Anti-Choline Acetyltransferase antibody ab18736 from Abcam

Product Specs
ItemAnti-Choline Acetyltransferase antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab18736
Antibody TypePrimary
ClonalityPolyclonal
ApplicationsImmunocytochemistry
HostSheep
Quantity100 UG
IsotypeIgG
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaNeuroscience
Gene NameCHAT
ReactivityPig, Rat
TargetCholine Acetyltransferase

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) ENGRAILED‐1 transcription factor has a paracrine neurotrophic activity on adult spinal α‐motoneuronsEMBO reportsAugust 3, 2023Mélanie Lebœuf, Stephanie E Vargas‐Abonce, Eugénie Pezé‐Hedsieck, Edmond Dupont, Lucia Jimenez‐Alonso, Kenneth L Moya, Alain ProchiantzReagent or resource,Source,Identifier Antibodies,Antibodies,Antibodies Sheep anti‐ChAT,ABCAM,ab18736; RRID:AB_2244867 Goat anti‐ChAT,Millipore,AB‐144P; RRID:AB_2079751 Rabbit anti‐EN1 86/8,Home made,Sonnier

(2) Spinal cord tissue engineering using human primary neural progenitor cells and astrocytesBioengineering & translational medicineMarch 1, 2023Jin C, Wu Y, Zhang H, Xu B, Liu W, Ji C, Li P, Chen Z, Chen B, Li J, et al.,R&D Systems,AF3075,Goat,1:200 P2Y12,GeneTex,GTX54796,Rabbit,1:1000 Iba1,Abcam,ab15690,Mouse,1:500 GFP,Abcam,ab290,Rabbit,1:500 GFP,GeneTex,GTX13970,Chicken,1:500 vGLUT1,Millipore,AB5905,Guinea Pig,1:300 GABA,MilliporeSigma,A0310,Mouse,1:250 ChAT,Abcam,ab18736,Sheep,1:250 PSD95,Abcam,ab18258,Rabbit,1:200 SYN,Millipore,MAB329,Mouse,1:200 5‐HT,ImmunoStar,"20,080",Rabbit,1:200 CS56,Abcam,ab11570,Mouse,1:500 CD68,Abcam,ab125212,Rabbi,1:500 RECA,Abcam,ab22492,Mouse,1:500 Antibodies

(3) TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult miceActa pharmacologica SinicaJanuary 1, 2023Shi CJ, Lian JJ, Zhang BW, Cha JX, Hua QH, Pi XP, Hou YJ, Xie X, Zhang R.ab11426), rabbit anti-Calretinin (1:200; Abcam, Cat# ab16694), rabbit anti-ChAT (1:200; Abcam, Cat# ab18736), rabbit anti-nNOS (1:200; Invitrogen, Cat# PA1-033), and rabbit anti-TH (1:200; Abcam, Cat# ab6211).

(4) Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic MiceInternational Neurourology JournalSeptember 30, 2022Mi-Hye Kwon, Min-Ji Choi, Fang-Yuan Liu, Fitri Rahma Fridayana, Lashkari Niloofar, Guo Nan Yin, Ji-Kan Ryutyrosine hydroxylase (TH, Abcam; 1:50; Cat# ab112), choline acetyltransferase (ChAT, Abcam; 1:50; Cat# ab18736), calcitoningene related peptide (CGRP, Abcam; 1:50; Cat# ab36001), and βIII-tubulin (a neuronal marker;

(5) Spinal Muscular Atrophy autophagy profile is tissue-dependent: differential regulation between muscle and motoneuronsActa Neuropathologica CommunicationsDecember 1, 2021Alba Sansa, Ivan Hidalgo, Maria P. Miralles, Sandra de la Fuente, M. Jose Perez-Garcia, Francina Munell, Rosa M. Soler, Ana Garceraanti-Laminin2, 1:75, Cat. No. L0663, Sigma; anti-HB9, 1:75, Cat. No. ab92606; anti-ChAT, 1:100, Cat. No. ab18736, both from Abcam; or anti-Islet1/2, 1:50, Cat. No. 39.4D5, Developmental Studies Hybridoma Bank) was

