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Antibodies
Anti-GFAP antibody
Anti-GFAP antibody from Abcam
Be the first to write a review!
Citations:
(48)
Supplier Page
Supplier Page from
Abcam for
Anti-GFAP antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-GFAP antibody
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab16997
Antibody Type
Primary
Clonality
Polyclonal
Applications
Immunocytochemistry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Host
Rabbit
Quantity
1 ml
Isotype
IgG
Conjugate/Tag
Unconjugated
Concentration
0.1 mg/ml
Research Area
Neuroscience
Gene Name
GFAP
Reactivity
Human, Mouse
Target
GFAP
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (48)
(1)
Neuron-binding antibody responses are associated with Black ethnicity in multiple sclerosis during natalizumab treatment
Brain Commun
August 14, 2023
Kiel M Telesford, Chad Smith, Marcel Mettlen, Melissa B Davis, Lindsay Cowell, Rick Kittles, Timothy Vartanian, Nancy Monson
antibodies in blocking buffer at 4°C overnight: 1/100 Rabbit anti-glial fibrillary acidic protein (GFAP
ab16997
,
Abcam
), 1/200
Mouse
anti-
MAP2
(
MA5–12826
,
Invitrogen
), and 10 µg/mL
purified human IgG
. The next day,
(2)
Severity- and Time-Dependent Activation of Microglia in Spinal Cord Injury
International Journal of Molecular Sciences
May 5, 2023
Elvira Ruslanovna Akhmetzyanova, Margarita Nikolaevna Zhuravleva, Anna Viktorovna Timofeeva, Leisan Gazinurovna Tazetdinova, Ekaterina Evgenevna Garanina, Albert Anatolevich Rizvanov, Yana Olegovna Mukhamedshina
cells were fixed in 4% buffered formalin, washed with 0.1% Triton X-100 in PBS and stained with GFAP (
ab16997
,
Abcam
, Cambridge, UK), Iba1 (
ab5076
,
Abcam
), TNF-α (
ab199013
,
Abcam
) and TGF-β (
ab92486
,
Abcam
) antibodies
(3)
Young Astrocytic Mitochondria Attenuate the Elevated Level of CCL11 in the Aged Mice, Contributing to Cognitive Function Improvement
International Journal of Molecular Sciences
March 8, 2023
Ryosuke Tashiro, Dan Ozaki, Jesus Bautista-Garrido, Guanghua Sun, Lidiya Obertas, Alexis S. Mobley, Gab Seok Kim, Jaroslaw Aronowski, Joo Eun Jung
were incubated with primary antibodies against glial fibrillary acidic protein (GFAP) (
1:200
dilution,
ab16997
,
Abcam
), vimentin (
1:200
dilution,
ab92547
,
Abcam
), and aldehyde dehydrogenase 1 family member L1 (ALDH1L1)
(4)
Astrocytic cell adhesion genes linked to schizophrenia correlate with synaptic programs in neurons
Cell reports
January 31, 2023
Pietiläinen O, Trehan A, Meyer D, Mitchell J, Tegtmeyer M, Valakh V, Gebre H, Chen T, Vartiainen E, Farhi SL,
et al
.
(
1:10,000
,
Abcam
ab5392
), Rabbit anti-Syn-1 (
1:1000
millipore AB1543P), Rabbit anti-GFAP (
1:250
,
Abcam
ab16997
), Rabbit anti-Cx43 (
1:500
,
ab230537
).
Goat
Alexafluor plus-555- conjugated anti-
mouse
(Cat # A32727)
(5)
Tissue libraries enable rapid determination of conditions that preserve antibody labeling in cleared mouse and human tissue
eLife
January 19, 2023
Theodore J Zwang, Rachel E Bennett, Maria Lysandrou, Benjamin Woost, Anqi Zhang, Charles M Lieber, Douglas S Richardson, Bradley T Hyman
specifications:
NeuN
antibody conjugated to Alexa Fluor 568 (
Abcam
,
Ab207282
).
GFAP
antibody unconjugated (
Abcam
,
ab16997
) and conjugated to Alexa Fluor 488 (
Thermo Fisher
,
53-9892-82
).Glut1 antibody conjugated to Alexa Fluor
(6)
Motoneuronal inflammasome activation triggers excessive neuroinflammation and impedes regeneration after sciatic nerve injury
Journal of neuroinflammation
March 19, 2022
Molnár K, Nógrádi B, Kristóf R, Mészáros Á, Pajer K, Siklós L, Nógrádi A, Wilhelm I, Krizbai IA.
