Fig 2: Expression of apoptotic markers in T98G cells following RB starvation, PDT exposition and GW4869 administration scheme. Caspase 3 (CASP3, revealed by anti-Caspase 3 antibody, Cell Signaling, #9662), PARP1 (Cell Signaling, #9532) and a-tubulin (TUBA1A, Cell Signaling, #2144) proteins expression in untreated standard growing (DMEM) or HBSS starvation, with/without RB (0.75 µM), GW4869 (GW = 5 µM), PDT stimulation (2 min at 562/576 nm), after additional 24 h p.t. in standard growing conditions. For each sample, 40 µg of total protein extracts was loaded into 12% polyacrylamide gels. All the experiments were performed in duplicates with independent assays. A representative experiment is shown. Molecular weights in KDa are reported.