Anti-PCNA antibody ab18197 from Abcam

Product Specs
ItemAnti-PCNA antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab18197
Antibody TypePrimary
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Western blot
HostRabbit
ClonalityPolyclonal
IsotypeIgG
Quantity100 UG
Conjugate/TagUnconjugated
Concentration0.5 mg/ml
Gene NamePCNA
ReactivityCommon marmoset, Human, Mouse, Rat
TargetPCNA

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Loss of Grem1-lineage chondrogenic progenitor cells causes osteoarthritisNature CommunicationsOctober 31, 2023Jia Q. Ng, Toghrul H. Jafarov, Christopher B. Little, Tongtong Wang, Abdullah M. Ali, Yan Ma, Georgette A. Radford, Laura Vrbanac, Mari Ichinose, Samuel Whittle, et al.2% BSA, 5% normal goat and 5% normal donkey serum. The following antibodies were used: anti-PCNA (#ab18197 Abcam, 1:200), ColX (#ab58632 Abcam 1:200), OCN (#ab93876 Abcam, 1:200), Lubricin/PRG4 (#ab28484 Abcam,

(2) Cerebral endothelial cell-derived extracellular vesicles regulate microglial polarization and promote autophagy via delivery of miR-672-5pCell Death & DiseaseSeptember 29, 2023Changshui Wang, Lei Feng, Li Zhu, Linlin Wu, Beibei Chen, Changmeng Cui, Mengqi Yang, Yahao Gao, Pei Jiang(ab192890, Abcam), Beclin 1 (ab62557, Abcam), SQSTM1/p62 (ab109012, Abcam), β-actin (ab8227, Abcam), PCNA (ab18197, Abcam). The secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat anti-rabbit

(3) UBC13-mediated template switching promotes replication stress resistance in FBH1-deficient cellsbioRxiv : the preprint server for biologySeptember 19, 2023Hawks AL, Bergmann A, McCraw TJ, Mason JM.phospho-RPA32 (Ser4, Ser8) polyclonal (Bethyl Laboratories, A300–245A-M, 1:1000), Rabbit anti-PCNA (Abcam, Ab18197, 1:1000), and Mouse anti-gamma H2AX (phospho S139) [9F3] (Abcam, Ab26350, 1:1000). TUNEL Assay TUNEL assay

(4) Terminal differentiation of villus tip enterocytes is governed by distinct Tgfβ superfamily membersEMBO reportsSeptember 6, 2023Linda Berková, Hassan Fazilaty, Qiutan Yang, Jan Kubovčiak, Monika Stastna, Dusan Hrckulak, Martina Vojtechova, Tosca Dalessi, Michael David Brügger, George Hausmann, et al.polyclonal)",Abcam,ab15580 "Muc2 (mouse, monoclonal)",Santa Cruz,sc‐15334 "Pcna (rabbit, polyclonal)",Abcam,ab18197 "S6rp (rabbit, monoclonal)",Cell Signalling,2217 Oligonucleotides and other sequence‐based reagents,Oligonucleotides

(5) β-Catenin and SOX2 Interaction Regulate Visual Experience-Dependent Cell Homeostasis in the Developing Xenopus ThalamusInternational Journal of Molecular SciencesSeptember 2, 2023Juanmei Gao, Yufang Lu, Yuhao Luo, Xinyi Duan, Peiyao Chen, Xinyu Zhang, Xiaohua Wu, Mengsheng Qiu, Wanhua ShenNkx2.2,"DSHB (Iowa City, IA, USA)",74.5A5,AB_531794,1:50 (IF) "PCNA, rabbit",Recombinant human PCNA,Abcam,ab18197,AB_444313,1:200 (IF) Phospho-β-catenin,"Residues surrounding Ser33, Ser37 and Thr41 of human β-catenin",CST,9561,AB_331729,1:1000

(6) Hsa_circ_0028007 regulates the progression of nasopharyngeal carcinoma through the miR-1179/SQLE axisOpen Med (Wars)July 31, 2023Wenya Li, Xiuwen Jiang, Lina ZhaoMA, USA), anti-Bcl2 (1:1,000, ab692; Abcam), anti-Proliferating Cell Nuclear Antigen (PCNA; 1:1,000, ab18197; Abcam), anti-Bax (1:1,000, ab32503; Abcam), anti-Cyclin D1 (1:200, ab16663; Abcam), anti-hexokinase-2

