Anti-KDM1/LSD1 antibody - Nuclear Marker ab17721 from Abcam

Product Specs
ItemAnti-KDM1/LSD1 antibody - Nuclear Marker
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab17721
Antibody TypePrimary
ClonalityPolyclonal
HostRabbit
Quantity100 UG
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
IsotypeIgG
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaEpigenetics
Gene NameKDM1A
ValidationKnockOut Validated
ReactivityHuman, Mouse, Rat
TargetKDM1 / LSD1

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(1) JMJD6–BRD4 complex stimulates lncRNA HOTAIR transcription by binding to the promoter region of HOTAIR and induces radioresistance in liver cancer stem cellsJournal of Translational MedicineOctober 25, 2023Ruifeng Pei, Le Zhao, Yiren Ding, Zhan Su, Deqiang Li, Shuo Zhu, Lu Xu, Wei Zhao, Wuyuan Zhoumembrane was incubated with diluted primary antibody [JMJD6 (1:1000, ab64575, Abcam), LSD1 (1:1200, ab17721, Abcam), ERK2 (MAPK1) (1:1000, 9926, CST) and BRD4 (1:1500, ab75898, Abcam)] at 4 °C overnight. HRP-conjugated

(2) "Nuclear translocation of an aminoacyl-tRNA synthetase may mediate a chronic ""integrated stress response"""Cell reportsJune 27, 2023Jones JA, Wei N, Cui H, Shi Y, Fu G, Rauniyar N, Shapiro R, Morodomi Y, Berenst N, Dumitru CD, et al.RRID:AB_1269482 P66a,Novus Biologicals,Catalog #: NB100-56643; RRID:AB_2294492 LSD1,Abcam,Catalog #: ab17721; RRID:AB_443964 TRIM28,Abcam,Catalog #: ab10483; RRID:AB_297222 Caspase 3,Cell Signaling Technology,Catalog

(3) SIRT1 ubiquitination is regulated by opposing activities of APC/C-Cdh1 and AROS during stress-induced premature senescenceExperimental & Molecular MedicineJune 1, 2023Sang Hyup Lee, Ji-Hye Yang, Ui-Hyun Park, Hanbyeul Choi, Yoo Sung Kim, Bo-Eun Yoon, Hye-Jeong Han, Hyun-Taek Kim, Soo-Jong Um, Eun-Joo KimMerck Millipore, 05-904), Myc (Merck Millipore, 05-724), Flag M2 (Sigma‒Aldrich, F1804), LSD1 (Abcam, ab17721), H3K9me2 (Abcam, ab1120), H3K9ac (Abcam, ab12179), α-SMA-Cy3 (Sigma, C6198), collagen type I (Abcam,

(4) LSD1 Inhibition Disrupts Super-Enhancer–Driven Oncogenic Transcriptional Programs in Castration-Resistant Prostate CancerCancer ResearchMay 15, 2023Muqing Li, Mingyu Liu, Wanting Han, Zifeng Wang, Dong Han, Susan Patalano, Jill A. Macoska, Steven P. Balk, Housheng Hansen He, Eva Corey, et al.(Thermo Fisher Scientific), followed by a brief sonication, and then the lysates were immunoprecipitated with anti-LSD1 (ab17721, Abcam), anti-BRD4 (A301–985A100, Bethyl Laboratories), or anti-FOXA1 (ab23738, Abcam). For immunoblotting,

(5) Pan-cancer analysis revealed H3K4me1 at bivalent promoters premarks DNA hypermethylation during tumor development and identified the regulatory role of DNA methylation in relation to histone modificationsBMC GenomicsMay 4, 2023Yang Lu, Qiang Cao, Yue Yu, Yazhou Sun, Xuan Jiang, Xin LiChIP: H3K4me1 (Abcam, ab8895), H3K4me3 (Abcam, ab8580), H3K27me3 (Active motif, 39055), LSD1 (Abcam, ab17721), IgG (Abcam, ab150157). ChIP-seq libraries were constructed and sequenced by the Novogene Bioinformatics

(6) Nutrient regulation of the islet epigenome controls adaptive insulin secretionJournal of Clinical InvestigationApril 17, 2023Matthew Wortham, Fenfen Liu, Austin R. Harrington, Johanna Y. Fleischman, Martina Wallace, Francesca Mulas, Medhavi Mallick, Nicholas K. Vinckier, Benjamin R. Cross, Joshua Chiou, et al.and ChIP was performed as described (67) with 30 μg of sheared chromatin and 4 μg anti-Lsd1 antibody (ab17721, Abcam) in a total volume of 240 μL. For histone ChIP, islets were processed as described previously

