Cleaved Caspase-3 (Asp175) Antibody 9661S from Cell Signaling Technology

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Molecular Medicine Reports
September 13 2021

Fig 1: Overexpression of FOXO3a promotes cell apoptosis in nephroblastoma cells. (A) Flow cytometric analysis of cell apoptosis in OE-FOXO3a-tranfected cells. (B) Quantification of cell apoptosis from part (A). (C) Western blotting was used to analyze the protein expression levels of apoptosis-related molecules, Bcl-2, Bax, Bim, caspase-3/cleaved caspase-3 in OE-FOXO3a-transfected cells. ***P<0.001 vs. control; ###P<0.001 vs. OE-NC; n=3. FOXO3a, forkhead transcription factor O subfamily 3A; OE, overexpression; NC, negative control; Bim, Bcl-2-like protein 2.
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Cancer Science
May 05 2020

Fig 2: In sunitinib-resistant renal cell carcinoma (RCC) cells, Rapalink-1 had similar effects in vitro and vivo. A, Cell proliferation was determined by XTT assays during treatment with temsirolimus or Rapalink-1 in SU-R-786-o cells from 24 to 96 h. All experiments were performed in quadruplicate. *P < 0.0001. B, Apoptosis assays were carried out using flow cytometry. All experiments were performed in triplicate. *P < 0.001; **P < 0.0001. C, Western blot analysis of apoptotic markers (cleaved PARP). ß-actin was used as a loading control. D, Cell cycle assays were carried out using flow cytometry. The bar charts represent the percentage of mock cells in G0/G1, S or G2/M phases. All experiments were performed in triplicate. *P < 0.05; **P < 0.001. E, Cell migration was determined by wound healing assays. All experiments were performed in triplicate. *P < 0.0001. F, Cell invasion was determined by Matrigel invasion assays. All experiments were performed in triplicate. *P < 0.0001. G, Colony formation by SU-R-786-o cells was remarkably inhibited by Rapalink-1 compared with temsirolimus. All experiments were performed in triplicate. *P < 0.0001. H, SU-R-786-o cells were treated with temsirolimus or RapaLink-1 at 100 nmol/L for 3 h and analyzed for PI3K/AKT/mTOR signaling by western blotting. I, SU-R-786-o tumor-bearing nude mice (n = 5 per group) were given i.p. injections of vehicle (daily), temsirolimus (1.5 mg/kg, daily) or Rapalink-1 (1.5 mg/kg, every 5 d). *P < 0.05. J, Tumors were extracted and immunostained with cleaved caspase-3 and cleaved PARP antibodies. Percentages of cleaved caspase-3 and cleaved PARP positive cells were quantitated by counting 6 random microscopic fields. *P < 0.0001; **P < 0.001; ***P < 0.05
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Genes & development
February 15 2016

Fig 3: Autophagy deficiency generates smaller prostate tumors. (A) Prostate weight as a percentage of body weight at various times after tumor induction. (B) Gross anatomy of urogenital systems of mice with Atg7+/+ and Atg7?/? prostates at various times after tumor induction showing changes in size and cyst formation over time. (C) Representative active caspase-3 and Ki-67 IHC of Atg7+/+ and Atg7?/? anterior mouse prostates at 3 mo after tumor induction. (D) Kaplan-Meier survival curve for mice with Pten?/? Atg7+/+, Pten?/? Atg7+/?, and Pten?/? Atg7?/? prostate tumors.
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Aging
April 08 2020

Fig 4: ANT-DBS reduced seizures frequency and relieved hippocampal neurons apoptosis in the epileptic monkeys. (A–C) Numbers of seizures in the different groups. The numbers of partial and total seizures were reduced by ANT-DBS, compared with the EP and EP-sham-DBS groups. Although there was no significant difference in the number of generalized seizures between EP-sham-DBS and EP-DBS groups (P > 0.05), there was still a trend of reduction. (n=6 in each group) (D) Morphology of hippocampus in monkeys receiving ANT-DBS was not affected and there was no obvious atrophy. Red arrows indicate the location of the hippocampus in the atlas of the rhesus monkey brain. (E) Analysis of NeuN, cleaved-caspase-3 caspase-3, cleaved-caspase-9 and caspase-9 by western blotting. (F–J) There were marked decrease in NeuN level and increase in cleaved-caspase-3 and cleaved-caspase-9 levels in EP and EP-sham-DBS groups. Increased NeuN and reduced cleaved-caspase-3 and cleaved-caspase-9 levels were detected after ANT-DBS. (n=3 in each group) *P< 0.05; **P< 0.01; ***P< 0.001; NS, P> 0.05. Data were presented as mean ± SD. I, control group; II, EP group; III, EP-sham-DBS group; IV, EP-DBS group.
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Investigative Opthalmology & Visual Science
May 04 2022

