Anti-Clathrin heavy chain antibody ab21679 from Abcam

Product Specs
ItemAnti-Clathrin heavy chain antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab21679
Antibody TypePrimary
IsotypeIgG
HostRabbit
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
ClonalityPolyclonal
Quantity100 UG
Conjugate/TagUnconjugated
Concentration0.4 mg/ml
Gene NameCLTC
ReactivityHuman, Mouse, Rat
TargetClathrin heavy chain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) MARCO Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection through Intensifying Viral GP5-Induced ApoptosisMicrobiology SpectrumJune 15, 2023Xiaoxiao Zhang, Yongjie Chen, Songbei Li, Jinling Wang, Zhan He, Jiecong Yan, Xiaohong Liu, Chunhe Guoanti-PRRSV N antibody (4A5) (1:1500; Jeno Biotech, 9041), anti-clathrin heavy chain (1:1000, Abcam, ab21679), anti-Mouse IgG Alexa Fluor 488 (1:1000, Cell Signaling Technology, 4408s), and anti-Rabbit IgG Alexa

(2) Opposite changes in the expression of clathrin and caveolin-1 in normal and cancerous human prostate tissue: putative clathrin-mediated recycling of EGFRHistochemistry and Cell BiologyJune 1, 2023Boyu Xie, Hawra Zuhair, Rui Henrique, Michael Millar, Timothy Robson, Christopher Thrasivoulou, Kerry Dickens, Jane Pendjiky, Asif Muneer, Hiten Patel, et al.pathologist. Immunohistochemistry Multiplex staining of TAs, using anti-clathrin (heavy chain) (Abcam, cat. no. ab21679), anti-caveolin-1 (Abcam, cat. no. ab2910) and EGFR (Leica, NCL-EGFR) antibodies. Furthermore, anti-clathrin,

(3) An A-kinase anchoring protein (ACBD3) coordinates traffic-induced PKA activation at the GolgiThe Journal of biological chemistryMay 1, 2023Jia J, Tang S, Yue X, Jing S, Zhu L, Tan C, Gao J, Du Y, Lee I, Qian Y.(sc-393615, Santa Cruz), anti-GM130 (610822, BD Bioscience), anti-SNAP tag (P9310S, NEB), anti-Clathrin HC (ab21679, Abcam), anti-ARF1 (NB300-505, Novus), anti-β-Actin (3700S, Cell Signaling), and anti-α-Tubulin (3873S,

(4) Clathrin coats partially preassemble and subsequently bend during endocytosisJournal of Cell BiologyMarch 6, 2023Markus Mund, Aline Tschanz, Yu-Le Wu, Felix Frey, Johanna L. Mehl, Marko Kaksonen, Ori Avinoam, Ulrich S. Schwarz, Jonas Rieschain (sc-28276; Santa Cruz Biotechnology) and anti-clathrin heavy chain rabbit polyclonal antibodies (ab21679; Abcam) in 1% (w/v) BSA/PBS. After three washes with PBS, the sample was incubated for 3–4 h with a

(5) Clathrin mediates both internalization and vesicular release of triggered T cell receptor at the immunological synapseProceedings of the National Academy of Sciences of the United States of AmericaFebruary 7, 2023Kvalvaag A, Valvo S, Céspedes PF, Saliba DG, Kurz E, Korobchevskaya K, Dustin ML.#109201) as before. Primary antibodies are rabbit anti-EPN1 (Abcam, #ab75879), rabbit anti-CHC (Abcam, #ab21679), rabbit anti-HRS (Abcam, #72053), rabbit anti-TSG101 (Abcam, #125011), rabbit anti-AP2M1 (Thermo Fisher Scientific

(6) Sterolight as imaging tool to study sterol uptake, trafficking and efflux in living cellsScientific ReportsDecember 1, 2022Jarmila Králová, Martin Popr, Jan Valečka, Petr Bartůněkfrom SiR-tubulin kit (SC002) provided by Spirochrome. Antibodies Anti-clathrin heavy chain antibody (ab21679), anti-dynamin 2 antibody (ab3457) were obtained from Abcam. Caveolin-1 polyclonal antibody (PA5-17447),

