Anti-ABCA1 antibody [AB.H10] ab18180 from Abcam

Product Specs
ItemAnti-ABCA1 antibody [AB.H10]
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab18180
Antibody TypePrimary
ClonalityMonoclonal
IsotypeIgG1
HostMouse, Nude mouse
Quantity100 UG
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
CloneAB.H10
Conjugate/TagUnconjugated
Concentration1 mg/ml
Research AreaNeuroscience
Gene NameABCA1
ValidationKnockOut Validated
ReactivityHuman, Mouse
TargetABCA1

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1Journal of advanced researchDecember 1, 2023Jiang M, Song Y, Ren MX, He RC, Dong XH, Li XH, Lu ZF, Li S, Wu J, Bei YR, et al.ab2785), anti-alpha smooth muscle actin (αSMA) antibody (Abcam, ab32575), anti-anti-ABCA1 antibody (Abcam, ab18180), anti-ABCG1 antibody (Abcam, ab217023), anti-ICAM1 antibody (Abcam, ab119871), anti-VCAM1 antibody

(2) An iPSC-derived astrocyte model of fragile X syndrome exhibits dysregulated cholesterol homeostasisCommunications BiologyJuly 29, 2023Karo Talvio, Victoria A. Wagner, Rimante Minkeviciene, Jay S. Kirkwood, Anna O. Kulinich, Juzoh Umemori, Anil Bhatia, Manhoi Hur, Reijo Käkelä, Iryna M. Ethell, et al.mounted with Vectashield mounting media. Primary antibodies were mouse anti-ABCA1 antibody (1:500, Abcam, ab18180) and rabbit anti-GFAP (1:500, Cell Signaling Technology, 12389 T). Secondary antibodies were FITC-conjugated

(3) TRIM64 promotes ox-LDL-induced foam cell formation, pyroptosis, and inflammation in THP-1-derived macrophages by activating a feedback loop with NF-κB via IκBα ubiquitinationCell biology and toxicologyJune 1, 2023Zhu C, Chen W, Cui H, Huang Z, Ding R, Li N, Wang Q, Wu F, Zhao Y, Cong X.TRIM64 (Novus Biologicals, LLC, Centennial, CO, USA; NBP2-83,713), CD36 (Abcam; ab133625), ABCA1 (Abcam; ab18180), NF-κB (Abcam; ab16502), NLRP3 (Abcam; ab232401), ASC (Abcam; ab155970), caspase-1 (Abcam; ab207802),

(4) Sulforaphane Inhibits Foam Cell Formation and Atherosclerosis via Mechanisms Involving the Modulation of Macrophage Cholesterol Transport and the Related PhenotypeNutrientsApril 28, 2023Liu S, Zhang Y, Zheng X, Wang Z, Wang P, Zhang M, Shen M, Bao Y, Li D.Santa Cruz Biotechnology), anti-Nrf2 (12721; 1:1000; Cell Signaling Technology, Danvers, MA, USA), anti-ABCA1 (ab18180; 1:500; Abcam) and anti-ABCG1 (ab52617; 1:1000; Abcam), followed by incubation with the corresponding

(5) TNFα Activates the Liver X Receptor Signaling Pathway and Promotes Cholesterol Efflux from Human Brain Pericytes Independently of ABCA1International journal of molecular sciencesMarch 22, 2023Dib S, Loiola RA, Sevin E, Saint-Pol J, Shimizu F, Kanda T, Pahnke J, Gosselet F.Target,kDa,Reference,Supplier,Conditions,Dilution ABCA1,254,ab18180,Abcam,non-heated/reduced,1:1000 ABCB1 (P-gp),180,GTX23364,Genetex,heated/reduced,1:500 ABCG2 (BCRP),72,Ab207732,Abcam,non-heated/reduced,1:1000 ABCG4,100,PA5-34855,Invitrogen,heated/reduced,1:1000 SR-BI,80,ab52629,Abcam,heated/reduced,1:1000 HMGCR,100,Mab90619,Sigma,non-heated/reduced,1:1000 LRP1,80,sc57351,Santa Cruz

(6) Genetic dissection of the impact of lncRNA AI662270 during the development of atherosclerosisJournal of Translational MedicineFebruary 8, 2023Yang Hong, Yue Zhang, Hui Chen, Xueqing Tang, Hongrui Zhao, Ziyu Meng, Xueling Jia, Wenfeng Liu, Xiaohan Li, Lin Wang, et al.Immunofluorescence staining was performed according to the manufacturer’s instructions. Antibody to Abca1 (ab18180, 1:150; Abcam, Cambridge, MA, USA) was used. Cell culture Peritoneal macrophages from adult male WT