Fig 5: MLT attenuated apoptosis in LPS-stimulated HMGECs. The HMGECs were treated with MLT for 48 hours followed by LPS for 24 hours. The undifferentiated group was not treated with the differentiated medium. (A) Images of phospho-histone H2A.X foci in HMGECs depicted by immunofluorescence staining. Scale bar: 20 µm. (B) Representative protein bands of phospho-histone H2A.X. (C) Quantitative intensity of phospho-histone H2A.X (n = 4). (D) Images of cleaved caspase 3 in HMGECs obtained by immunofluorescence staining. (E) Fluorescence intensity quantitation of cleaved caspase 3 (n = 3). Scale bar: 20 µm. (F) Representative protein bands of cleaved caspase 3, Bax, and Bcl-2. (G, H) Quantitative intensity of cleaved caspase 3 (G) and Bax/Bcl-2 (H) (n = 4). ***Significantly different from control, ***P < 0.001. ##Significantly different from LPS group, ##P < 0.01, ###P < 0.001.

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Cell Signaling Technology for
Cleaved Caspase-3 (Asp175) Antibody

Cell Signaling Technology's Cleaved Caspase-3 (Asp175) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Simple Western, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot. The Cleaved Caspase-3 (Asp175) Antibody was generated using CASP3 as the antigen and it reacts with Homo Sapiens, Human, Mouse, Rat, and Non-Human Primate.

Description

Cleaved Caspase-3 (Asp175) Antibody detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases. This antibody detects non-specific caspase substrates by western blot. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Nuclear background may be observed in rat and monkey samples

Product Specs
ItemCleaved Caspase-3 (Asp175) Antibody
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9661S
Quantity100 µl
ApplicationsW, W-S, IP, IHC-P, IF-IC, FC-FP
ReactivityH, M, R, Mk
TargetCasp3 (Asp175) cleaved
HostRabbit
NCBI Gene AliasesCPP32, CPP32B, SCA-1
NCBI Full Gene Namecaspase 3
ClonalityPolyclonal
Research AreaApoptosis
Molecular Weight17, 19

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Phone: 978-867-2388
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Website: http://www.cellsignal.com/

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(1) Upregulation of KHDC1L promotes the proliferation and inhibits apoptosis in head and neck squamous cell carcinomaEpigeneticsDecember 31, 2023Qi Zhang, Shuimei Luo, Yang Luo, Yiqiang Huang, Ziming Wang, Xianhe Xie(#13038, 1:1000), AKT (#4691, 1:3000), Bcl-2 (#15071, 1:1000), BAX (#5023, 1:1000) and cleaved Caspase-3 (#9661, 1:3000) were ordered from Cell Signaling Technology (Cell Signaling Technology, Danvers, MA). Besides,

(2) Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6PCell researchDecember 1, 2023Ai YL, Wang WJ, Liu FJ, Fang W, Chen HZ, Wu LZ, Hong X, Zhu Y, Zhang CX, Liu LY, et al.(Cat# 2535S), anti-AMPKα (Cat# 2603S), anti-phospho-ACC (Cat# 3661S), anti-cleaved caspase-3 (Cat# 9661S), anti-PARP (Cat# 9532) and anti-LKB1 (Cat# 3050S) antibodies were purchased from Cell Signaling Technology.