(7) Differential impact of ubiquitous and muscle dynamin 2 isoforms in muscle physiology and centronuclear myopathyNat CommunNovember 11, 2022Raquel GÃ³mez-Oca, Evelina Edelweiss, Sarah Djeddi, Mathias Gerbier, XÃ¨nia Massana-MuÃ±oz, Mustapha Oulad-Abdelghani, Corinne Crucifix, Coralie Spiegelhalter, Nadia Messaddeq, Pierre Poussin-Courmontagne, et al.sequencing. Antibodies. Primary antibodies used were dysferlin (ab15108, Abcam), α-actinin (A7811, Sigma-Aldrich), CHC (ab21679, Abcam), BIN1 (clone C99D, B9428, Sigma–Aldrich), TOMM20 (ab78547, Abcam). Mouse monoclonal Myc antibody

(8) TissUExM enables quantitative ultrastructural analysis in whole vertebrate embryos by expansion microscopyCell Rep MethodsOctober 24, 2022Steib E, Tetley R, Laine RF, Norris DP, Mao Y, Vermot J.poly.",Proteintech,"Cat#11308-1-AP, Ag1848; RRID:AB_2115327" "Clathrin Heavy Chain, Rabbit poly.",Abcam,Cat#Ab21679; RRID:AB_2083165 "NUP205, Rabbit poly.",Proteintech,"Cat#24439-1-AP, Ag19832; RRID:AB_2879550" "Lamin

(9) Self-assembly and structure of a clathrin-independent AP-1:Arf1 tubular membrane coatScience AdvancesOctober 21, 2022Richard M. Hooy, Yuichiro Iwamoto, Dan A. Tudorica, Xuefeng Ren, James H. Hurleyanti-cltc [1:1000 in 0.1% Tween 20 and 0.05% milk in PBS, room temperature (RT) for 1 hour; Abcam, ab21679] and anti–glyceraldehyde-3-phosphate dehydrogenase [1:10,000 in TBS with Tween 20 (TBST), RT for 1 hour;

(10) The uncoating of EV71 in mature late endosomes requires CD-M6PRBiology OpenSeptember 15, 2022Seii Ohka et al.anti-EEA1 rabbit antibodies (1:500, ab2900, Abcam), anti-clathrin heavy chain rabbit antibodies (1:1000, ab21679, Abcam), anti-RAB5 rabbit antibodies (1:1000, ab18211, Abcam), anti-RAB7 rabbit monoclonal antibody

(11) Endocytosis in the axon initial segment maintains neuronal polarityNatureSeptember 1, 2022Eichel K, Uenaka T, Belapurkar V, Lu R, Cheng S, Pak JS, Taylor CA, Südhof TC, Malenka R, Wernig M, et al.(1:1,000), Synaptic Systems 386 003 (1:500), Synaptic Systems 386 005 (1:500)); clathrin heavy chain (Abcam ab21679 (1:500)); AP-2 complex subunit α1 (Abcam ab189995 (1:500)); α-adaptin (clone AP6, Thermo MA1-064 (1:500));

(12) Human Adenovirus Type 26 Infection Mediated by αvβ3 Integrin Is Caveolin-1-DependentMicrobiology SpectrumAugust 31, 2022Davor Nestić, Jerome Custers, Danijel Švec, Dragomira Majhencontaining DAPI for nuclei staining. The following primary antibodies were used: anti-clathrin (CLTC, ab21679, Abcam, UK), anti-caveolin-1 (CAV-1, ab2910, Abcam, UK), anti-dynamin-2 (DNM2, ab3457, Abcam, UK). Leica TCS SP8

(13) Induced nanoscale membrane curvature bypasses the essential endocytic function of clathrinJournal of Cell BiologyJuly 4, 2022Robert C. Cail, Cyna R. Shirazinejad, David G. DrubinNaCl, and 0.05% [vol/vol] Tween-20, pH 7.5). Membranes were probed with clathrin heavy chain antibody (ab21679, rabbit; Abcam) at 1:500 dilution in TBSt with 5% milk, AP2M1 antibody (ab75995, rabbit; Abcam) at 1:1,000

