbit polyclonal antibody CCL28: model (ab232837) were purchased from Abcam, Cambridge, UK. All

(9) Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathyJournal of cellular and molecular medicineFebruary 1, 2022Jiang M, Xie H, Zhang C, Wang T, Tian H, Lu L, Xu JY, Xu GT, Liu L, Zhang J.Antibody,Supplier,Catalogue#,Host,Dilution Anti‐FKN,Abcam,Ab25088,Rabbit,1:500(WB) Anti‐Iba−1,Abcam,ab178846,Rabbit,"1:1,000(WB)" Anti‐Iba−1,Woka,019–19741,Rabbit,1:500(IF) Anti‐TSPO,Abcam,Ab92291,Goat,"1:1,000(WB)

(10) Is Saffron Able to Prevent the Dysregulation of Retinal Cytokines Induced by Ocular Hypertension in Mice?Journal of Clinical MedicineOctober 20, 2021José A. Fernández-Albarral et al.[48]",1:200 GREEN,"Goat anti-rabbit Alexa Fluor 488(ref. ab150077, Abcam plc)",1:150,"Rabbit polyclonal anti-CX3CL1 (ref. ab25088, Abcam plc) [49]",1:500 GREEN,"Goat anti-rabbit Alexa Fluor 488(ref. ab150077, Abcam plc)",1:150,"Rabbit

(11) Significant higher-level C-C motif chemokine ligand 2/3 and chemotactic power in cerebral white matter than grey matter in rat and humanThe European journal of neuroscienceMarch 16, 2021Zhang J, Gong X, Xiong H.clone 2D8, Millipore), rabbit anti-CCL3 (1:800; PA1–28867, Invitrogen), and rabbit anti-CX3CL1 (1:500; Ab25088, Abcam), respectively, followed by HRP-conjugated secondary antibody (1:5,000; Jackson Immuno. Inc.).

(12) Vertebral-specific activation of the CX3CL1/ICAM-1 signaling network mediates non-small-cell lung cancer spinal metastasis by engaging tumor cell-vertebral bone marrow endothelial cell interactionsTheranosticsMarch 4, 2021Ketao Wang, Libo Jiang, Annan Hu, Chi Sun, Lei Zhou, Yiwei Huang, Qing Chen, Jian Dong, Xiaogang Zhou, Feng ZhangAntibody,Species,Target Species,Dilution Ratio,Supplier,Catelogue Number CX3CL1,Rbt,"H, M, R",1:1000,Abcam,ab25088 CX3CR1,Rbt,"H, M, R",1:1000,Abcam,ab8021 ICAM-1,Rbt,H,1:1000,Abcam,ab53013 LFA-1,Rbt,H,1:1000,CST,no.

(13) Retinal Molecular Changes Are Associated with Neuroinflammation and Loss of RGCs in an Experimental Model of GlaucomaInternational Journal of Molecular SciencesFebruary 19, 2021José A. Fernández-Albarral et al.[87]",1:200 GREEN,"Goat anti rabbit Alexa Fluor 488(ref. ab150077, Abcam plc)",1:150,"Rabbit polyclonal anti CX3CL1 (ref. ab25088, Abcam plc) [88]",1:500 GREEN,"Goat anti rabbit Alexa Fluor 488(ref. ab150077, Abcam plc)",1:150,"Rabbit

(14) Voluntary Wheel Running Reduces Vesicle Development in an Endometriosis Animal Model Through Modulation of Immune ParametersFront Reprod HealthJanuary 1, 2021Appleyard CB, Cruz ML, Velazquez-Cruz J, Rivera-Mendez RM, Jimenez-Garcia JG, Rivera LA, Del Mar Mendez-Casillas M, Flores I, Al-Nakkash L, Chompre G.sections were double labeled and incubated overnight with CD68 (Bio Rad #MCA341R; 1:50) and FKN (Abcam #ab25088; 1:1,000), CXC3R1 (Abcam #ab8021; 1:100), or ERβ (Invitrogen #PA1311; 1:500). The next day sections

