Anti-HA tag antibody [HA.C5] ab18181 from Abcam

Product Specs
ItemAnti-HA tag antibody [HA.C5]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab18181
Antibody TypePrimary
HostMouse, Nude mouse
CloneHA.C5
Quantity100 UG
ClonalityMonoclonal
IsotypeIgG3
ApplicationsImmunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameHA
ReactivitySpecies independent
TargetHA tag

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) The TRIM69-MST2 signaling axis regulates centrosome dynamics and chromosome segregationNucleic Acids ResearchOctober 27, 2023Yilin Wang, Patrik Risteski, Yang Yang, Huan Chen, Gaith Droby, Andrea Walens, Deepika Jayaprakash, Melissa Troester, Laura Herring, Jonathan Chernoff, et al.antibody—ChIP Grade (Abcam, ab9110), Mouse Anti-HA tag Monoclonal Antibody, Unconjugated, Clone HA.C5 (Abcam, ab18181), c-Myc antibody [9E10] - ChIP Grade (Abcam, ab32), Nuclear Pore Complex Proteins antibody [Mab414]—ChIP

(2) Pharmacological inhibition of BAP1 recruits HERC2 to competitively dissociate BRCA1–BARD1, suppresses DNA repair and sensitizes CRC to radiotherapyActa Pharmaceutica Sinica BAugust 1, 2023Xin Yue, Tingyu Liu, Xuecen Wang, Weijian Wu, Gesi Wen, Yang Yi, Jiaxin Wu, Ziyang Wang, Weixiang Zhan, Ruirui Wu, et al.MA, USA); antibody to β-tubulin (No. T0023; WB 1:1000) from Affinity Biologicals; antibody to HA (No. ab18181; WB 1:1000; IP 1:1000), RAD51 (No. ab63801; WB 1:1000; IF 1:200) from Abcam; BAP1 (No. sc-28283; WB

(3) The Piezo channel is central to the mechano-sensitive channel complex in the mammalian inner earResearch SquareJuly 13, 2023Jeong-Han Lee, Cristina Perez-Flores, Seojin Park, Hyo Jeong Kim, Yingying Chen, Mincheol Kang, Jennifer Kersigo, Jinsil Choi, Phung N. Thai, Ryan Woltz, et al.(EX-Mm02159-M56) and mouse Cib2 (CS-Mm07673-M02–01J). Antibodies were as follows: anti-HA (Abcam, cat no. ab9110, ab18181), anti-His (Abcam, cat no. ab18184, ab9108), anti-Myc (Abcam, cat no. ab32, ab9106), anti-mCherry (Abcam,

(4) Human atlastin-3 is a constitutive ER membrane fusion catalystThe Journal of cell biologyJuly 3, 2023Bryce S, Stolzer M, Crosby D, Yang R, Durand D, Lee TH.HA transfections, cells were fixed as described above and then stained with mouse anti-HA antibody (ab18181; 1:500; Abcam) in blocking solution (PBS containing 0.2 M glycine, 2.5% calf serum, and 0.1% Triton

(5) The Effect of Outer Space and Other Environmental Cues on Bacterial ConjugationMicrobiology SpectrumJune 15, 2023Bar Piscon, Eliana Pia Esposito, Boris Fichtman, Guy Samburski, Lihi Efremushkin, Shimon Amselem, Amnon Harel, Galia Rahav, Raffaele Zarrilli, Ohad Gal-MorLaboratories) as we previously described (66). Blots were probed with anti-HA tag antibody (Abcam; ab18181, diluted 1: 1,000). Goat anti-mouse antibody conjugated to horseradish peroxidase (Abcam; ab6789, diluted

(6) Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) familyNature communicationsMay 30, 2023Deshmukh FK, Ben-Nissan G, Olshina MA, Füzesi-Levi MG, Polkinghorn C, Arkind G, Leushkin Y, Fainer I, Fleishman SJ, Tawfik D, et al.used for Western blots include anti-HA(R) rabbit (1:6000, ab9110, Abcam), anti-HA(M) mouse (1:1000, ab18181, Abcam), anti-PSMD1 (1:1000, ab2941, Abcam), anti-PSMD2 (1:1000, PAB6715, Abnova), anti-PSMA3 (1:500,