(7) MiR-145 is upregulated in the retarded preimplantation embryos and modulates cholesterol levels in mice preimplantation embryos through targeting Abca1Reproductive Biology and EndocrinologyDecember 12, 2022Ou Jian, Ni MengXia, Xing Shiyu, Meng QingXia, Zou QinYan, Ding Jie, Wang Wei, Wan Jiaojiao, Li Hong, Huang YiningBiotech, Herts, UK). Blots were then proved overnight with monoclonal mouse anti-ABCA1 (Abcam, MA, USA; ab18180) or mouse anti-GAPDH (Abcam, MA, USA; ab8245) at 4 °C. Appropriate HRP-conjugated anti-mouse IgG secondary

(8) Role of miR-199a-5p in the post-transcriptional regulation of ABCA1 in response to hypoxia in peritoneal macrophagesFront Cardiovasc MedNovember 3, 2022Juan Francisco Aranda, Ana PÃ©rez-GarcÃa, Marta Torrecilla-Parra, Mario FernÃ¡ndez-de Frutos, Yolanda MartÃn-MartÃn, Pedro A. Mateos-GÃ³mez, Virginia Pardo-MarquÃ©s, Rebeca Busto, Cristina M. RamÃrezwere analyzed by SDS-PAGE and immunoblotting. Primary antibodies used include the following: ABCA1 (#ab18180, Abcam), ABCG1 (#NB400-132), Cav-1 (#SC-894) and HSP90 (#610419, BD Bioscience). Secondary antibodies

(9) Dysregulation of Placental Lipid Hydrolysis by High-Fat/High-Cholesterol Feeding and Gestational Diabetes Mellitus in MiceInternational Journal of Molecular SciencesOctober 14, 2022Katharina B. Kuentzel, Ivan BradiÄ‡, Zala N. MihaliÄ, Melanie Korbelius, Silvia Rainer, Anita Pirchheim, Julia Kargl, Dagmar Kratkyby SDS-PAGE and transferred to a PVDF membrane. The following anti-mouse antibody was used: ABCA1 (ab18180, 1:1000, Abcam, Cambridge, UK), and PonceauS as a loading control. 4.9. Statistical Analysis Statistical

(10) Targeted Suppression of miRNA-33 Using pHLIP Improves Atherosclerosis RegressionCirculation researchJune 24, 2022Zhang X, Rotllan N, Canfrán-Duque A, Sun J, Toczek J, Moshnikova A, Malik S, Price NL, Araldi E, Zhong W, et al.analyzed by SDS-PAGE and immunoblotting. Primary antibodies used include the following: anti-ABCA1 (Abcam, ab18180), anti-ABCG1 (Novus Biologicals, NB400–132) and anti-HSP90 (BD Biosciences, #610419). Secondary antibodies

(11) Mid-pregnancy poly(I:C) viral mimic disrupts placental ABC transporter expression and leads to long-term offspring motor and cognitive dysfunctionScientific ReportsJune 17, 2022V. R. S. Monteiro, C. B. V. Andrade, H. R. Gomes, M. W. Reginatto, G. E. Império, K. N. Fontes, D. A. Spiess, W. S. Rangel-Junior, V. M. O. Nascimento, C. O. S. Lima, et al.(1:500—[Sc55510]; Santa Cruz Biotechnology, USA), anti-Bcrp (1:100—[MAB4146]; Merck Millipore), anti-Abca1 (1:200—[ab18180]; Abcam Plc, UK) or anti-Abcg1 (1:100—[PA5-13462]; Thermo Fisher, USA). The next day, the slides were

(12) Novel role for caspase 1 inhibitor VX765 in suppressing NLRP3 inflammasome assembly and atherosclerosis via promoting mitophagy and efferocytosisCell death & diseaseMay 31, 2022Jin Y, Liu Y, Xu L, Xu J, Xiong Y, Peng Y, Ding K, Zheng S, Yang N, Zhang Z, et al.anti-myc (Santa Cruz Biotechnology, sc-40), anti-gasdermin D (Abcam, ab209845), anti-ABCA1 (Abcam, ab18180), anti-CD36 (Novus Biologicals, NB400-144), anti-Parkin (Cell Signaling Technology, #4211), anti-phosphorylated

(13) Suppression of the ABCA1 Cholesterol Transporter Impairs the Growth and Migration of Epithelial Ovarian CancerCancers (Basel)April 8, 2022Jixuan Gao et al.were loaded onto SDS-PAGE gels. The antibodies used were against ABCA1 (mouse monoclonal (AB.H10) #Ab18180; Abcam, Cambridge, UK; 1:750 to 1:1000), ß-actin (rabbit polyclonal #A2066; Sigma-Aldrich, St. Louis,

