beta3-Tubulin (D65A4) XP ® Rabbit mAb 5666S from Cell Signaling Technology

Product Specs
Itembeta3-Tubulin (D65A4) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number5666S
Quantity100 µl
ApplicationsW, IP, IHC-P
ReactivityH, M, R
Targettubulin beta-3
HostRabbit
NCBI Gene AliasesCDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4
ClonalityMonoclonal
NCBI Full Gene Nametubulin beta 3 class III
Research AreaNeuroscience
IsotypeIgG
CloneD65A4
Molecular Weight55

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Immune-regulating strategy against rheumatoid arthritis by inducing tolerogenic dendritic cells with modified zinc peroxide nanoparticlesJournal of NanobiotechnologyDecember 1, 2022Han Qiao, Jingtian Mei, Kai Yuan, Kai Zhang, Feng Zhou, Tingting Tang, Jie ZhaoThr172 (50,081), IKKα (61,294), Iκbα (4814), p65 (8242), Akt (9272), AMPK (5832), GAPDH (5174), tubulin (5666), and IgG (2729) were purchased from Cell Signaling Technology. TRIzol reagents were purchased from

(2) A “multi-omics” analysis of blood–brain barrier and synaptic dysfunction in APOE4 miceJournal of Experimental MedicineNovember 7, 2022Giuseppe Barisano, Kassandra Kisler, Brent Wilkinson, Angeliki Maria Nikolakopoulou, Abhay P. Sagare, Yaoming Wang, William Gilliam, Mikko T. Huuskonen, Shu-Ting Hung, Justin K. Ichida, et al.(CBL242, 1:500 dilution; Chemicon); rabbit anti-human β3 tubulin (TuJ-1), cross-reacts with mouse TuJ-1 (5666, 1:1,000 dilution; Cell Signaling); rabbit anti-mouse Cldn-5 (34–1,600, 1:1,000 dilution; Invitrogen);

(3) A Localized Materials-Based Strategy to Non-Virally Deliver Chondroitinase ABC mRNA Improves Hindlimb Function in a Rat Spinal Cord Injury ModelAdvanced healthcare materialsOctober 1, 2022Khalil AS, Hellenbrand D, Reichl K, Umhoefer J, Filipp M, Choe J, Hanna A, Murphy WL.chondroitin sulfate (Sigma Aldrich C8035 1:100), rabbit anti-ß3-tubulin (Cell Signaling Technologies 5666S 1:300). Secondary antibodies and dilutions: goat anti-chicken 488 (A-21449), goat anti-mouse 590 (A-11004),

(4) Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesisSTAR ProtocolsJune 17, 2022Sho Tanosaki, Tomohiko Akiyama, Sayaka Kanaami, Jun Fujita, Minoru S.H. Ko, Keiichi Fukuda, Shugo Tohyamaantibodies against OCT4 (Santa Cruz Biotechnology, sc-5279, 1:1000) and βIII-tubulin (Cell Signaling, 5666, 1:1000) with ImmunoBlock.e.Apply the diluted antibodies to the cells and incubate for 3 h at room temperature.f.Remove

(5) Ginkgolic acid improves bleomycin-induced pulmonary fibrosis by inhibiting SMAD4 SUMOylationOxidative Medicine and Cellular LongevityJune 6, 2022Lan Yu et al.Antibody,Company,Code,Application,Dilution SUMO1,Abcam,ab11672,WB,1 : 1000 UBC9,Abcam,ab75854,WB,1 : 2000 SENP1,Abcam,ab108981,WB,1 : 4000 β3-tubulin,CST,#5666,WB,1 : 2000 SMAD2/3,Abcam,ab202445,WB,1 : 1000 SMAD4,Abcam,ab230815,WB,1 : 1000 E-cadherin,BD,610404,WB,1 : 500

(6) Assessment of BMP responses in an in vitro model of acute ethanol toxicityMethodsXJanuary 1, 2022Habeeb N, Najafi S, Perron JC.16;8(7):bio042283. doi: 10.1242/bio.042283" Resource availability;,Recombinant BMP7 (R&D Systems – catalog #5666-BP carrier free)Phospho-Smad1/5/8 (D5B10) Rabbit mAb (Cell Signaling Technology – catalog #13820)Phospho-Akt

