Anti-PCNA antibody ab18197 from Abcam

Product Specs
ItemAnti-PCNA antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab18197
Antibody TypePrimary
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
HostRabbit
ClonalityPolyclonal
IsotypeIgG
Quantity100 UG
Conjugate/TagUnconjugated
Concentration0.5 mg/ml
Gene NamePCNA
ReactivityCommon marmoset, Human, Mouse, Rat
TargetPCNA

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Hsa_circ_0028007 regulates the progression of nasopharyngeal carcinoma through the miR-1179/SQLE axisOpen Med (Wars)July 31, 2023Wenya Li, Xiuwen Jiang, Lina ZhaoMA, USA), anti-Bcl2 (1:1,000, ab692; Abcam), anti-Proliferating Cell Nuclear Antigen (PCNA; 1:1,000, ab18197; Abcam), anti-Bax (1:1,000, ab32503; Abcam), anti-Cyclin D1 (1:200, ab16663; Abcam), anti-hexokinase-2

(2) EZH2 Gene Knockdown Inhibits Sheep Pituitary Cell Proliferation via Downregulating the AKT/ERK Signaling PathwayInternational journal of molecular sciencesJune 26, 2023Cai Y, Chen P, Xu H, Li S, Zhao B, Fan Y, Wang F, Zhang Y.Band (kDa) Anti-KMT6/EZH2 antibody,ab191250,"Abcam (Boston, MA, USA)",1:5000,98 Anti-PCNA antibody,ab18197,"Abcam (MA, USA)",1:500,29 BAX rabbit polyclonal antibody,50599-2-Ig,"Protein Tech (Rosemont, IL, USA)",1:2000,24 BCL2,12789-1-AP,"Protein

(3) Improved Media Formulations for Primary Cell Cultures Derived from a Colonial UrochordateCellsJune 23, 2023Andy Qarri, Dietmar Kültz, Alison M. Gardell, Baruch Rinkevich, Yuval Rinkevichwith primary anti PCNA (Proliferating Cell Nuclear Antigen) antibody developed against rabbit (Abcam, ab18197, 1:1000), washed with 0.02% Tween-20 in PBS (2 × 10 min), and incubated with secondary antibodies (Invitrogen,

(4) Circular RNA circBNC2 facilitates glycolysis and stemness of hepatocellular carcinoma through the miR-217/high mobility group AT-hook 2 (HMGA2) axisHeliyonJune 9, 2023Yan Feng, Shufeng Xia, Junlan Hui, Yan Xuserum (5%) for 30 min, followed by the incubation with antibodies against HMGA2 (ab97276, Abcam), PCNA (ab18197, Abcam), HK2 (ab227198, Abcam) and OCT4 (ab109183, Abcam) overnight at 4 °C. Subsequently, the sections

(5) MicroRNA‐215‐5p promotes proliferation, invasion, and inhibits apoptosis in liposarcoma cells by targeting MDM2Cancer MedicineJune 1, 2023Zhengnan Song, Jingping Bai, Renbing Jiang, Junshen Wu, Wenpeng Yangcaspase‐3 (Abcam Ab32351), MDM2 (Abcam ab259265), P53 (Abcam Ab1101), P21 (Abcam Ab109520), PCNA (Abcam Ab18197), Bax (Abcam Ab32503) and Bcl‐2 (Abcam Ab182858) was detected. METHODS: 5% stacking gel and 12% separation

(6) The Role of Hedgehog Signaling in the Melanoma Tumor Bone MicroenvironmentInternational journal of molecular sciencesMay 16, 2023Shamsoon K, Hiraki D, Yoshida K, Takabatake K, Takebe H, Yokozeki K, Horie N, Fujita N, Nasrun NE, Okui T, et al.#18472-AP, rabbit IgG, Proteintech, Rosemont, IL, USA), proliferating cell nuclear antigen (PCNA) (1:1000, #ab18197, rabbit IgG, Abcam, Cambridge, MA, USA), Shh (1:500, #ab53281, rabbit IgG, Abcam), Gli1 (1:1000, #NBP1-78259,

(7) LINC00963-FOSB-mediated transcription activation of UBE3C enhances radioresistance of breast cancer cells by inducing ubiquitination-dependent protein degradation of TP73Journal of Translational MedicineMay 12, 2023Yansu Wang, Ming Liu, Xiaoqian Liu, Xianling GuoAfter that, the sections were incubated with the antibodies including KI67 (ab16667, Abcam), PCNA (ab18197, Abcam) and γH2AX (ab81299, Abcam) at 4 ℃ overnight, and then with the secondary antibody at room temperature