(3) Human umbilical cord-derived mesenchymal stem cells alleviate oxidative stress-induced islet impairment via the Nrf2/HO-1 axisJournal of molecular cell biologyNovember 27, 2023Liu P, Cao B, Zhou Y, Zhang H, Wang C.#20989), BCL-2 (1:1000, Proteintech, #60718), cleaved Caspase-3 (1:1000, Cell Signaling Technology, #9661S), Nrf2 (1:1000, Abcam, ab137550; 1:1000, Cell Signaling Technology, #33649S), HO-1 (1:1000, Cell Signaling Technology

(4) Adipose mesenchymal stem cell-derived extracellular vesicles reduce glutamate-induced excitotoxicity in the retinaNeural regeneration researchOctober 1, 2023Duan TQ, Gao ZL, Luo AX, Chen D, Tong JB, Huang JF.polyclonal rabbit anti-cleaved caspase 3 (1:1000; Cell Signaling Technology, Danvers, MA, USA, Cat## 9661, RRID: AB_2341188); and monoclonal rabbit anti-β-tubulin (1:10,000; Proteintech, Wuhan, China, Cat#

(5) 3-Bromopyruvate overcomes cetuximab resistance in human colorectal cancer cells by inducing autophagy-dependent ferroptosisCancer gene therapyOctober 1, 2023Mu M, Zhang Q, Zhao C, Li X, Chen Z, Sun X, Yu J.(1:1000, 17168-1-AP) primary antibodies from Proteintech (IL, USA), anti-Cleaved Caspase-3 (1:1000, #9661), anti-phospho-Beclin1 (1:1000, #14717), anti-phosphor-AMPKα (1:1000, #2535), anti-phospho-FOXO3a (1:1000,

(6) Camel nanobody-based B7-H3 CAR-T cells show high efficacy against large solid tumoursNature communicationsSeptember 22, 2023Li D, Wang R, Liang T, Ren H, Park C, Tai CH, Ni W, Zhou J, Mackay S, Edmondson E, et al.coverslipped with Prolong Gold AntiFade Reagent (Invitrogen). Cleaved Caspase-3 (Cell Signaling Technology, #9661) immunohistochemistry was performed to predict cell apoptosis. Images were captured using the Aperio

(7) miR-181b promotes angiogenesis and neurological function recovery after ischemic strokeNeural regeneration researchSeptember 1, 2023Xue LX, Shu LY, Wang HM, Lu KL, Huang LG, Xiang JY, Geng Z, Zhao YW, Chen H.AB_2081719), rabbit anti-cleaved caspase-3 antibody (1:1000, Cell Signaling, Danvers, MA, USA, Cat# 9661, RRID: AB_2341188), rabbit/mouse anti-cleaved caspase-8 antibody (1:1000, Cell Signaling, Cat# 9429,

(8) Hirudin inhibits glioma growth through mTOR-regulated autophagyJournal of cellular and molecular medicineSeptember 1, 2023Ma Y, Wu S, Zhao F, Li H, Li Q, Zhang J, Li H, Yuan Z.following antibodies were purchased from Cell Signalling Technology: Caspase‐3 (9662), cleaved‐Caspase3 (9661), p‐ULK1 (14202), ULK1 (8054), p‐P70S6K (9234), P70S6K (2708), p‐4EBP1 (2855). LC3 (14600‐1‐AP), P62/SQSTM1

(9) Extracellular Vesicles Play a Central Role in Cerebral Venous Disease-Associated Brain AtrophyAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2023Wang JY, Li JY, Luo D, Huang MY, Ao DH, Liu XN, Wang X, Ge W, Zhu YC.PSD95 (1:200, 124 011, Synaptic Systems), NeuN (1:200, ab177487, Abcam), Cleaved Caspase 3 (1:200, 9661, Cell Signaling Technology) and Alexa Fluor 488‐Phalloidin (1:200, A12379, Thermo Fisher Scientific).