(7) LSD1/PRMT6-targeting gene therapy to attenuate androgen receptor toxic gain-of-function ameliorates spinobulbar muscular atrophy phenotypes in flies and miceNature communicationsFebruary 6, 2023Prakasam R, Bonadiman A, Andreotti R, Zuccaro E, Dalfovo D, Marchioretti C, Tripathy D, Petris G, Anderson EN, Migazzi A, et al.antibodies: AR (H280, Santa Cruz, #sc-13062, 1:500), PRMT6 (Santa Cruz, #sc-55702, 1:500), and LSD1 (Abcam, #ab17721, 1:500). Secondary antibodies [Invitrogen (1:500): Donkey anti-Goat 647 (#A-21447), Donkey anti-Rat

(8) LSD1 defines the fiber type-selective responsiveness to environmental stress in skeletal muscleeLifeJanuary 25, 2023Hirotaka Araki, Shinjiro Hino, Kotaro Anan, Kanji Kuribayashi, Kan Etoh, Daiki Seko, Ryuta Takase, Kensaku Kohrogi, Yuko Hino, Yusuke Ono, et al.co-IP, immunohistochemistry, and immunocytochemistry experiments were as follows: anti-LSD1 (Abcam, ab17721), anti-FOXK1 (Abcam, ab18196), anti-ERRγ (Abcam, ab12893), anti-MHC type I (Developmental Studies Hybridoma

(9) A NR2E1-interacting peptide of LSD1 inhibits the proliferation of brain tumour initiating cellsCell proliferationJanuary 1, 2023Hu R, Hameed UFS, Sun X, Moorthy BS, Zhang W, Jeffrey PD, Zhou L, Ma X, Chen F, Pei J, et al.from 200 to 600 bp and immunoprecipitated using the anti‐NR2E1 (a gift from Liu's lab), anti‐LSD1 (ab17721, Abcam), anti‐H3K4me (ab8895, Abcam), anti‐H3K4me2 (ab7766, Abcam) antibodies and control IgG or control

(10) Anti-cancer effect of LINC00478 in bladder cancer correlates with KDM1A-dependent MMP9 demethylationCell Death DiscoveryDecember 1, 2022Han-Jie Yang, Tian Liu, Yang Xionganalyzed by qPCR detection. The antibody used in RIP was rabbit anti KDM1A (dilution ratio of 1:100, ab17721, Abcam) and IgG (dilution ratio of 1:100, ab109489, Abcam) as NC [32]. RNA pull-down assay Bladder cancer

(11) Aberrant expression of KDM1A inhibits ferroptosis of lung cancer cells through up-regulating c-MycScientific reportsNovember 10, 2022Lu C, Cai Y, Liu W, Peng B, Liang Q, Yan Y, Liang D, Xu Z.antibodies at 4 °C overnight. The following antibodies were used: anti-KDM1A (Abcam, United States, Cat#ab17721), anti-c-Myc (Santa Cruz, United States,Cat# sc-40), anti-HMOX1 (Proteintech, United States, Cat# 10701-1-AP)

(12) Histone lysine demethylase inhibition reprograms prostate cancer metabolism and mechanicsMolecular MetabolismOctober 1, 2022Ugo Chianese et al.(ab9502), caspase 8 (ab9746), BCL2 (ab28725), p-eNOS (ab184154), eNOS (ab5589), p-AKT (ab81283) and LSD1 (ab17721) from Abcam. Fatty Acid and Lipid Metabolism Antibody Sampler Kit (#8335), GLUT1 (D3J3 A) and GAPDH

(13) Fine-tuned KDM1A alternative splicing regulates human cardiomyogenesis through an enzymatic-independent mechanismiScienceJuly 15, 2022Astro V, Ramirez-Calderon G, Pennucci R, Caroli J, Saera-Vila A, Cardona-Londoño K, Forastieri C, Fiacco E, Maksoud F, Alowaysi M, et al.RRID:AB_2536771 Rabbit anti-NANOG,Abcam,Cat#ab109250; RRID:AB_10863442 Rabbit anti-KDM1A,Abcam,Cat#ab17721; RRID:AB_443964 Rabbit anti-KDM1A,Abcam,Cat#ab129195; RRID:AB_11145494 Mouse anti-KDM1A,Abcam,Cat#ab31954;