(6) Laterodorsal tegmentumâ€“ventral tegmental area projections encode positive reinforcement signalsJournal of Neuroscience ResearchNovember 1, 2021BÃ¡rbara Coimbra, Ana VerÃ³nica Domingues, Carina Soaresâ€Cunha, Raquel Correia, LuÃsa Pinto, Nuno Sousa, Ana JoÃ£o Rodriguesanti‐c‐fos (1:1,000, Ab‐5, Millipore), goat anti‐ChAT (1:750, AB144P, Millipore), sheep anti‐ChAT (1:750, ab18736, Abcam), mouse anti‐dopamine D1 receptor (1:300, sc‐33660, Santa Cruz Biotechnology), mouse anti‐dopamine

(7) Identification of new enterosynes using prebiotics: roles of bioactive lipids and mu-opioid receptor signalling in humans and miceGutJune 1, 2021Anne Abot et al.incubated with goat anti-nNOS (1/100, ab1376, Abcam), sheep anti-ChAT (Choline Acetyl Transferase, 1/100, ab18736, Abcam), rabbit anti-MOR (1/100, ab10275, Abcam) or rabbit anti-Alox12 (1/100, bs3874R, Bioss) primary

(8) Top-down acetylcholine signaling via olfactory bulb vasopressin cells contributes to social discrimination in ratsCommunications biologyMay 21, 2021Suyama H, Egger V, Lukas M.Cell Signaling Technology, Frankfurt am Main, Germany), Sheep Anti-Choline Acetyltransferase (1:125, ab18736, Abcam, Berlin, Germany). Secondary antibodies were Donkey anti-Goat IgG conjugated with CF488 (for

(9) Intracellular G-actin targeting of peripheral sensory neurons by the multifunctional engineered protein C2C confers relief from inflammatory painScientific ReportsDecember 1, 2020Derek Allen, You Zhou, Audrey Wilhelm, Paul BlumMotor neurons were identified using antibodies against choline acetyltransferase (ChAT, Abcam, Cat # ab18736) with a 1:300 d ilution39. Coverslips were then mounted on slides using fluorogel mounting solution

(10) Inhibition of ER stress improves progressive motor deficits in a REEP1-null mouse model of hereditary spastic paraplegiaBiology OpenSeptember 15, 2020Bingjie Wang, You Yu, Lai Wei, Yan Zhangantibody (Santa Cruz Biotechnology, sc-17750), Bip antibody (CST, 3177), choline acetyltransferase (Abcam, ab18736), REEP1 antibody (Sigma-Aldrich, SAB2101976), and NeuN antibody (Abcam, ab177487) were used for immunofluorescence.

(11) Calpain system is altered in survival motor neuron-reduced cells from in vitro and in vivo spinal muscular atrophy modelsCell death & diseaseJune 25, 2020de la Fuente S, Sansa A, Hidalgo I, Vivancos N, Romero-Guevara R, Garcera A, Soler RM.No. ab92606; anti-Calpastatin antibody, 1:100, Cat. No. ab28252; anti-ChAT antibody, 1:100, Cat. No. ab18736, all from Abcam; anti-Mu-Calpain antibody, 1:100, Cat. No. 9A4H8D3, Invitrogen) was diluted in 0.01%

(12) A Simple and Efficient Method for Concomitant Isolation and Culture of Enriched Astroglial and Microglial Cells from the Rat Spinal CordBIO-PROTOCOLJanuary 20, 2020Pooja Shree Mishra, Trichur R Raju(BSA) (Sigma-Aldrich, catalog number: A2153) 3. Anti-ChAT (rabbit polyclonal Abcam, catalog number: ab18736; 1:500) 4. Anti-GFAP (mouse monoclonal, Abcam, catalog number: ab24345; 1:500) 5. Anti-IBA1 (Rabbit

(13) Acetylcholine Receptor Activation as a Modulator of Glioblastoma InvasionCellsOctober 5, 2019Emily G. Thompson, Harald SontheimerPrimary antibody mouse anti-MMP9 (Abcam, ab119906, Cambridge, United Kingdom) and sheep anti-ChAT (Abcam ab18736) were diluted 1:100 in blocking solution and applied at room temperature for 1 h. The coverslips were