(15) Upregulation of Neuronal Cylindromatosis Expression is Essential for Electroacupuncture-Mediated Alleviation of Neuroinflammatory Injury by Regulating Microglial Polarization in Rats Subjected to Focal Cerebral Ischemia/ReperfusionJournal of inflammation researchJanuary 1, 2021Lin X, Zhan J, Jiang J, Ren Y.sections were incubated at 4 °C overnight with the following primary antibodies: rabbit anti-CX3CL1 (ab25088, Abcam, United Kingdom, 1:100), mouse anti- CX3CR1 (sc-377227, Santa Cruz, United States, 1:50), rabbit

(16) STAT3/miR-15a-5p/CX3CL1 Loop Regulates Proliferation and Migration of Vascular Endothelial Cells in AtherosclerosisInternational Journal of Medical SciencesJanuary 1, 2021Hui Li et al.(ab92552) (mAbs, Abcam), rabbit Phospho-STAT3 (Ser727) (AF3294) (PcAb, Affbiotech), rabbit CX3CL1 (ab25088) (PcAb, Abcam) and mouse GAPDH (sc-51907) (mAbs, Santa Cruz). Secondary antibodies for western blot:

(17) Involvement of activated cytotoxic T lymphocytes and natural killer cells in Henoch–Schönlein purpura nephritisClinical & Translational ImmunologyNovember 24, 2020Takashi Imai, Kei Nishiyama, Kenji Ueki, Tamami Tanaka, Yoshitsugu Kaku, Toshiro Hara, Shouichi OhgaCambridge, UK), anti‐CD8 (EP1150Y, Abcam), anti‐CD56 (123C3, Invitrogen, Carlsbad, CA, USA) and anti‐CX3CL1 (ab25088, Abcam) antibodies. Antigen–antibody complexes were visualised with Histofine Simple Stain MAX PO (Nichirei

(18) The prenatal challenge with lipopolysaccharide and polyinosinic:polycytidylic acid disrupts CX3CL1-CX3CR1 and CD200-CD200R signalling in the brains of male rat offspring: a link to schizophrenia-like behavioursJournal of neuroinflammationAugust 23, 2020Chamera K, Kotarska K, Szuster-Głuszczak M, Trojan E, Skórkowska A, Pomierny B, Krzyżanowska W, Bryniarska N, Basta-Kaim A.medium was removed, and sections were immersed in appropriate primary antibody solutions (anti-CX3CL1: ab25088, 1:100; anti-CX3CR1: ab8021, 1:100; anti-OX2: ab203887, 1:100, all from Abcam, Cambridge, UK, and anti-OX2R:

(19) MicroRNA-195 prevents hippocampal microglial/macrophage polarization towards the M1 phenotype induced by chronic brain hypoperfusion through regulating CX3CL1/CX3CR1 signalingJournal of neuroinflammationAugust 20, 2020Mao M, Xu Y, Zhang XY, Yang L, An XB, Qu Y, Chai YN, Wang YR, Li TT, Ai J.fluorescent secondary antibodies (LICOR Biosciences, Lincoln, NE, USA). Anti-CX3CL1 (1:1000, Cat. #ab25088, Abcam, USA) and anti-CX3CR1 (1:1000, Cat. #ab8021, Abcam, USA) were used as the primary antibodies.

(20) Hypochlorite-induced porcine model of peritoneal fibrosis through the activation of IL1β-CX3CL1-TGFβ1 signal axisScientific reportsJuly 13, 2020Hsu YT, Wu CH, Chao CY, Wei YS, Chang YC, Chen YT, Lin SL, Tsai SY, Lee YJ, Tsai PS.(αSMA, #Ab21027), rabbit polyclonal anti-TGF-β1 (TGF-β1, #Ab92486), rabbit polyclonal anti-CX3CL1 (#Ab25088), mouse monoclonal anti- type I collagen (#Ab6308) were purchased from Abcam (Cambridge, UK). Interleukin

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Anti-CX3CL1 antibody from Abcam

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Anti-CX3CL1 antibody from Abcam

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