(7) A Novel In Vitro Assay Using Human iPSC-Derived Sensory Neurons to Evaluate the Effects of External Chemicals on Neuronal Morphology: Possible Implications in the Prediction of Abnormal Skin SensationInternational Journal of Molecular SciencesSeptember 29, 2021Masahiko Satoh, Tamie Suzuki, Tetsuhito Sakurai, Sumika Toyama, Yayoi Kamata, Shinya Kondo, Yasushi Suga, Mitsutoshi Tominaga, Kenji Takamoriperipherin (AB1530; Millipore, Burlington, MA, USA), βIII-tubulin (801201; BioLegend, San Diego, CA, USA; 5666S; Cell Signalling Technology, Danvers, MA, USA), or nicotinamide mononucleotide adenylyltransferase 2

(8) Ndufs4 ablation decreases synaptophysin expression in hippocampusScientific reportsMay 26, 2021Shil SK, Kagawa Y, Umaru BA, Nanto-Hara F, Miyazaki H, Yamamoto Y, Kobayashi S, Suzuki C, Abe T, Owada Y.,Goat anti-mouse IgG-Alexa 488 (1:250)" Rabbit mococlonal anti-β-III Tubulin,IC: 1:00,"Cell Signaling, Cat. No. 5666P",Goat anti-rabbit IgG-Alexa 568 (1:500) Rabbit polyclonal anti EAAT1,WB: 1:1000,"Cell Signaling, Cat.

(9) PRMT5 Promotes EMT Through Regulating Akt Activity in Human Lung CancerCell TransplantationApril 8, 2021Jianhao Huang, Yonghua Zheng, Xiao Zheng, Bao Qian, Qi Yin, Jingjing Lu, Han LeiCollagen I (cat# ab34710; abcam), ZEB1 (cat# 70512; Cell Signaling Technology), and β-tubulin (cat# 5666; Cell Signaling Technology). After incubation, the membranes were washed with TBST and labeled with

(10) PRMT5 regulates colorectal cancer cell growth and EMT via EGFR/Akt/GSK3β signaling cascadesAging (Albany NY)February 15, 2021Yongrong Yan, Peipei Zhao, Zihuan Wang, Zhen Liu, Zhizhi Wang, Jinglan Zhang, Yanqing Ding, Xing Hua, Lina Yu2085; Cell Signaling Technology), β-catenin (cat# 8480; Cell Signaling Technology) and β-tubulin (cat# 5666; Cell Signaling Technology). The membranes were washed and labeled with goat anti-rabbit conjugated

(11) Thyroid and androgen receptor signaling are antagonized by μ‐Crystallin in prostate cancerInternational Journal of CancerFebruary 1, 2021Osman Aksoy et al.dilution of 1:1000. β‐Actin (Cat# 4967, 1:10 000 dilution, Cell Signaling Technology) β‐tubulin (Cat# 5666, 1:10 000 dilution, Cell Signaling Technology). Secondary mouse (315‐0035‐008) and rabbit (111‐036‐047)

(12) Insulin Resistance Promotes Parkinson's Disease through Aberrant Expression of α-Synuclein, Mitochondrial Dysfunction, and Deregulation of the Polo-Like Kinase 2 SignalingCellsMarch 17, 2020Hong CT, Chen KY, Wang W, Chiu JY, Wu D, Chao TY, Hu CJ, Chau KD, Bamodu OA.Subsequent staining of the ADSC-differentiated cells with neuron-specific class III β-tubulin (TuJ-1; Cat. # 5666S, Cell Signaling Technology, Beverly, MA, USA), a neuronal cell marker, and glial fibrillary acidic protein