(7) Actin nucleators safeguard replication forks by limiting nascent strand degradationNucleic Acids ResearchJuly 7, 2023Jadwiga Nieminuszczy, Peter R Martin, Ronan Broderick, Joanna Krwawicz, Alexandra Kanellou, Camelia Mocanu, Vicky Bousgouni, Charlotte Smith, Kuo-Kuang Wen, Beth L Woodward, et al.(Santa Cruz, sc-5300, 1:500), N-WASp (Abcam, ab187527, 1:500), PCNA (Santa Cruz, sc-56, 1:500), PCNA (Abcam, ab18197, 1:500) and RAD51 (Sigma Aldrich, PC130). Images were acquired using a Zeiss Axio Observer Z1 Marianas™

(8) EZH2 Gene Knockdown Inhibits Sheep Pituitary Cell Proliferation via Downregulating the AKT/ERK Signaling PathwayInternational journal of molecular sciencesJune 26, 2023Cai Y, Chen P, Xu H, Li S, Zhao B, Fan Y, Wang F, Zhang Y.Band (kDa) Anti-KMT6/EZH2 antibody,ab191250,"Abcam (Boston, MA, USA)",1:5000,98 Anti-PCNA antibody,ab18197,"Abcam (MA, USA)",1:500,29 BAX rabbit polyclonal antibody,50599-2-Ig,"Protein Tech (Rosemont, IL, USA)",1:2000,24 BCL2,12789-1-AP,"Protein

(9) Improved Media Formulations for Primary Cell Cultures Derived from a Colonial UrochordateCellsJune 23, 2023Andy Qarri, Dietmar Kültz, Alison M. Gardell, Baruch Rinkevich, Yuval Rinkevichwith primary anti PCNA (Proliferating Cell Nuclear Antigen) antibody developed against rabbit (Abcam, ab18197, 1:1000), washed with 0.02% Tween-20 in PBS (2 × 10 min), and incubated with secondary antibodies (Invitrogen,

(10) Circular RNA circBNC2 facilitates glycolysis and stemness of hepatocellular carcinoma through the miR-217/high mobility group AT-hook 2 (HMGA2) axisHeliyonJune 9, 2023Yan Feng, Shufeng Xia, Junlan Hui, Yan Xuserum (5%) for 30 min, followed by the incubation with antibodies against HMGA2 (ab97276, Abcam), PCNA (ab18197, Abcam), HK2 (ab227198, Abcam) and OCT4 (ab109183, Abcam) overnight at 4 °C. Subsequently, the sections

(11) MicroRNA‐215‐5p promotes proliferation, invasion, and inhibits apoptosis in liposarcoma cells by targeting MDM2Cancer MedicineJune 1, 2023Zhengnan Song, Jingping Bai, Renbing Jiang, Junshen Wu, Wenpeng Yangcaspase‐3 (Abcam Ab32351), MDM2 (Abcam ab259265), P53 (Abcam Ab1101), P21 (Abcam Ab109520), PCNA (Abcam Ab18197), Bax (Abcam Ab32503) and Bcl‐2 (Abcam Ab182858) was detected. METHODS: 5% stacking gel and 12% separation

(12) SETD4 Confers Cancer Stem Cell Chemoresistance in Nonsmall Cell Lung Cancer Patients via the Epigenetic Regulation of Cellular QuiescenceStem Cells InternationalMay 27, 2023Yuehong Wang, Yuman Yu, Weijun Yang, Linying Wu, Yaoshun Yang, Qianyun Lu, Jianying ZhouKi67 (Cell Signaling Technology; 9449S for IF assays and Abcam; ab16667 for WB assays), PCNA (Abcam; ab18197), H4K20me3 (Cell Signaling Technology; 5737S), H3K9ac (Abcam; ab10812), HP1-α (Cell Signaling Technology;

(13) The Role of Hedgehog Signaling in the Melanoma Tumor Bone MicroenvironmentInternational journal of molecular sciencesMay 16, 2023Shamsoon K, Hiraki D, Yoshida K, Takabatake K, Takebe H, Yokozeki K, Horie N, Fujita N, Nasrun NE, Okui T, et al.#18472-AP, rabbit IgG, Proteintech, Rosemont, IL, USA), proliferating cell nuclear antigen (PCNA) (1:1000, #ab18197, rabbit IgG, Abcam, Cambridge, MA, USA), Shh (1:500, #ab53281, rabbit IgG, Abcam), Gli1 (1:1000, #NBP1-78259,

(14) LINC00963-FOSB-mediated transcription activation of UBE3C enhances radioresistance of breast cancer cells by inducing ubiquitination-dependent protein degradation of TP73Journal of Translational MedicineMay 12, 2023Yansu Wang, Ming Liu, Xiaoqian Liu, Xianling GuoAfter that, the sections were incubated with the antibodies including KI67 (ab16667, Abcam), PCNA (ab18197, Abcam) and γH2AX (ab81299, Abcam) at 4 ℃ overnight, and then with the secondary antibody at room temperature

