Anti-rabbit IgG, HRP-linked Antibody 7074S from Cell Signaling Technology

Product Specs
ItemAnti-rabbit IgG, HRP-linked Antibody
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number7074S
Quantity1 ml
HostGoat
Conjugate/TagHRP
Research AreaRelated Products
ApplicationsW
TargetRabbit IgG

Add to Compare List

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) SARS-CoV-2 Nsp1 mediated mRNA degradation requires mRNA interaction with the ribosomeRNA BiologyDecember 31, 2023Soraya I. Shehata, Roy Parker(Santa Cruz, sc -47724 HRP), anti-rabbit IgG HRP-linked secondary antibody (Cell Signaling Technology, 7074S) were used at 1:1000. Protein purification Recombinant wtNsp1 and mutants were expressed and purified

(2) Upregulation of KHDC1L promotes the proliferation and inhibits apoptosis in head and neck squamous cell carcinomaEpigeneticsDecember 31, 2023Qi Zhang, Shuimei Luo, Yang Luo, Yiqiang Huang, Ziming Wang, Xianhe XieIgG(H + L) (#RS0001,1:5000) from ImmunoWay (Jiangsu, China) and anti-rabbit IgG HRP-linked antibody (#7074, 1:5000) from Cell Signaling Technology. Cell morphology, CCK8 assays, cell counting assays, and colony

(3) Design, synthesis and biological evaluation of 2-((4-sulfamoylphenyl)amino)-pyrrolo[2,3-d]pyrimidine derivatives as CDK inhibitorsJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2023Bo Yang, Yanni Quan, Wuli Zhao, Yingjie Ji, Xiaotang Yang, Jianrui Li, Yi Li, Xiujun Liu, Ying Wang, Yanping Li1:1,000), c-Myc (Abcam #YB69; 1:1000). Secondary antibodies were from Cell Signalling Technology (#7076 or #7074). ECL detection was performed. Cell cycle and apoptosis analysis MIA PaCa-2 cells were incubated with

(4) Crosstalk between m6A mRNAs and m6A circRNAs and the time-specific biogenesis of m6A circRNAs after OGD/R in primary neuronsEpigeneticsDecember 31, 2023Chi Zhang, Huan Jian, Shenghui Shang, Lu Lu, Yongfu Lou, Yi Kang, Hong Bai, Zheng Fu, Yigang Lv, Xiaohong Kong, et al.secondary anti-rabbit IgG antibody (1: 2000, Cell Signalling Technology, Danvers, MA, USA. Cat. No. 7074S) for 1 h at room temperature. After 3 times washes with TBST, the membrane was visualized by using Immobilon Western Chemiluminescent HRP Substrate

(5) The crosstalk between microbial sensors ELMO1 and NOD2 shape intestinal immune responsesVirulenceDecember 31, 2023Aditi Sharma, Sajan Chandrangadhan Achi, Stella-Rita Ibeawuchi, Mahitha Shree Anandachar, Hobie Gementera, Uddeep Chaudhury, Fatima Usmani, Kevin Vega, Ibrahim M Sayed, Soumita DasHRP-linked Antibody (Cat # 7076S, Cell Signaling Tech) or anti-rabbit IgG HRP-linked antibody (Cat #7074S, Cell Signalling Tech). Protein bands were detected using ECL (Amersham Biosciences). Western blot was

(6) Repurposing of rabeprazole as an anti-Trypanosoma cruzi drug that targets cellular triosephosphate isomeraseJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2023Itzhel García-Torres, Ignacio De la Mora-De la Mora, Gabriel López-Velázquez, Nallely Cabrera, Luis Antonio Flores-López, Ingeborg Becker, Juliana Herrera-López, Roberto Hernández, Ruy Pérez-Montfort, Sergio Enríquez-Flores(diluted 1:3000) conjugated with horseradish peroxidase (HRP) (Anti-rabbit IgG, HRP-linked Antibody #7074, Cell Signalling Technology, Danvers, Massachusetts, USA) was used to reveal the immunoblot bands by

(7) Glutathione metabolism rewiring protects renal tubule cells against cisplatin-induced apoptosis and ferroptosisRedox ReportDecember 31, 2023Xing-Qiang Dong, Li-Kai Chu, Xu Cao, Qian-Wei Xiong, Yi-Ming Mao, Ching-Hsien Chen, Yun-Li Bi, Jun Liu, Xiang-Ming Yananti-mouse HRP-linked antibody (7076S, Cell Signaling, 1:5000 dilution), anti-rabbit HRP-linked antibody (7074S, Cell Signaling, 1:5000 dilution). Mouse model of cisplatin-induced AKI and drug administration Six