(8) RFWD3 promotes ZRANB3 recruitment to regulate the remodeling of stalled replication forksThe Journal of cell biologyMay 1, 2023Moore CE, Yalcindag SE, Czeladko H, Ravindranathan R, Wijesekara Hanthi Y, Levy JC, Sannino V, Schindler D, Ciccia A, Costanzo V, et al.antibodies were used: BRCA2 (A300-005A; Bethyl), FANCD2 (NB100-182; Novus), HLTF (ab183042; Abcam), PCNA (ab18197; Abcam), PCNA ubiquityl-Lys164 (13439; Cell Signaling), RFWD3 (A301-397A; Bethyl), RPA32 (A300-244A;

(9) AurkA nuclear localization is promoted by TPX2 and counteracted by protein degradationLife science allianceMay 1, 2023Asteriti IA, Polverino F, Stagni V, Sterbini V, Ascanelli C, Naso FD, Mastrangelo A, Rosa A, Paiardini A, Lindon C, et al.anti-Cep170 (final bleed, 1:100 (65)); rabbit anti-lamin B1 (ab16048, 1 µg/ml; Abcam); rabbit anti-PCNA (ab18197, 2 µg/ml; Abcam); and mouse anti-myc tag (clone 4A6, 05-724, 2 µg/ml; Merck Millipore). Fixed samples

(10) Astragaloside IV attenuates lipopolysaccharide induced liver injury by modulating Nrf2-mediated oxidative stress and NLRP3-mediated inflammationHeliyonApril 11, 2023Yuxia Sun, Yuhui Ma, Fangyuan Sun, Wentao Feng, Hui Ye, Tianning Tian, Ming Leimonoclonal, Cell Signaling Technology), GAPDH (1:1000, ab9485, rabbit polyclonal, Abcam), and PNCA (1000, ab18197, rabbit polyclonal, Abcam). HRP-conjugated secondary antibodies (1:2000) were used to detect the immune

(11) Knockdown of KDM5B Leads to DNA Damage and Cell Cycle Arrest in Granulosa Cells via MTF1Current issues in molecular biologyApril 7, 2023Yang Y, Cai Y, Guo J, Dai K, Liu L, Chen Z, Wang F, Deng M.NO.,Source,Dilution,Observed Band (kDa) Anti-KDM5B/PLU1/Jarid1B Antibody,Ab181089,Abcam,1:5000,170 Anti-PCNA Antibody,Ab18197,Abcam,1:500,29 BAX Rabbit Polyclonal Antibody,50599-2-Ig,ProteinTech,1:2000,24 Bcl-2(D17C4) Rabbit mAb

(12) O-GlcNAcylated LARP1 positively regulated by circCLNS1A facilitates hepatoblastoma progression through DKK4/β-catenin signallingClinical and translational medicineApril 1, 2023Cui Z, He J, Zhu J, Ni W, Liu L, Bian Z, Mao S, Gu S, Shan Y, Chu Z, et al.antibodies used were anti‐DKK4 (Proteintech, 27080‐1‐AP), anti‐Ki‐67 (Abcam, ab15580), anti‐PCNA (Abcam, ab18197) and anti‐β‐catenin (Abcam, ab16051). 2.6 HB orthotopic transplantation models Briefly, 4‐week‐old

(13) Circ_0001667 accelerates breast cancer proliferation and angiogenesis through regulating CXCL10 expression by sponging miR‐6838‐5pThoracic CancerApril 1, 2023Xu Zhang, Jiami Huang, Jiayun Wang, Yongheng Li, Guohua Hu, He Li(Beyotime), closed with 10% skimmed milk, and incubated with primary antibodies (Abcam) against PCNA (ab18197, 1:1000), Ki67 (ab254123, 1:5000), VEGFA (ab46154, 1:2000), FGF2 (ab208687, 1:1000), CXCL10 (ab9807,

(14) Excessive reactive oxygen species induce transcription-dependent replication stressNature communicationsMarch 30, 2023Andrs M, Stoy H, Boleslavska B, Chappidi N, Kanagaraj R, Nascakova Z, Menon S, Rao S, Oravetzova A, Dobrovolna J, et al.at 4 °C with appropriate primary antibodies diluted in 3% BSA/PBS. Both rabbit polyclonal anti-PCNA (ab18197, Abcam) and mouse monoclonal anti-RNAPII, H5 (920204, BioLegend) were used in a dilution of 1:1000.