(7) Insights into the expanding intestinal phenotypic spectrum of SOCS1 haploinsufficiency and therapeutic optionsJournal of clinical immunologyMay 9, 2023Rodari MM, Cazals-Hatem D, Uzzan M, Martin Silva N, Khiat A, Ta MC, Lhermitte L, Touzart A, Hanein S, Rouillon C, et al.for 1 h, membranes were incubated with anti-SOCS1 (A156, #3950 Cell Signaling), anti-HA tag (HA.C5, ab18181, Abcam), anti-STAT1 (9H2, #9176, Cell Signaling), anti-pSTAT-1 (58D6, #9167, Cell Signaling), anti-STAT5

(8) HY5 functions as a systemic signal by integrating BRC1-dependent hormone signaling in tomato bud outgrowthProceedings of the National Academy of SciencesApril 18, 2023Han Dong, Jiachun Wang, Xuewei Song, Chaoyi Hu, Changan Zhu, Ting Sun, Zhiwen Zhou, Zhangjian Hu, Xiaojian Xia, Jie Zhou, et al.plants. Transgenic plants were identified by western blot using an anti-HA monoclonal antibody (Abcam, ab18181). Two independent homozygous lines of the F2 progeny of pBRC1–LOG4 and pBRC1–CKX7 transgenic plants

(9) The plant unique ESCRT component FREE1 regulates autophagosome closureNature CommunicationsMarch 30, 2023Yonglun Zeng, Baiying Li, Shuxian Huang, Hongbo Li, Wenhan Cao, Yixuan Chen, Guoyong Liu, Zhenping Li, Chao Yang, Lei Feng, et al.anti-ATG8 (Agrisera, AS142769), anti-GFP (Chromotek, 029762), anti-RFP (Abcam, ab125244), anti-HA (Abcam, ab18181), anti-MYC (Santa Cruz, SC789), and anti-SnRK1α1 (Agrisera, AS10919). In vivo phosphorylation assay In

(10) CircVPRBP inhibits nodal metastasis of cervical cancer by impeding RACK1 O-GlcNAcylation and stabilityOncogeneMarch 10, 2023Chunyu Zhang, Hongye Jiang, Li Yuan, Yuandong Liao, Pan Liu, Qiqiao Du, Chaoyun Pan, Tianyu Liu, Jie Li, Yili Chen, et al.Abcam), mouse anti-flag antibody (1:5000, Cat#F3165, Sigma-Aldrich), or rabbit anti-HA (1:1000, Cat# ab18181, Abcam). RNA immunoprecipitation (RIP) RIP assay was performed by Magna RIPTM RNA-binding protein

(11) PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinomaNature communicationsFebruary 23, 2023Wang K, Luo L, Fu S, Wang M, Wang Z, Dong L, Wu X, Dai L, Peng Y, Shen G, et al.1:2000; ab32146, 1:5000), PRMT1 (ab190892, 1:1000 for immunoblotting, and 1:100 for IHC staining), HA (ab18181, 1:4000), and Ki-67 (ab16667, 1:500) were purchased from Abcam. Antibodies against PHGDH (PA5-27578,

(12) Linking medicinal cannabis to autotaxin–lysophosphatidic acid signalingLife Science AllianceFebruary 1, 2023Mathias C Eymery, Andrew A McCarthy, Jens HausmannHampton Research; and InstantBlue Coomassie protein stain (#ab119211), anti-HA tag primary antibody (#ab18181), anti-mouse antibody (#ab150113), anti-ATX antibody (#ab77104), anti-mouse HRP secondary antibody (#ab6728),

(13) Klf4-Sirt3/Pparα-Lcad pathway contributes to high phosphate-induced lipid degradationCell communication and signaling : CCSJanuary 9, 2023Yu A, Xu Y, Hogstrand C, Zhao T, Tan XY, Wei X, Song YF, Luo Z.anti-LCAD (Proteintech, 17526-1-AP, USA), anti-PPARα (Proteintech, 15540-1-AP), anti-HA tag (Abcam, ab18181), anti-Flag tag (Abcam, ab1162), anti-GAPDH (CST, 2118) and anti-acetyl lysine (Abcam, ab21623). After