(8) Targeting endoplasmic reticulum associated degradation pathway combined with radiotherapy enhances the immunogenicity of esophageal cancer cellsCancer Biology & TherapyDecember 31, 2023Hui Luo, Yanan Sun, Liuxiang Wang, Hui Liu, Ran Zhao, Mengqiu Song, Hong Ge#4970, Na,K-ATPase Antibody #3010, CST) and secondary antibody (Anti-rabbit IgG, HRP-linked Antibody #7074, CST). Finally, the membranes were imaged with an enhanced chemiluminescence detection reagent (Meilunbio,

(9) Augmented interferon regulatory factor 7 axis in whole tumor cell vaccines prevents tumor recurrence by inducing interferon gamma-secreting B cellsOncoImmunologyDecember 31, 2023Nabeel Kajihara, Yoshino Tanaka, Riko Takeuchi, Takuto Kobayashi, Masafumi Tanji, Tsukasa Ataka, Shiho Nakano, Taisho Yamada, Akinori Takaoka, Yoshinori Hasegawa, et al.by incubation in 1:3000 dilution of horseradish peroxidase-conjugated anti-rabbit IgG antibodies (#7074S, Cell Signaling Technology) at room temperature for 30 min with gentle shaking. Proteins were visualized

(10) Early-life stress triggers long-lasting organismal resilience and longevity via tetraspaninbioRxiv : the preprint server for biologyDecember 17, 2023Jiang WI, De Belly H, Wang B, Wong A, Kim M, Oh F, DeGeorge J, Huang X, Guang S, Weiner OD, et al.washes of 5 min each with tris-buffered saline with 0.1% Tween 20, anti-rabbit IgG, HRP-linked Antibody (7074S, Cell Signaling Technology) was added at a dilution of 1:5000. For native-PAGE samples, stage-synchronized

(11) PRR14 organizes H3K9me3-modified heterochromatin at the nuclear laminaNucleus (Austin, Tex.)December 1, 2023Kiseleva AA, Cheng YC, Smith CL, Katz RA, Poleshko A.A31571, IF 1:1000); and HRP-linked anti-Rabbit IgG and HRP-linked anti-Mouse IgG (Cell Signaling, cat# 7074 and 7076; WB 1:5,000). IF – immunofluorescence, WB – Western Blot, IP – immunoprecipitation. Statistical

(12) Glucocorticoid receptors involved in ginsenoside compound K ameliorate adjuvant arthritis by inhibiting the glycolysis of fibroblast-like synoviocytes via the NF-κB/HIF-1α pathwayPharm BiolDecember 1, 2023Wang Y, Bao X, Xian H, Wei F, Song Y, Zhao S, Zhang Y, Wang Y, Wang Y.antibodies (anti-mouse IgG, HRP-linked antibody, CST, 7076; and anti-rabbit IgG, HRP-linked antibody, CST, 7074) at room temperature for 2 h on the second day. After washing with PBST, the blots were analyzed using

(13) ANGPTL2-mediated epigenetic repression of MHC-I in tumor cells accelerates tumor immune evasionMolecular oncologyDecember 1, 2023Kadomatsu T, Hara C, Kurahashi R, Horiguchi H, Morinaga J, Miyata K, Kurano S, Kanemaru H, Fukushima S, Araki K, et al.were incubated 1 h with 1 : 3000 diluted horseradish peroxidase (HRP)‐conjugated anti‐rabbit IgG (#7074; Cell Signaling Technology) or HRP‐conjugated sheep anti‐mouse IgG (NA9310; GE Healthcare Life Science,

(14) Hepatoprotective effect of protocatechuic acid against type 2 diabetes-induced liver injuryPharm BiolDecember 1, 2023Xu K, Lu G, Feng Q, Chen S, Wang Y.#8480, CST), interleukin (IL)-1β (26048-1-AP, Proteintech), β-actin (#4970, CST), goat-anti-rabbit IgG (#7074, CST) and goat-anti mouse IgG (#7076, CST). Quantitative real-time PCR (qRT-PCR) assays Total RNA

(15) Association with cationized gelatin nanospheres enhances cell internalization of mitochondria efficiencyRegenerative TherapyDecember 1, 2023Wenxuan Yang, Satoshi Abe, Yasuhiko Tabataanti-β-Actin,Cell Signaling Technology,4970S,1:1400 HRP-linked goat anti-rabbit IgG,Cell Signaling Technology,7074S,1:2000 HRP-linked horse anti-mouse IgG,Cell Signaling Technology,7076S,1:2000 Antibodies used for western blotting

(16) Defective EMC1 drives abnormal retinal angiogenesis via Wnt/β-catenin signaling and may be associated with the pathogenesis of familial exudative vitreoretinopathyGenes & DiseasesNovember 1, 2023Shujin Li, Mu Yang, Rulian Zhao, Li Peng, Wenjing Liu, Xiaoyan Jiang, Yunqi He, Erkuan Dai, Lin Zhang, Yeming Yang, et al.antibody (7076, Cell Signaling Technologies, USA), and HRP-conjugated goat anti-rabbit secondary antibody (7074, Cell Signaling Technologies, USA). Transcriptome sequencing and bioinformatic analysis A high-quality