(14) Role of laterodorsal tegmentum projections to nucleus accumbens in reward-related behaviorsNature communicationsSeptember 12, 2019Coimbra B, Soares-Cunha C, Vasconcelos NAP, Domingues AV, Borges S, Sousa N, Rodrigues AJ.anti-EAAC1 (1:500, MAB1587, Millipore), goat anti-ChAT (1:750, AB144P, Millipore) or sheep anti-ChAT (1:500, ab18736, Abcam) followed by appropriate secondary fluorescent antibodies (Alexafluor, 1:1000, Invitrogen, MA,

(15) Effects of Resveratrol on the Mechanisms of Antioxidants and Estrogen in Alzheimer’s DiseaseBioMed Research InternationalMarch 20, 2019Danli Kong, Yan Yan, Xiao-Yi He, Huihuang Yang, BiYu Liang, Jin Wang, Yuqing He, Yuanlin Ding, Haibing Yuanti-ERβ antibody (Santa Cruz Biotechnology, Item No. SC-390243), and anti-ChAT antibody (Abcam, Item No. ab18736) were also purchased. SOD, CAT, and MDA primers were all designed and synthesized by TaKaRa. An anti-HO-1

(16) A novel role for the extracellular matrix glycoprotein-Tenascin-X in gastric functionThe Journal of physiologyMarch 1, 2019Aktar R, Peiris M, Fikree A, Eaton S, Kritas S, Kentish SJ, Araujo EJA, Bacarin C, Page AJ, Voermans NC, et al.and mouse specific stomach regions. Additionally, choline acetyl transferase (ChAT) (dilution 1:400, ab18736, Abcam) was used in brainstem sections. Immunohistochemistry process Tissue was fixed in 4% paraformaldehyde

(17) The Y172 Monoclonal Antibody Against p-c-Jun (Ser63) Is a Marker of the Postsynaptic Compartment of C-Type Cholinergic Afferent Synapses on MotoneuronsFrontiers in cellular neuroscienceJanuary 1, 2019Gatius A, Tarabal O, Cayuela P, Casanovas A, Piedrafita L, Salvany S, Hernández S, Soler RM, Esquerda JE, Calderó J.CA, USA; cat.# 73-014); sheep polyclonal anti-choline acetyltransferase (ChAT, 1:1,000; Abcam cat.# Ab18736); rabbit polyclonal anti-ChAT (1:200; Millipore, Burlington, MA, USA; cat.# AB143); rabbit polyclonal

(18) Nanosensors for the Chemical Imaging of Acetylcholine Using Magnetic Resonance ImagingACS NanoJune 26, 2018Yi Luo, Eric H. Kim, Chris A. Flask, Heather A. Clarkfollowed by serum-blocking buffer for 60 min at room temperature. Sheep polyclonal anti-ChAT antibody (ab18736, Abcam) diluted 1:1,000 in immunobuffer containing 1% normal rabbit serum (16120107, Thermo Fisher)

(19) Loss of Displaced Starburst Amacrine Cells in a Rat Glaucoma ModelJournal of Ophthalmic Research and Ocular CareApril 14, 2017Cooley-Themm CA, Ameel Q, Linn DM, Linn CLOphthalmic Res Ocular Care 2017, 1(1):28-38 gen 554892), sheep anti rat-ChAT (1:5000, 0.5 mg/mL, ABCAM Ab18736), or rabbit anti-rat cleaved caspase-3 (Asp175) (1:300, 10 mM, Cell Signaling Technology #9661) overnight

(20) Hypocretinergic and cholinergic contributions to sleep-wake disturbances in a mouse model of traumatic brain injuryNeurobiology of sleep and circadian rhythmsJanuary 1, 2017Thomasy HE, Febinger HY, Ringgold KM, Gemma C, Opp MR.protocols (Schmidt and Grady, 1995): free-floating sections in a 1:3 series were processed for ChAT (Abcam; ab18736; 1:2000 dilution). Sections were incubated in biotinylated secondary antibody (Abcam; ab97123; 1:500

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Anti-Choline Acetyltransferase antibody from Abcam

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Anti-Choline Acetyltransferase antibody from Abcam

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