(14) Modified Yuejuwan Inhibited Cholesterol Accumulation and Inflammation in THP-1 Macrophage-Derived Foam Cells by Inhibiting the Activity of the TRIM37/TRAF2/NF-ÎºB PathwayEvidence-Based Complementary and Alternative MedicineMarch 10, 2022Mingtai Gui, Lei Yao, Bo Lu, Jianhua Li, Jing Wang, Xunjie Zhou, Deyu FuAbcam, UK, 1 : 1000), TRAF2 (Ab60169, Abcam, UK, 1 : 1500), SR-B1(Ab52629, Abcam, UK, 1 : 1000), ABCA1 (Ab18180, Abcam, UK, 1 : 1000), ABCG1 (Ab52617, Abcam, UK, 1 : 2000), NF-KB (#8242, CST, USA, 1 : 1000), H3 (#4499,

(15) ABCA1 Expression Is Upregulated in an EMT in Breast Cancer Cell Lines via MYC-Mediated De-Repression of Its Proximal Ebox ElementBiomedicinesMarch 2, 2022Prijic S, Chang JT.in non-fat milk for 1 h at room temperature and probed with primary antibodies: anti-ABCA1 (Abcam #ab18180, Cambridge, United Kingdom) at 1:1000, anti-β-actin (Santa Cruz Biotechnology #sc-47778, Dallas, TX,

(16) Abundance of Nef and p-Tau217 in Brains of Individuals Diagnosed with HIV-Associated Neurocognitive Disorders Correlate with Disease SeveranceMolecular neurobiologyFebruary 1, 2022Pushkarsky T, Ward A, Ivanov A, Lin X, Sviridov D, Nekhai S, Bukrinsky MI.217 (Thermo Fisher, cat. #44,744); ABCA1—using mouse monoclonal anti-ABCA1 antibody H10 (Abcam, cat. #ab18180); and flotillin 1—using goat anti-flotillin 1 polyclonal antibody (Novus Biologicals, cat. #NB1001043). Mass Spectrometry Analysis

(17) MicroRNA-320b Modulates Cholesterol Efflux and AtherosclerosisJournal of Atherosclerosis and ThrombosisFebruary 1, 2022Xiaomei Lu, Bin Yang, Huijun Yang, Laiyuan Wang, Hongfan Li, Shufeng Chen, Xiangfeng Lu, Dongfeng GuAntibodies,Catalogue,Source,Working concentration mouse anti-ABCA1,ab18180,Abcam,1:500 rabbit anti-ABCG1,ab52617,Abcam,1:1000 rabbit anti-EEPD1,ab220501,Abcam,1:1000 rabbit

(18) Crocin ameliorates atherosclerosis by promoting the reverse cholesterol transport and inhibiting the foam cell formation via regulating PPARγ/LXR-αCell CycleJanuary 17, 2022Feng Zhang, Peng Liu, Zhaopeng He, like Zhang, Xinqi He, Feng Liu, Jinsheng Qiab176323, Abcam, UK), PCSK9 (1:2000, ab31762, Abcam, UK), CD36 (1:1000, ab133625, Abcam, UK), ABCA1 (1:500, ab18180, Abcam, UK), phosphorylated (p)-AKT (1:1000, ab38449, Abcam, UK), AKT (1:10,000, ab179463, Abcam, UK),

(19) Secretome of endothelial progenitor cells from stroke patients promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakageStem Cell Research & TherapyDecember 1, 2021Rodrigo Azevedo Loiola et al.catenin,Ab6302,Abcam BCRP,Ab3380,Abcam ICAM-1,Ab53013,Abcam VCAM-1,Ab98954,Abcam Tricellulin,Ab253067,Abcam ABCA1,Ab18180,Abcam β-actin,A5541,Sigma Aldrich P-gp,C219,Genetex VE-cadherin,Ab33168,Abcam ZO-1,Ab216880,Abcam Claudin

(20) The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol ExcretionFoodsNovember 18, 2021Jimin Hong, Minji Kim, Bohkyung Kimmonoclonal Anti-Niemann Pick C1-Like 1 antibody,Abcam,ab124801 Mouse monoclonal Anti-ABCA1 antibody,Abcam,ab18180 Rabbit polyclonal Anti-SREBP2 antibody,Abcam,ab30682 Rabbit monoclonal Anti-HMGCR antibody,Abcam,ab174830 Rabbit

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(1)

5/25/2021Great Antibody Gives Good Results Raj KumarCells from human cell line were exposed to a cell growth factor, and cell extract was prepared. The extracted protein was quantified by using BCA reagent, and an equal amount of proteins from control ... Read Review
(1)

5/18/2016Time Course Effect of Chemoattractant on ABCA1 Expression Somasundaram RaghavanATP-binding cassette transporter is a transporter which regulates the cellular cholesterol and phospholipid homeostasis. Here we checked the time course effect of ABCA1 by chemoattractant.

Anti-ABCA1 antibody [AB.H10] from Abcam

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Anti-ABCA1 antibody [AB.H10] from Abcam

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