(15) DDX47, MeCP2, and other functionally heterogeneous factors protect cells from harmful R loopsCell reportsMarch 28, 2023Marchena-Cruz E, Camino LP, Bhandari J, Silva S, Marqueta-Gracia JJ, Amdeen SA, Guillén-Mendoza C, García-Rubio ML, Calderón-Montaño JM, Xue X, et al.anti- Nucleolin,Abcam,Cat# ab50279;RRID: AB_881762 Polyclonal antibody Rabbit anti- PCNA,Abcam,Cat# ab18197;RRID: AB_444313 Monoclonal antibody Mouse anti- Phospho HistoneH2AX.X (Ser 139),Biolegend,Cat# 613402;RRID:

(16) MicroRNA-200c-5p Regulates Migration and Differentiation of Myoblasts via Targeting Adamts5 in Skeletal Muscle Regeneration and MyogenesisInternational Journal of Molecular SciencesMarch 5, 2023Yanwen Liu, Yilong Yao, Yongsheng Zhang, Chao Yan, Mingsha Yang, Zishuai Wang, Wangzhang Li, Fanqinyu Li, Wei Wang, Yalan Yang, et al.Abcam ab41037, Cambridge, UK), KI67 (1:1000, Bioss bs-23102R, Beijing, China), PCNA (1:1000, Abcam ab18197, Cambridge, UK), MyHC (developmental myosin 1:1000, DSHB MF20, Iowa, USA) and Gapdh (1:1000, Abcam ab9482,

(17) Clcf1/Crlf1a-mediated signaling is neuroprotective and required for Müller glia proliferation in the light-damaged zebrafish retinaFrontiers in Cell and Developmental BiologyFebruary 10, 2023Patrick Boyd, Leah J. Campbell, David R. Hydetemperature. Primary antibodies used were rabbit anti-PCNA (1:2000; Abcam; Cambridge, United Kingdom; AB18197) and chicken anti-GFP (1:1000; Abcam; Cambridge, United Kingdom; AB13970). Slides were washed 3 times

(18) MRL/MpJ Mice Resist to Age-Related and Long-Term Ovariectomy-Induced Bone Loss: Implications for Bone Regeneration and RepairInternational journal of molecular sciencesJanuary 25, 2023Gao X, Sun X, Cheng H, Ruzbarsky JJ, Mullen M, Huard M, Huard J.antigen retrieval was needed. For pSMAD5 (ab92698, 1:400 dilution, Abcam, Waltham, MA, USA) and PCNA (ab18197, 1:4000 dilution, Abcam, Waltham, MA, USA), heat-mediated antigen retrieval was performed using 10 mM

(19) Hsa_circ_0005050 regulated the progression of oral squamous cell carcinoma via miR-487a-3p/CHSY1 axisJournal of Dental SciencesJanuary 1, 2023Xubin Chen, Qiaojiang Chen, Chen Zhao, Zhiqi Luwhich was sealed with 5% skim milk for 2 h and then incubated overnight with primary antibodies: PCNA (ab18197, 1:1000, Abcam, Cambridge, MA, USA), CDK6 (ab151247, 1:1000, Abcam), Bcl2 (ab32124, 1:1000, Abcam),

(20) PCNA Ser46-Leu47 residues are crucial in preserving genomic integrityPloS oneJanuary 1, 2023Kim S, Kim Y, Kim Y, Yoon S, Lee KY, Lee Y, Kang S, Myung K, Oh CK.formaldehyde (P2031), and formamide (FC1014) (Biosesang). The following antibodies were used: anti-PCNA (ab18197), anti-LIG1 (ab615) (Abcam), anti-ubiquityl-PCNA (Ub-PCNA) (Lys164) (13439S), anti-histone H3 (9175),

Write a Review

(1)

8/8/2020Good Antibody for Frozen Tissue Sections Bhavin ShahE13 embryonic mouse brain tissue was stained with anti-PCNA to determine the total proliferating cells in the cortex.
(1)

3/1/2018Average Antibody For PCNA Detection Shikha SainiPCNA is a nuclear antigen that represents cellular proliferation. It is often used as a nuclear marker for Western blotting experiments especially those related to the cell cycle. We used 293T nuclear... Read Review

Anti-PCNA antibody from Abcam

Abcam

Citations (325)

Reviews

Anti-PCNA antibody from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map