(10) Antioxidant mitoquinone suppresses benign prostatic hyperplasia by regulating the AR-NLRP3 pathwayRedox biologySeptember 1, 2023Jin BR, Lim CY, Kim HJ, Lee M, An HJ.antibodies against prostate-specific antigen (PSA; Cat. No. PB9259). Antibodies against caspase-3 (Cat. No. #9661) were purchased from Cell Signaling Technology. Novus Biologicals (CO, USA) provided antibodies against

(11) CRTAC1 enhances the chemosensitivity of non-small cell lung cancer to cisplatin by eliciting RyR-mediated calcium release and inhibiting Akt1 expressionCell death & diseaseAugust 26, 2023Jin Z, Zhao L, Chang Y, Jin R, Hu F, Wu S, Xue Z, Ma Y, Chen C, Zheng M, et al.ab102548, RRID: AB_10710442) was purchased from Abcam (Cambridge, UK). Antibodies against cleaved caspase3 (9661S), Akt (pan) (4685S), Akt1 (75692S), p-Akt (ser473) (4060S), and STUB1 (2080S) were obtained from Cell Signaling Technology

(12) Targeting ANXA7/LAMP5-mTOR axis attenuates spinal cord injury by inhibiting neuronal apoptosis via enhancing autophagy in miceCell death discoveryAugust 24, 2023Li N, Chen L, Zhao X, Gu C, Chang Y, Feng S.Cat# 67778-1-Ig), GAPDH (CST, Cat# 5174 S), beta-actin (CST, Cat# 4970 S), Cleaved-caspase3 (CST, Cat# 9661 S). After overnight incubation, the membrane was washed with TBST for three times (5 min per time) and

(13) Magneto-mechanical stimulation modulates osteocyte fate via the ECM-integrin-CSK axis and wnt pathwayiScienceAugust 18, 2023Zhang B, Li X, Zhou X, Lou C, Wang S, Lv H, Zhang G, Fang Y, Yin D, Shang P.Cleaved Caspase-3 (Asp175) Antibody (1:1000 dilution in 1× TBST, Cell Signaling Technologies, Cat# 9661), rabbit anti-LAMB 1 antibody (1:1000 dilution in 1× TBST, Proteintech, Cat#23498-1-AP), rabbit anti-collagen

(14) SQLE promotes pancreatic cancer growth by attenuating ER stress and activating lipid rafts-regulated Src/PI3K/Akt signaling pathwayCell death & diseaseAugust 4, 2023Xu R, Song J, Ruze R, Chen Y, Yin X, Wang C, Zhao Y.(#12544-1-AP, Proteintech; 1:200), anti-Ki67 (#27309-1-AP, Proteintech; 1:2500), and anti-cleaved caspase 3 (#9661, Cell Signaling Technology; 1:200). Lentiviral infection Genechem (Shanghai, China) supplied lentiviruses

(15) A Mixture of Fermented Schizandrae Fructus Pomace and Hoveniae Semen cum Fructus Extracts Synergistically Protects against Oxidative Stress-Mediated Liver InjuryAntioxidants (Basel)August 3, 2023Jang-Soo Kim, Kyung-Hwan Jegal, Hye-Rim Park, Beom-Rak Choi, Jae-Kwang Kim, Sae-Kwang Kuperoxidase activity. After incubation with anti-cleaved caspase-3 (1:400, Cell Signaling Technology, #9661), anti-cleaved PARP (1:100, Santa Cruz Biotechnology, #sc-23461), anti-4-HNE (1:100, Abcam, #ab46545),

(16) Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolismJCI insightAugust 3, 2023Abdon B, Liang Y, da Luz Scheffer D, Torres M, Shrestha N, Reinert RB, Lu Y, Pederson B, Bugarin-Lapuz A, Kersten S, et al.anti–caspase-3 (Cell Signaling Technology, 9662; 1:1000), anti–cleaved caspase-3 (Cell Signaling Technology, 9661; 1:1000), anti-IRE1α (Cell Signaling Technology, 3294; 1:1000), anti-PERK (Cell Signaling Technology,

(17) Infection of porcine enteroids and 2D differentiated intestinal epithelial cells with rotavirus A to study cell tropism and polarized immune responseEmerging Microbes & InfectionsAugust 2, 2023Miaomiao Yan, Ang Su, Suvarin Pavasutthipaisit, Rebecca Spriewald, Guntram A. Graßl, Andreas Beineke, Doris Hoeltig, Georg Herrler, Paul BecherChromogranin A antibody (C-12),Sc-393941,Santa Cruz Rabbit anti Cleaved Caspase-3 (Asp175) antibody,9661,Cell Signalling Goat anti rotavirus polyclonal antibody,PA1-7241,Life Cy™3 Affini Pure Goat Anti-Mouse IgG (H + L

