Anti-TIE2 antibody [Cl. 16] ab24859 from Abcam

Product Specs
ItemAnti-TIE2 antibody [Cl. 16]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab24859
Antibody TypePrimary
ApplicationsFlow Cytometry, Western blot
HostMouse, Nude mouse
Quantity100 UG
IsotypeIgG1
ClonalityMonoclonal
CloneCl. 16
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameTEK
ReactivityHuman
TargetTIE2

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Single extracellular vesicle analysis in human amniotic fluid shows evidence of phenotype alterations in preeclampsiaJournal of Extracellular VesiclesMay 18, 2022Natalia Gebara et al.manufacturer's protocol. Alexa Fluor 488 conjugated anti‐CD117 (ab2164459, Abcam, Cambridge, UK), anti‐Tie‐2 (ab24859, Abcam), anti‐CD105 (362‐820, Ancell) and HLA‐G conjugated with Alexa Fluor 555 (SC‐21799, Santa Cruz)

(2) DPSCs treated by TGF-β1 regulate angiogenic sprouting of three-dimensionally co-cultured HUVECs and DPSCs through VEGF-Ang-Tie2 signalingStem Cell Research & TherapyDecember 1, 2021Yuchen Zhang, Junqing Liu, Ting Zou, Yubingqing Qi, Baicheng Yi, Waruna Lakmal Dissanayaka, Chengfei Zhangovernight at 4 °C: anti-SM22α (#ab14106), anti-Ang1 (#ab183701), anti-VEGF-A (#ab46154), and anti-Tie2 (#ab24859), which were purchased from Abcam company (Abcam, Cambridge, UK); anti-α-SMA (#19245S), anti-p-VEGFR2

(3) Effects of remote ischemic preconditioning (RIPC) and chronic remote ischemic preconditioning (cRIPC) on levels of plasma cytokines, cell surface characteristics of monocytes and in-vitro angiogenesis: a pilot studyBasic research in cardiologyOctober 14, 2021Hummitzsch L, Zitta K, Fritze L, Monnens J, Vollertsen P, Lindner M, Rusch R, Hess K, Gruenewald M, Steinfath M, et al.room temperature. Dilutions of primary antibodies were prepared in 1% BSA (anti-Tie-2, 1:100, Abcam, ab24859, Cambridge, UK; anti-CCR2, 1:50, Novus biological, NBP1-48337, Littleton, CO, USA). Samples were incubated

(4) Deferoxamine accelerates endothelial progenitor cell senescence and compromises angiogenesisAging (Albany NY)September 15, 2021Yi-Nan Lee et al.(PECAM1, CD31; MA3105; Invitrogen), VE-cadherin (sc-9989; Santa Cruz), Flt-1 (ab2350; Abcam), TIE2 (ab24859; Abcam), eNOS (32027; Cell Signaling Technology), KDR (2479S; Cell Signaling Technology), VEGF (1909-S;

(5) Subventricular zone/white matter microglia reconstitute the empty adult microglial niche in a dynamic waveeLifeAugust 23, 2021Hohsfield LA, Najafi AR, Ghorbanian Y, Soni N, Crapser J, Figueroa Velez DX, Jiang S, Royer SE, Kim SJ, Henningfield CM, et al.Abcam), NESTIN (1:200, ab6142, Abcam), MASH1 (1:200, 556604, BD Biosciences, San Jose, CA), TIE2 (1:100, ab24859, Abcam), GFAP (1:3000, ab4674, Abcam), DCX (1:200, sc-8066, Santa Cruz, Dallas, TX), OLIG2 (1:200, ab109186,

(6) Exogenous hydrogen sulfide and miR-21 antagonism attenuates macrophage-mediated inflammation in ischemia reperfusion injury of the aged kidneyGeroScienceJune 1, 2021Pushpakumar S, Kundu S, Weber G, Sen U.Tx). iNOS was from BD Biosciences (Cat.: 610329, San Jose, CA); FSP1 (Cat.: Ab27957), Tie-2 (Cat.: Ab24859), TGF-β (Ab64715), and MST (Cat.: Ab85377) were from Abcam (Cambridge, MA). MMP-9 (Cat.: MA515886) and