BA-1000
, RRID:AB_2313606,
Vector Laboratories
)" GFAP (
IHC
),"Polyclonal rabbit against GFAP,
1:500
(Cat#
ab16997
, RRID:AB_443592,
Abcam
, Cambridge, UK)","Biotinylated goat anti-rabbit IgG Antibody (H + L),
1:500
(Cat#
(7)
Complementary encoding of spatial information in hippocampal astrocytes
PLOS Biology
March 3, 2022
Sebastiano Curreli, Jacopo Bonato, Sara Romanzi, Stefano Panzeri, Tommaso Fellin
Saint Louis, Missouri, USA) and coverslipped. Primary antibodies were Anti-GFAP (
1:300
rabbit,
Abcam
Ab16997
, Cambridge, United Kingdom), Anti-NeuN (
1:250
mouse
,
Millipore
MAB377
, Billerica, Massachusetts, USA),
(8)
Met-RANTES preserves the blood-brain barrier through inhibiting CCR1/SRC/Rac1 pathway after intracerebral hemorrhage in mice
Fluids and barriers of the CNS
January 21, 2022
Yan J, Xu W, Lenahan C, Huang L, Ocak U, Wen J, Li G, He W, Le C, Zhang JH,
et al
.
(
Abcam
, MA, USA) were used as follows: rabbit anti-CCR1 (
1:200
, PA1-41062), rabbit anti-GFAP (
1:200
,
ab16997
), rabbit anti-NeuN (
1:200
,
ab177487
) and rabbit anti-vWF (
1:200
,
ab6994
). Lastly, the slices were incubated
(9)
Traumatic brain injury results in unique microglial and astrocyte transcriptomes enriched for type I interferon response
Journal of Neuroinflammation
December 1, 2021
Brittany P. Todd, Michael S. Chimenti, Zili Luo, Polly J. Ferguson, Alexander G. Bassuk, Elizabeth A. Newell
temperature. After blocking, tissues were incubated overnight at 4 °C in rabbit anti-GFAP (
1:100
;
Abcam
AB16997
) or rabbit anti-Iba1 (
1:200
; Wako Chemicals 019-17941) primary antibody diluted in blocking/extraction
(10)
Prostaglandin EP2 receptor antagonist ameliorates neuroinflammation in a two-hit mouse model of Alzheimer’s disease
Journal of Neuroinflammation
December 1, 2021
Avijit Banik, Radhika Amaradhi, Daniel Lee, Michael Sau, Wenyi Wang, Raymond Dingledine, Thota Ganesh
hippocampus using a microtome (Leica). For
immunofluorescent staining
, rabbit polyclonal to GFAP (
Abcam
,
ab16997
,
1:500
dilution) and Iba1 (
Wako
,
019-19741
,
1:500
dilution) primary antibodies were used. For secondary
(11)
Synthetic amyloid-β oligomers drive early pathological progression of Alzheimer's disease in nonhuman primates
iScience
October 22, 2021
Yue F, Feng S, Lu C, Zhang T, Tao G, Liu J, Yue C, Jing N.
AB_223652 Rabbit anti-NF-H,
Proteintech
,Cat #
21471-1-AP
; RRID: AB_10734324 Rabbit anti-GFAP,
Abcam
,Cat #
ab16997
; RRID: AB_443592 Rabbit anti-Iba1,
Wako
,Cat #
019-19741
; RRID: AB_839504 Goat anti-Iba1,
Abcam
,Cat #
(12)
BAD-mediated neuronal apoptosis and neuroinflammation contribute to Alzheimer's disease pathology
iScience
September 24, 2021
Zhang L, Qian Y, Li J, Zhou X, Xu H, Yan J, Xiang J, Yuan X, Sun B, Sisodia SS,
et al
.
ab32445
; RRID:AB_725614 Mouse anti-COX-IV,
Abcam
,Cat#
ab33985
; RRID:AB_879754 Rabbit anti-GFAP,
Abcam
,Cat#
ab16997
; RRID:AB_443592 Rabbit anti-
CD68
,
Abcam
,Cat#
ab125212
; RRID:AB_10975465 Rabbit anti-BDNF,
Abcam
,Cat#
(13)
Transplantation of tauroursodeoxycholic acid–inducing M2‐phenotype macrophages promotes an anti‐neuroinflammatory effect and functional recovery after spinal cord injury in rats
Cell Proliferation
June 1, 2021
Gong Ho Han, Seong Jun Kim, Wan‐Kyu Ko, Daye Lee, In‐Bo Han, Seung Hun Sheen, Je Beom Hong, Seil Sohn
Cat.
ab8918
; Abcam) and polyclonal anti‐rabbit glial fibrillary acidic protein (GFAP) (
1:200
; Cat.
ab16997
;
Abcam
). The slides were then stained with fluorescent secondary Alexa 488 (Cat.