(18) Monoacylglycerol lipase reprograms hepatocytes and macrophages to promote liver regenerationJHEP ReportsAugust 1, 2023Manon Allaire, Rola Al Sayegh, Morgane Mabire, Adel Hammoutene, Matthieu Siebert, Charles Caër, Mathilde Cadoux, JingHong Wan, Aida Habib, Maude Le Gall, et al.(1:100, Dako M7240), and a cleaved caspase-3 (Asp175) (1:50, Cell Signaling Technology, Danvers, MA, USA, 9661) antibodies. Anti-rat (Vector Laboratories BA-9401) or anti-rabbit antibody (Bio-Rad STAR131B) was used

(19) MNX1 facilitates the malignant progress of lung adenocarcinoma through transcriptionally upregulating CCDC34Oncology lettersAugust 1, 2023Wu J, Yue C, Xu W, Li H, Zhu J, Li L.Abcam), Bax (dilution 1:1,000; cat. no. ab32503; Abcam), cleaved-caspase3 (dilution 1:1,000; cat. no. 9661; Cell Signaling Technology, Inc.), cleaved-caspase9 (dilution 1:1,000; cat. no. 7237; Cell Signaling Technology

(20) Lipid emulsion inhibits the cardiac toxicity caused by chloroquine via inhibition of reactive oxygen species productionKorean journal of anesthesiologyAugust 1, 2023Lee SH, Ok SH, Ahn SH, Sim G, Kim HJ, Kim M, Yoon S, Sohn JT.caspase-8 antibody was purchased from Novus Biologicals (#NB100-56116, USA). Anti-cleaved caspase-3 (#9661) and anti-Bax (#2772) antibodies were obtained from Cell Signaling Technology. All drugs were dissolved

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2/9/2024Cleaved Caspase-3 Ab From Cell Signaling Was Used as a Marker of Cell Apoptosis After DMAG-17 Chemotherapy Treatment. Ksenia NovototskayaActivation of endogenous retrotransposons frequently occurs in cancer cells and contributes to tumor genomic instability. To test whether inhibition of retrotranspositions has an anticancer effect, we... Read Review
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10/9/2023Decent Cleaved Caspase-3 Antibody From Cell Signaling for Western Blot Tobias BeiglI used this antibody several times for the detection of apoptosis induction in whole-cell lysates of prostate carcinoma cells. The detection did not work right away and needed to be optimized. However... Read Review
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7/22/2022Cleaved Caspase 3 (Asp175) Antibody for Western Blot Ines RThis antibody was used to detect cleaved caspase-3 levels in western blot analysis. Protein loading: 25 ug/mL.
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2/1/2022Reliable Detection of Cleaved Caspase-3 Xie ZhangThis antibody is used to detect the activation of Cleaved Caspase-3 in cells.
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8/13/2020Rabbit anti-Caspase-3 cleaved (Asp175) Antibody for Immunofluorescence José VieiraThis antibody works really well to detect cells in apoptosis during development.
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12/26/2019Good Caspase-3 Antibody from CST Purnima GuptaExcellent antibody
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12/18/2019Good Antibody to Assess Cell Death Via Caspase-3 CleavageDr. Min Hee OhGreat antibody to assess cell death via western blotting.
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2/19/2019Cleaved Caspase-3 Antibody for ImmunofluorescenceDr. Efstathios StamatiadesReliable & reproducible staining of apoptotic cells
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4/17/2018Time Course Effect Of Chemoattractant On Cleaved Caspase-3 Expression Somasundaram RaghavanCaspase-3 is a member of cysteine aspartic acid protease family. CASP3 is a key regulator for apoptosis, and it is responsible for proteolytic cleavage of many proteins such as poly (ADP-ribose) ... Read Review
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2/1/2017Cleaved Caspase 3 Antibody Tara VanderweydeWe have used this antibody successfully on Western blots to look at active caspase 3, this is the one we find to work best. When we were looking for a cleaved caspase 3 marker for staining we gave ... Read Review

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Cleaved Caspase-3 (Asp175) Antibody from Cell Signaling Technology

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Cleaved Caspase-3 (Asp175) Antibody from Cell Signaling Technology

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