(14) A NR2E1-interacting peptide of LSD1 inhibits the proliferation of brain tumour initiating cellsCell proliferationJanuary 1, 2023Hu R, Hameed UFS, Sun X, Moorthy BS, Zhang W, Jeffrey PD, Zhou L, Ma X, Chen F, Pei J, et al.plasmids. Anti‐NR2E1 (ab30942, Abcam), anti‐LSD1 (ab17721, Abcam), anti‐FLAG (#F1804, SIGMA) and anti‐HA (ab18181, Abcam) antibodies were used to pull down protein complexes. Immunoprecipitated complexes, bound to

(15) Direct attenuation of Arabidopsis ERECTA signalling by a pair of U-box E3 ligasesNature plantsJanuary 1, 2023Chen L, Cochran AM, Waite JM, Shirasu K, Bemis SM, Torii KU.transferred to PVDF membrane (Millipore) for immunoblot analysis using anti-HA (1:1,000; Abcam, catalogue no. ab18181) and anti-MYC (1:1,000; Abcam, catalogue no. ab32) as primary antibodies. As secondary antibody, goat anti-mouse IgG H&L (HRP

(16) Stabilization of MCL-1 by E3 ligase TRAF4 confers radioresistanceCell Death & DiseaseDecember 19, 2022Ming Li, Feng Gao, Xiaoying Li, Yu Gan, Shuangze Han, Xinfang Yu, Haidan Liu, Wei LiThe antibodies against DUB3 (#ab112436, 1:1000), JOSD1 (#ab118221, IB: 1:1000, IP: 1:200), HA tag (#ab18181, 1:5000) and His tag (#ab18184, 1:5000) were purchased from Abcam (Cambridge, UK). Plasmid construction Flag-TRAF4

(17) Targeting ferroptosis with miR-144-3p to attenuate pancreatic β cells dysfunction via regulating USP22/SIRT1 in type 2 diabetesDiabetology & Metabolic SyndromeDecember 1, 2022Shanshan Zhang, Xiao Liu, Jihong Wang, Fengjuan Yuan, Yali LiuFor immunoprecipitation, the cell lysate was incubated with anti-flag (#ab1162, Abcam) or anti-HA (#ab18181, Abcam) or anti-Myc (#ab32) antibody and Dynabeads Protein G at 4 °C overnight. Then, the beads were

(18) An assembly of nuclear bodies associates with the active VSG expression site in African trypanosomesNature CommunicationsDecember 1, 2022James Budzak, Robert Jones, Christian Tschudi, Nikolay G. Kolev, Gloria Rudenkoantibodies used were from Abcam: anti-mCherry (ab167453, 1:2000), anti-myc (ab9106, 1:1000) and anti-HA (ab18181, 1:750). The highly SUMOylated focus was visualised using an anti-SUMO antibody (kind gift of the Miguel

(19) Architecture of the outbred brown fat proteome defines regulators of metabolic physiologyCellNovember 23, 2022Xiao H, Bozi LHM, Sun Y, Riley CL, Philip VM, Chen M, Li J, Zhang T, Mills EL, Emont MP, et al.5% FBS) for 10 min at room temperature. For immunofluorescence staining, anti-HA mouse mAb (Abcam #ab18181) at 1:100 and anti-TOMM20 rabbit mAb (Cell Signaling #42406) or anti-COX4 rabbit mAb (Cell Signaling

(20) Ubiquitin receptors play redundant roles in the proteasomal degradation of the p53 repressor MDM2FEBS lettersNovember 1, 2022Sparks A, Kelly CJ, Saville MK.Proteintech Group (Rosemont, IL, USA); ADRM1 (ab56852), RAD23A (EPR4817, ab108591), RAD23B (ab3835), HA tag (HA.C5, ab18181), NOXA (114C307, ab13654) and beta‐actin (ab8226), Abcam (Cambridge, UK); Ubiquitin (P4D1‐A11, 05‐944),

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Anti-HA tag antibody [HA.C5] from Abcam

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Anti-HA tag antibody [HA.C5] from Abcam

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