R37114
;
Invitrogen
)
(14)
Recombinant CCL17-dependent CCR4 activation alleviates neuroinflammation and neuronal apoptosis through the PI3K/AKT/Foxo1 signaling pathway after ICH in mice
Journal of neuroinflammation
March 1, 2021
Deng S, Jin P, Sherchan P, Liu S, Cui Y, Huang L, Zhang JH, Gong Y, Tang J.
primary antibodies at 4 °C overnight: anti-Iba-1 (
1:100
,
ab178847
,
Abcam
, MA, USA); antiGFAP (
1:200
,
ab16997
,
Abcam
); anti-
NeuN
(
1:200
,
ab177487
,
Abcam
); anti-CCR4 (
1:100
, Santa Cruz Biotechnology, USA); anti-myeloperoxidase
(15)
Anti-aging effects of Ribes meyeri anthocyanins on neural stem cells and aging mice
Aging
September 12, 2020
Gao J, Wu Y, He D, Zhu X, Li H, Liu H, Liu H.
Cat No:
ab15580
), TuJ1 (Covance, Princeton, NJ, USA; Cat No: MMS-435P-250), and
GFAP
(
Abcam
, Cat No:
ab16997
) overnight at 4°C. The next day, slides were washed three times and incubated with appropriate Alexa
(16)
Deficiency of anti-inflammatory cytokine IL-4 leads to neural hyperexcitability and aggravates cerebral ischemia-reperfusion injury
Acta pharmaceutica Sinica. B
September 1, 2020
Chen X, Zhang J, Song Y, Yang P, Yang Y, Huang Z, Wang K.
cortical
neurons
, we also used rabbit anti-GFAP (glial fibrillary acidic protein) antibody (
1:200
,
Abcam
,
ab16997
) for
astrocytes
; recombinant anti-
Iba1
(ionized calcium-binding adaptor molecule-1) antibody [EPR16589]
(17)
The effects of the Panax Vietnamensis ethanol fraction on proliferation and differentiation of mouse neural stem cells
Biomedical Research and Therapy
October 28, 2019
Huy Quang Do, Nhung Hai Truong, Thanh Thai Lam, Linh Thuy Nguyen, Dung Minh Le, Nhung Hong-Thi Dinh, Luan Cong Tran, Phuong Thi-Bich Le, Ngoc Kim Phan, Phuc Van Pham
FCM fixation buffer at RT for 5 minutes before being incubated with 1st rabbit anti-mouse GFAP antibody (
1:100
ab16997
,
Abcam
, Singapore) and rhodamineconjugated 2nd anti-Rb antibody (4 µ g/mL #
31670
ThermoFisher Scientific
,
(18)
Delayed recanalization at 3 days after permanent MCAO attenuates neuronal apoptosis through FGF21/FGFR1/PI3K/Caspase-3 pathway in rats
Experimental neurology
October 1, 2019
Zheng W, Matei N, Pang J, Luo X, Song Z, Tang J, Zhang JH.
h, followed by incubating in different primary antibodies at 4 °C overnight: mouse anti-GFAP (
1:150
,
ab16997
,
Abcam
, Cambridge, MA, USA),
mouse
anti-neuronal nuclei (
NeuN
,
1:200
,
ab177487
,
Abcam
, Cambridge, MA,
(19)
Astrogliosis inhibition attenuates hydrocephalus by increasing cerebrospinal fluid reabsorption through the glymphatic system after germinal matrix hemorrhage
Experimental Neurology
October 1, 2019
Yan Ding, Tongyu Zhang, Guangyong Wu, Devin W. McBride, Ningbo Xu, Damon W. Klebe, Yiting Zhang, Qian Li, Jiping Tang, John H. Zhang
Laboratories) and visualized using a microscope (Leica). Primary antibodies were anti-GFAP (
Abcam
,
ab16997
), anti-AQP4 (
Santa Cruz Biotechnology
,
sc-9888
), and anti-RECA-1 (
Santa Cruz Biotechnology
,
sc-52665
).
(20)
Prosaposin is a biomarker of mesenchymal glioblastoma and regulates mesenchymal transition through the TGF-β1/Smad signaling pathway
The Journal of pathology
September 1, 2019
Jiang Y, Zhou J, Hou D, Luo P, Gao H, Ma Y, Chen YS, Li L, Zou D, Zhang H,
et al
.
article is protected by copyright. All rights reserved. differentiation capacity markers, GFAP (#
ab16997
,
1:100
,
Abcam
) and ² III tubulin (#
ab18207
,
1:100
,
Abcam
). The molecular subtype of the cells was determined according
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Anti-GFAP antibody from Abcam
Supplier Page
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