(15) AurkA nuclear localization is promoted by TPX2 and counteracted by protein degradationLife science allianceMay 1, 2023Asteriti IA, Polverino F, Stagni V, Sterbini V, Ascanelli C, Naso FD, Mastrangelo A, Rosa A, Paiardini A, Lindon C, et al.anti-Cep170 (final bleed, 1:100 (65)); rabbit anti-lamin B1 (ab16048, 1 µg/ml; Abcam); rabbit anti-PCNA (ab18197, 2 µg/ml; Abcam); and mouse anti-myc tag (clone 4A6, 05-724, 2 µg/ml; Merck Millipore). Fixed samples

(16) RFWD3 promotes ZRANB3 recruitment to regulate the remodeling of stalled replication forksThe Journal of cell biologyMay 1, 2023Moore CE, Yalcindag SE, Czeladko H, Ravindranathan R, Wijesekara Hanthi Y, Levy JC, Sannino V, Schindler D, Ciccia A, Costanzo V, et al.antibodies were used: BRCA2 (A300-005A; Bethyl), FANCD2 (NB100-182; Novus), HLTF (ab183042; Abcam), PCNA (ab18197; Abcam), PCNA ubiquityl-Lys164 (13439; Cell Signaling), RFWD3 (A301-397A; Bethyl), RPA32 (A300-244A;

(17) Astragaloside IV attenuates lipopolysaccharide induced liver injury by modulating Nrf2-mediated oxidative stress and NLRP3-mediated inflammationHeliyonApril 11, 2023Yuxia Sun, Yuhui Ma, Fangyuan Sun, Wentao Feng, Hui Ye, Tianning Tian, Ming Leimonoclonal, Cell Signaling Technology), GAPDH (1:1000, ab9485, rabbit polyclonal, Abcam), and PNCA (1000, ab18197, rabbit polyclonal, Abcam). HRP-conjugated secondary antibodies (1:2000) were used to detect the immune

(18) Knockdown of KDM5B Leads to DNA Damage and Cell Cycle Arrest in Granulosa Cells via MTF1Current issues in molecular biologyApril 7, 2023Yang Y, Cai Y, Guo J, Dai K, Liu L, Chen Z, Wang F, Deng M.NO.,Source,Dilution,Observed Band (kDa) Anti-KDM5B/PLU1/Jarid1B Antibody,Ab181089,Abcam,1:5000,170 Anti-PCNA Antibody,Ab18197,Abcam,1:500,29 BAX Rabbit Polyclonal Antibody,50599-2-Ig,ProteinTech,1:2000,24 Bcl-2(D17C4) Rabbit mAb

(19) Circ_0001667 accelerates breast cancer proliferation and angiogenesis through regulating CXCL10 expression by sponging miR‐6838‐5pThoracic CancerApril 1, 2023Xu Zhang, Jiami Huang, Jiayun Wang, Yongheng Li, Guohua Hu, He Li(Beyotime), closed with 10% skimmed milk, and incubated with primary antibodies (Abcam) against PCNA (ab18197, 1:1000), Ki67 (ab254123, 1:5000), VEGFA (ab46154, 1:2000), FGF2 (ab208687, 1:1000), CXCL10 (ab9807,

(20) O-GlcNAcylated LARP1 positively regulated by circCLNS1A facilitates hepatoblastoma progression through DKK4/β-catenin signallingClinical and translational medicineApril 1, 2023Cui Z, He J, Zhu J, Ni W, Liu L, Bian Z, Mao S, Gu S, Shan Y, Chu Z, et al.antibodies used were anti‐DKK4 (Proteintech, 27080‐1‐AP), anti‐Ki‐67 (Abcam, ab15580), anti‐PCNA (Abcam, ab18197) and anti‐β‐catenin (Abcam, ab16051). 2.6 HB orthotopic transplantation models Briefly, 4‐week‐old

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(1)

8/8/2020Good Antibody for Frozen Tissue Sections Bhavin ShahE13 embryonic mouse brain tissue was stained with anti-PCNA to determine the total proliferating cells in the cortex.
(1)

3/1/2018Average Antibody For PCNA Detection Shikha SainiPCNA is a nuclear antigen that represents cellular proliferation. It is often used as a nuclear marker for Western blotting experiments especially those related to the cell cycle. We used 293T nuclear... Read Review

Anti-PCNA antibody from Abcam

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Anti-PCNA antibody from Abcam

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