(14) Isotropic three-dimensional dual-color super-resolution microscopy with metal-induced energy transferScience AdvancesJune 10, 2022Jan Christoph Thiele, Marvin Jungblut, Dominic A. Helmerich, Roman Tsukanov, Anna Chizhik, Alexey I. Chizhik, Martin J. Schnermann, Markus Sauer, Oleksii Nevskyi, Jörg Enderleinab18251), and clathrin samples were incubated with rabbit anti-clathrin antibody (4 ng/μl; Abcam, no. ab21679) or mouse anti-clathrin antibody (Abcam, no. 2731) in blocking buffer for 1 hour. After primary antibody

(15) Alternative splicing of the human rhomboid family-1 gene RHBDF1 inhibits epidermal growth factor receptor activationThe Journal of biological chemistryJune 1, 2022Ji R, Shi Q, Cao Y, Zhang J, Zhao C, Zhao H, Sayyed Y, Fu L, Li LY.purchased from Cell Signaling Technology. Anti-Twist antibody (Twist2C1a), anti-clathrin heavy chain (ab21679), and anti-IRHOM1 antibody are purchased from Abcam. HSC70 polyclonal antibody, c-myc mAb, cyclin D1

(16) Exosome Mediated Cytosolic Cisplatin Delivery Through Clathrin-Independent Endocytosis and Enhanced Anti-cancer Effect via Avoiding Endosome Trapping in Cisplatin-Resistant Ovarian CancerFrontiers in MedicineMay 3, 2022Guannan Zhou et al.anti-ARF6 antibody (Abcam, ab131261), anti-Rac1 antibody (Abcam, ab155938), anti-CLTC antibody (Abcam, ab21679), anti-GAPDH (Cell signaling). Exosome Uptake Analysis Confocal microscopy was performed for detection

(17) Uric acid, as a double-edged sword, affects the activity of epidermal growth factor (EGF) on human umbilical vein endothelial cells by regulating aging processBioengineeredFebruary 14, 2022Yuan Li, Linru Zhao, Wufang Qi(catalog no. 93,443) were purchased from Sigma-Aldrich (USA). Clathrin monoclonal antibody (catalog no. ab21679, 1:500 dilution) and Caveolin monoclonal antibody (catalog no. ab87770, 1:1000 dilution) were purchased

(18) Sub-diffraction error mapping for localisation microscopy imagesNature CommunicationsDecember 1, 2021Richard J. Marsh, Ishan Costello, Mark-Alexander Gorey, Donghan Ma, Fang Huang, Mathias Gautel, Maddy Parsons, Susan Coxwere then incubated with primary antibodies (tubulin from Sigma-T8328 at 1:200, clathrin from Abcam-ab21679 at 1:500) diluted in blocking buffer for 30 min while gently rocked at RT. After three 5 min washes

(19) Direct supercritical angle localization microscopy for nanometer 3D superresolutionNature CommunicationsDecember 1, 2021Anindita Dasgupta, Joran Deschamps, Ulf Matti, Uwe Hübner, Jan Becker, Sebastian Strauss, Ralf Jungmann, Rainer Heintzmann, Jonas Riesin 100 µL 2% BSA in PBS. For primary antibody labelling, anti-clathrin heavy chain rabbit antibody (ab21679, Abcam) (1:300 final dilution) and anti-clathrin light chain rabbit antibody (sc28276, Santa Cruz Biotechnology)

(20) YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusionNature CommunicationsDecember 1, 2021Daniel Feliciano et al.(anti-Phospho-Histone H3 (Ser10); Cell Signaling, Cat. # 3377, 1:200), Anti-clathrin heavy chain (Abcam, Cat. # ab21679, 1:200), Anti-AP-2 (Abcam, Cat. # ab189995, 1:200), Anti-Glut1 (Abcam, Cat. # ab40084, 1:100), Anti-CD98

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Anti-Clathrin heavy chain antibody from Abcam

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Anti-Clathrin heavy chain antibody from Abcam

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