(7) Resveratrol reduced the detrimental effects of malondialdehyde on human endothelial cellsJournal of Cardiovascular and Thoracic ResearchApril 24, 2021Mehdi Hassanpour, Çıgır Biray Avci, Reza Rahbarghazi, Aysa Rezabakhsh, Alireza Nourazarian, Elahe Nabat, Farzaneh Fathi, Majid Khaksardesigned by our team. A panel of antibodies against Tie-1 (Cat no: ab27851, Abcam), Tie-2 (Cat no: ab24859, Abcam), VEGFR-1 (Cat no: ab32152, Abcam), and VEGFR-2 (Cat no: ab39256, Abcam) were used. Polystyrene

(8) The roles of metastasis-related proteins in the development of giant cell tumor of bone, osteosarcoma and Ewing’s sarcomaTechnology and Health CareMarch 25, 2021Bo Dou, Tianrui Chen, Qiubo Chu, Guirong Zhang, Zhaoli Mengchemoattractant protein 3 (MCP-3) (ab130340, Abcam, Cambridge, MA, USA), Angiopoietin-1 receptor (TIE-2) (ab24859, Abcam, Cambridge, MA, USA), vascular endothelial growth factor (VEGF) (ABS82, Merck Millipore, Germany),

(9) Autophagy modulation altered differentiation capacity of CD146+ cells toward endothelial cells, pericytes, and cardiomyocytesStem Cell Research & TherapyDecember 1, 2020Mehdi Hassanpour, Jafar Rezaie, Masoud Darabi, Amirataollah Hiradfar, Reza Rahbarghazi, Mohammad NouriNo: ab32152, Abcam), VEGFR-2 (Cat No: ab39256, Abcam), Tie-1 (Cat No: ab27851, Abcam), Tie-2 (Cat No: ab24859, Abcam), and Ang-1 (Cat No: 130-06, Peprotech) by ELISA assay provided by our group [22]. The final

(10) Thymosin‑β�4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF‑κB pathwayInternational Journal of Molecular MedicineAugust 11, 2020Shumin Lv, Hongwen Cai, Yifei Xu, Jin Dai, Xiqing Rong, Lanzhi Zhengprimary antibodies against Ang2 (1:1,000; 57 kDa; cat. no. ab155106; Abcam), tie2 (1:1,000; 126 kDa; ab24859; Abcam), VEGF-A (1:1,000; 23 kDa; cat. no. ab46154; Abcam), N1ICD (1:500; 80 kDa; ab8925; Abcam), p-p65

(11) Angiopoietin-2 regulated by progesterone induces uterine vascular remodeling during pregnancyMolecular Medicine ReportsMay 27, 2020Yang-Gyu Park, Jawun Choi, Jae-Won Seol(rabbit polyclonal, Cell signaling; cat. no. 8757), and anti-Tie-2 (mouse monoclonal, Abcam; cat. no. ab24859). After several washes, the samples were incubated for 2 h at RT with the following secondary antibodies:

(12) Imaging of Tie2 with a Fluorescently Labeled Small Molecule Affinity LigandACS chemical biologyJanuary 17, 2020Koch PD, Ahmed MS, Kohler RH, Li R, Weissleder R.fixed with 4% PFA for 20 min, blocked with 3% BSA in PBS, and then stained with a Tie2 antibody (Abcam, ab24859 for anti-mouse Tie2 and ab221154 for anti-human Tie2) at 10 μg/mL for 1 hour at room temperature. Cells

(13) miR-15a/-16 Inhibit Angiogenesis by Targeting the Tie2 Coding Sequence: Therapeutic Potential of a miR-15a/16 Decoy System in Limb IschemiaMolecular Therapy - Nucleic AcidsSeptember 6, 2019Marie Besnier et al.non-ischemic and ischemic muscles was performed using a mouse anti-mouse/human Tie2 antibody (Abcam, ab24859 [Cl. 16], 1:50) and a goat anti-mouse Alexa-568 (Abcam, ab175473, 1:200) on cryosections. At least 5