(17) Cynaropicrin disrupts tubulin and c-Myc-related signaling and induces parthanatos-type cell death in multiple myelomaActa pharmacologica SinicaNovember 1, 2023Boulos JC, Omer EA, Rigano D, Formisano C, Chatterjee M, Leich E, Klauck SM, Shan LT, Efferth T.either anti-rabbit IgG secondary antibody coupled to horseradish peroxidase (HRP-linked; 1:1000) (#7074S, Cell Signaling Technology, Frankfurt a. M., Germany) or anti-mouse IgG secondary antibody coupled to

(18) SEPTIN 7 INTERACTS WITH NUMB TO PRESERVE SARCOMERE STRUCTURAL ORGANIZATION AND MUSCLE CONTRACTILE FUNCTIONbioRxiv : the preprint server for biologyOctober 16, 2023De Gasperi R, Csernoch L, Dienes B, Gonczi M, Chakrabarty JK, Goeta S, Aslan A, Toro CA, Karasik D, Brown LM, et al.in TBST and incubated with HRP -conjugated anti-rabbit IgG at 1:2000 dilution in blocking solution (#7074, Cell Signaling Technology). Bands were revealed with ECL Prime reagent (Cytiva, Lifesciences, Piscataway,

(19) Comprehensive isotopomer analysis of glutamate and aspartate in small tissue samplesCell metabolismOctober 3, 2023Cai F, Bezwada D, Cai L, Mahar R, Wu Z, Chang MC, Pachnis P, Yang C, Kelekar S, Gu W, et al.were detected with a horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology, 7074S, 1:2000 dilution) for 1 hour followed by exposure to ECL reagents (Fisher Scientific, PI32106). Standard Preparation

(20) The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situJournal of Cell BiologyOctober 2, 2023Lukas A. Fuentes, Zach Marin, Jonathan Tyson, David Baddeley, Joerg Bewersdorffollowed by incubation with secondary antibodies diluted in block buffer (goat anti-rabbit-HRP at 1:2,000: 7074S; Cell Signaling Technology; horse anti-mouse-HRP at 1:2,000: 7076S; Cell Signaling Technology) for 1

Write a Review

(1)

8/11/2022Great Secondary Antibody Aditi KothariI used this secondary antibody with a lot of my anti-rabbit primary antibodies. It works great and can help me detect proteins that are usually difficult to detect using fluorescent means.
(1)

5/20/2022Good Secondary Antibody for Western Blot Daniel HamiltonI used this antibody several times and I always got great results. I diluted 1:10000 with 5% BSA in TBST and I incubated my membranes from 1 to 4 hours depending on the primary antibody at RT.
(1)

12/8/2020Nice Antibody for Western Blot Sachin SamuchiwalI used this as the secondary antibody to probe primary antibody (with rabbit as host) in Western blotting. Here, I determined the levels of endogenous Beta actin in THP1 cells treated with different ... Read Review
(1)

6/17/2020Anti-Rabbit IgG HRP Antibody Is Very Suitable for Western Blotting Ab GThere is not much non-specificity with this secondary antibody. It is very comparable with other available rabbit secondary antibodies in the market.
(1)

1/23/2020Consistent Results for Anti-Rabbit Secondary on Western Blot Matt SunThis is a required anti-rabbit antibody for secondary. It works so well for western blots and there is always consistent results.
(1)

11/5/2018Very Strong Secondary Antibody for Western Blot Meng SunOur lab is interested in T cells and tumor cells in relation to metabolism. We use this secondary antibody for western blot after incubation with the primary overnight.
(1)

10/25/2018Detect Protein of Interest Using Anti-Rabbit Secondary Antibody Sadaf AtarodThe aim of this experiment was to detect Caspase-3 protein. The primary antibody was rabbit-derived, hence this anti-rabbit secondary antibody was recommended by colleagues.
(1)

10/3/2017Anti-Rabbit HRP-linked Secondary Antibody Tyler MattoxThis HRP-linked secondary antibody is used for the detection of proteins that are probed with a rabbit antibody. I use this secondary antibody often for the detection of GAPDH in MIA PaCa-2 human ... Read Review
(1)

3/9/2016Good Anti-Rabbit HRP-Linked Secondary Antibody Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.

BMC Genomics

Frontiers in Genetics

Anti-rabbit IgG, HRP-linked Antibody from Cell Signaling Technology

Cell Signaling Technology

Citations (11070)

Reviews

Anti-rabbit IgG, HRP-linked Antibody from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map