(13) Engineered nanomedicine for neuroregeneration: light emitting diode-mediated superparamagnetic iron oxide-gold core-shell nanoparticles functionalized by nerve growth factorNanomedicine : nanotechnology, biology, and medicineOctober 1, 2019Yuan M, Wang Y, Qin YX.TBS). After blocking, the membrane was incubated overnight at 4 °C with β3-tubulin (Cell Signaling, 5666) and GAPDH (Invitrogen, PA1988) primary antibodies (at 1:1000), respectively. After washing 5 times

(14) Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axisCell death & diseaseOctober 10, 2018Zhang K, Fu G, Pan G, Li C, Shen L, Hu R, Zhu S, Chen Y, Cui H.DMSO. Anti-Cyclin D1 (2922), anti-CDK4 (12790), anti-CDK6 (2546), anti-GFAP (12389), anti-t3-Tubulin (5666), anti-E-cadherin (14472), anti-Vimentin (5741) and anti-MCM2 (3619) antibodies were obtained from Cell Signalling

(15) miR-182 controls cell growth in gastrointestinal stromal tumors by negatively regulating CYLD expressionOncology ReportsOctober 3, 2018Tianlong Ling, Fengrong Yu, Hui Cao(dilution 1:1,000; cat. no. 8242; Cell Signaling Technology, Inc.), β-tubulin (dilution 1:1,000; cat. no. 5666; Cell Signaling Technology, Inc.) and CYLD (dilution 1:1,000; cat. no. 8462; Cell Signaling Technology,

(16) Mitofusin 2 Regulates Axonal Transport of Calpastatin to Prevent Neuromuscular Synaptic Elimination in Skeletal MusclesCell MetabolismSeptember 1, 2018Luwen Wang et al.RRID: AB_2533071" Rabbit monoclonal anti-βIII-Tubulin (clone D65A4),Cell Signaling Technology,"Cat# 5666S, RRID: AB_10694388" Mouse monoclonal anti-Tau (clone Tau46),Cell Signaling Technology,"Cat# 4019S,

(17) A disease-associated Aifm1 variant induces severe myopathy in knockin miceMolecular metabolismJuly 1, 2018Wischhof L, Gioran A, Sonntag-Bensch D, Piazzesi A, Stork M, Nicotera P, Bano D.(Cell Signaling 4685), rabbit anti-phospho-Akt (Cell Signaling 4058), rabbit anti-βIII tubulin (Cell Signaling 5666), rabbit anti-calbindin (Swant CD 68), rabbit anti-Diablo (Abcam ab8115), rabbit anti-GAPDH (Cell Signalling

(18) Pharmacological TLR4 Antagonism Using Topical Resatorvid Blocks Solar UV-Induced Skin Tumorigenesis in SKH-1 MiceCancer Prevention ResearchMay 1, 2018Karen Blohm-Mangone et al.(9215), total p38 (8690), phospho-Akt (4060), p21waf1 (2946), cleaved caspase 3 (9661) and beta tubulin (5666). The beta actin antibody was purchased from Sigma-Aldrich (A5441, St. Louis, MO), and the TLR4 antibody

(19) MiR-152-5p as a microRNA passenger strand special functions in human gastric cancer cellsInternational Journal of Biological SciencesApril 30, 2018Wendao You et al.probed with the specific primary antibodies against PIK3CA (#4249, Cell Signaling, USA), or Tubulin (#5666, Cell Signaling, USA). Finally, protein bands were visualized using enhanced chemiluminescent substrate,

(20) An unexpected link between fatty acid synthase and cholesterol synthesis in proinflammatory macrophage activationThe Journal of biological chemistryApril 13, 2018Carroll RG, Zasłona Z, Galván-Peña S, Koppe EL, Sévin DC, Angiari S, Triantafilou M, Triantafilou K, Modis LK, O'Neill LA.anti-phospho-TAK1 (catalogue no. 4508), anti-phospho-IKKα/β (catalogue no. 2697), anti-tubulin (catalogue no. 5666), and anti-histone H3 (catalogue no. 4499) all from Cell Signaling Technology. Secondary horseradish

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beta3-Tubulin (D65A4) XP ® Rabbit mAb

beta3-Tubulin (D65A4) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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beta3-Tubulin (D65A4) XP ® Rabbit mAb from Cell Signaling Technology

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