(14) The biguanide polyamine analog verlindamycin promotes differentiation in neuroblastoma via induction of antizymeCancer Gene TherapyJuly 1, 2022Zuzanna Urban-Wójciuk et al.glyceraldehyde 3-phosphate dehydrogenase (GAPDH) 2118L, Aurora A 4718, Cyclin D1 2922; Abcam: ODC1 ab97395, LSD1 ab17721) at 1:1000 for 2 h at room temperature. After washing, the membrane was incubated with secondary antibody

(15) The epilepsy-associated protein PCDH19 undergoes NMDA receptor-dependent proteolytic cleavage and regulates the expression of immediate-early genesCell reportsMay 24, 2022Gerosa L, Mazzoleni S, Rusconi F, Longaretti A, Lewerissa E, Pelucchi S, Murru L, Giannelli SG, Broccoli V, Marcello E, et al.tubulin(clone B-5-1-2),Sigma-Aldrich,Cat# T5168; RRID: AB_477579 Rabbit polyclonal anti-LSD1,Abcam,Cat# ab17721; RRID: AB_443964 Rabbit polyclonal anti-CoREST,Millipore,Cat# 07-455; RRID: AB_310629 Rabbit monoclonal

(16) Targeting lysine‐specific demethylase 1A inhibits renal epithelial–mesenchymal transition and attenuates renal fibrosisThe FASEB JournalMay 23, 2022Xiaoqin Zhang, Linda Xiaoyan Li, Chen Yu, Karl A. Nath, Shougang Zhuang, Xiaogang Lico-staining, after antigen retrieval, tissue sections were incubated with a rabbit anti-mouse LSD1 antibody (ab17721; Abcam) and anti-alpha-smooth muscle actin antibody (14–9760-82; Sigma) overnight, and then were incubated

(17) LINC00586 Represses ASXL1 Expression Thus Inducing Epithelial-To-Mesenchymal Transition of Colorectal Cancer Cells Through LSD1-Mediated H3K4me2 DemethylationFrontiers in PharmacologyMay 2, 2022Fengting Liu, Xiaofang Ma, Xiyun Bian, Chunyan Zhang, Xiaozhi Liu, Qiang Liurecommendation. In brief, two parts of cell extracts were incubated with either anti-LSD1 antibody (ab17721, Abcam) or nonspecific rabbit IgG (ab2410, Abcam) and immunoprecipitated with magnetic Protein A/G beads.

(18) A NR2E1-Interacting Peptide of LSD1 Inhibits the Proliferation of Brain Tumor Initiating CellsSSRN Electronic JournalApril 3, 2022Rong Hu et al.in TBS+0.1% Tween at cold room overnight. The primary antibodies and dilutions were: rabbit antiLSD1 (ab17721, Abcam) at 1/1000, rabbit anti-NR2E1 (a gift from Haikun Liu’s lab) at 1/500, rabbit antiPTEN ( ab-31392,

(19) Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatinNature communicationsMarch 23, 2022Rivera C, Lee HG, Lappala A, Wang D, Noches V, Olivares-Costa M, Sjöberg-Herrera M, Lee JT, Andrés ME.(BD Biosciences, #612146, IF: 1:100), Rabbit anti-RCOR1 (Abcam, ab183711, IP: 2 µg) rabbit anti-LSD1/KDM1 (Abcam, ab17721, WB: 1:1000, IF: 1:500, IP: 2 µg); rabbit anti-HDAC1 (Abcam, ab7028, WB: 1:5000, IF: 1:1000, IP: 2 µg);

(20) YAP signaling in horizontal basal cells promotes the regeneration of olfactory epithelium after injuryStem cell reportsMarch 8, 2022Wu Q, Xu X, Miao X, Bao X, Li X, Xiang L, Wang W, Du S, Lu Y, Wang X, et al.Abcam), anti-PH3 (1:500, ab14955, Abcam), anti-Sox9 (1:500, ab185966, Abcam), and anti-LSD1 (1:200, ab17721, Abcam). The sections were washed three times with PBS and incubated with the corresponding secondary

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Anti-KDM1/LSD1 antibody - Nuclear Marker from Abcam

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Anti-KDM1/LSD1 antibody - Nuclear Marker from Abcam

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