(14) Integrin α5β1-Ang1/Tie2 receptor cross-talk regulates brain endothelial cell responses following cerebral ischemiaExperimental & Molecular MedicineSeptember 5, 2018Defang Pang, Lu Wang, Jing Dong, Xiaoyin Lai, Qijuan Huang, Richard Milner, Longxuan Li1:100) was obtained from Thermo Fisher Scientific (Rockford, IL, USA), and mouse anti-Tie2 antibody (AB24859, 1:25) was obtained from Abcam (Cambridge, MA, USA). The Cy3-conjugated goat anti-rat secondary antibody

(15) Overexpression of Tie2 is associated with poor prognosis in patients with gastric cancerOncology LettersMarch 23, 2018Wei‑Jun Yang, Ying‑Xue Hao, Xia Yang, Xiao‑Lan Fu, Yan Shi, Hai‑Ling Yue, Peng Yin, Hao‑Lin Dong, Pei‑Wu Yuserum albumin in PBS) at room temperature for 1 h. Primary antibodies against Tie2 (dilution, 1:50; cat no. ab24859; Abcam, Cambridge, UK) or carbonic anhydrase IX (CAIX; dilution, 1:400; cat no. ab107257; Abcam) were

(16) Hyperoxia causes miR-34a-mediated injury via angiopoietin-1 in neonatal lungsNature CommunicationsDecember 1, 2017Mansoor Syed, Pragnya Das, Aishwarya Pawar, Zubair H. Aghai, Anu Kaskinen, Zhen W. Zhuang, Namasivayam Ambalavanan, Gloria Pryhuber, Sture Andersson, Vineet Bhandari(Cell signaling; 2570; 1:500), P53 (Cell signaling; 2524; 1:2000), phospho-Tie2 (R&D; AF2720; 1:500), Tie2 (Abcam; ab24859; 1:1000), Sirt1 (Cell signaling; 2028; 1:800), cyclin D1 (Cell signaling; 2922; 1:800), phospho-c-Kit

(17) The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degenerationLaboratory investigation; a journal of technical methods and pathologyAugust 1, 2017Wang K, Liu W, Song Y, Wu X, Zhang Y, Li S, Gao Y, Tu J, Liu Y, Yang C.ice-cold lysis buffer and resuspended in SDS sample buffer for blotting analysis specific for Tie2 (ab24859), α5β1 (ab75472), anti-phosphotyrosine (ab8076) (Abcam), FAK (sc-271195), and p85 (sc-71891) (Santa Cruz Biotechnology

(18) Targeting of Beta Adrenergic Receptors Results in Therapeutic Efficacy against Models of Hemangioendothelioma and AngiosarcomaPLoS ONEMarch 28, 2013Jessica M. Stiles et al.antibodies (1∶1000, #4970)] or Abcam [p21 (1∶100, #ab7960) p-Tie2 (1∶1000, #ab788142), Tie2 (1∶1000, #ab24859)] followed with 1∶1000 HRP-conjugated secondary. Proteins were detected using Supersignal West Dura Extended Duration Substrate

(19) Variable Somatic TIE2 Mutations in Half of Sporadic Venous MalformationsMolecular SyndromologyJanuary 1, 2013J. Soblet, N. Limaye, M. Uebelhoer, L.M. Boon, M. VikkulaSanta Cruz Biotechnology) followed by stripping and reprobing with an antibody against total TIE2 (ab24859; Abcam). Proteins were visualized using goat anti-mouse-HRP (Biosource) with a femto-range-sensitive

(20) Telomerase-based immortalization modifies the angiogenic/inflammatory responses of human coronary artery endothelial cellsExperimental biology and medicine (Maywood, N.J.)June 1, 2011Yvonne Baumer, Beate Scholz, Svetlana Ivanov, Burkhard Schlosshauer

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Anti-TIE2 antibody [Cl. 16] from Abcam

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Anti-TIE2 antibody [Cl. 16] from Abcam

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