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Antibodies
Anti-ME1 antibody
Anti-ME1 antibody from Abcam
Be the first to write a review!
Citations:
(20)
Supplier Page
Supplier Page from
Abcam for
Anti-ME1 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-ME1 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab97445
Quantity
50 UL
Applications
Immunocytochemistry/ Immunofluorescence, Western blot
Antibody Type
Primary
Conjugate/Tag
Unconjugated
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Concentration
1 mg/ml
Gene Name
Me1
Reactivity
Human
Target
ME1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (20)
(1)
Malic Enzyme 1 Absence in Synovial Sarcoma Shifts Antioxidant System Dependence and Increases Sensitivity to Ferroptosis Induction with ACXT-3102
Clinical Cancer Research
August 15, 2022
Caitlyn B. Brashears, Bethany C. Prudner, Richa Rathore, Katharine E. Caldwell, Carina A. Dehner, Jane L. Buchanan, Sara E.S. Lange, Neal Poulin, Jennifer K. Sehn, Jason Roszik,
et al.
Slides were heated in antigen unmasking solution (Vector). Primary antibody (
1:100
malic enzyme 1,
ab97445
,
Abcam
) was applied to each slide and incubated overnight at 4°C. Biotinylated secondary antibody (
1:100
(2)
Lactate and glutamine support NADPH generation in cancer cells under glucose deprived conditions
Redox Biology
October 1, 2021
Minfeng Ying, Duo You, Xiaobing Zhu, Limeng Cai, Siying Zeng, Xun Hu
used here include: anti-
IDH1
(
1:1000
,
Abcam
, cat. no.
ab172964
), anti-ME1 (
1:2000
,
Abcam
, cat. no.
ab97445
), anti-
MDH1
(
1:10000
,
Abcam
, cat. no.
ab180152
), anti-
Visfatin
(
1:1000
,
Abcam
, cat. no.
ab236874
), anti-LDHA
(3)
Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis
Cell metabolism
May 4, 2021
Wang YP, Sharda A, Xu SN, van Gastel N, Man CH, Choi U, Leong WZ, Li X, Scadden DT.
anti-Flag (
clone
M2),
Sigma-Aldrich
,Cat#:
F1804
; RRID: AB_262044 Rabbit polyclonal anti-ME1,
Abcam
,Cat#:
ab97445
; RRID: AB_10679994 Rabbit monoclonal anti-
ME2
(
clone
EP7217),
Abcam
,Cat#:
ab139686
Rabbit monoclonal
(4)
Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis
Redox biology
September 1, 2020
Shao C, Lu W, Du Y, Yan W, Bao Q, Tian Y, Wang G, Ye H, Hao H.
antibodies used in this study include antibodies against ME1 (
1:1000
, 5% non-fat milk,
Abcam
, cat. no.
ab97445
), G6PD (
1:1000
, 5% non-fat milk,
Abcam
, cat no.
ab993
), β-actin (
1:1000
, 5% BSA,
Cell Signaling Technology
,
(5)
A small molecule G6PD inhibitor reveals immune dependence on pentose phosphate pathway
Nature chemical biology
July 1, 2020
Ghergurovich JM, García-Cañaveras JC, Wang J, Schmidt E, Zhang Z, TeSlaa T, Patel H, Chen L, Britt EC, Piqueras-Nebot M,
et al
.
Anti-β-actin (
5125
) was obtained from
Cell Signaling Technologies
(Danvers, MA). Anti-G6PD (
ab993
); ME1 (
ab97445
) and IDH1 (EPR12296) were obtained from
Abcam
Inc. (Cambridge, MA).
CoxIV antibody
was obtained from
(6)
CMap analysis identifies Atractyloside as a potential drug candidate for type 2 diabetes based on integration of metabolomics and transcriptomics
Journal of Cellular and Molecular Medicine
July 1, 2020
Hailong Li, Xiaodong Shi, Hua Jiang, Junren Kang, Miao Yu, Qifei Li, Kang Yu, Zhengju Chen, Hui Pan, Wei Chen
PVDF
membranes
were incubated with the primary antibody at 4°C overnight, including anti‐ME1 (
1:1000
,
ab97445
,
Abcam
), anti‐
ME2
(
1:2000
,
ab139686
,
Abcam
), and anti‐
MDH1
(
1:1000
,
ab181091
,
Abcam
), with β‐actin (
1:200
,
(7)
Malic Enzyme 1 Indicates Worse Prognosis in Breast Cancer and Promotes Metastasis by Manipulating Reactive Oxygen Species
OncoTargets and therapy
January 1, 2020
Liu C, Cao J, Lin S, Zhao Y, Zhu M, Tao Z, Hu X.
Buffer (Beyotime, P0260, China) for 15 min, the sections were incubated at 4°C with ME1 antibody (
Abcam
,
ab97445
,
1:1000
, USA) overnight. The
IHC
Kit (GTVision, GK500705, China) including second antibody and DAB substrate
(8)
Malic enzyme 1 (ME1) is a potential oncogene in gastric cancer cells and is associated with poor survival of gastric cancer patients
OncoTargets and Therapy
July 11, 2019
Yanyan Shi, Siliang Zhou, Pan Wang, Yanlei Guo, Bingteng Xie, Shigang Ding
(forward primer) and 5ʹ- CTTGTTCAGGAGACGAAATGC-3ʹ (reverse primer). Primary antibodies for ME1 (
1:500
,
ab97445
,
Abcam
, Cambridge, MA, USA) and β-actin (
1:1000
,
ab8227
,
Abcam
, Cambridge, MA, USA) were used
for Western blot
(9)
Metabolic reprogramming and Notch activity distinguish between non-small cell lung cancer subtypes
British journal of cancer
July 1, 2019
Sellers K, Allen TD, Bousamra M 2nd, Tan J, Méndez-Lucas A, Lin W, Bah N, Chernyavskaya Y, MacRae JI, Higashi RM,
et al
.
ab135532
,
Abcam
) PSAT (
1:500
, 20180-1-AP, Protein Tech) PHGDH (
1:1000
,
sc292792
,
Santa Cruz
) ME1 (
1:1000
,
ab97445
,
Abcam
)
ME2
(
1:500
,
ab139686
,
Abcam
) GOT1 (
1:250
,
ap2947a
,
Abgent
) GOT2 (
1:500
,
ab93928
,
Abcam
) IDH1
(10)
NADPH production by the oxidative pentose-phosphate pathway supports folate metabolism
Nature metabolism
March 1, 2019
Chen L, Zhang Z, Hoshino A, Zheng HD, Morley M, Arany Z, Rabinowitz JD.
Flag-tag (
2368
) were obtained from
cell Signaling Technologies
(Danvers, MA). Anti-G6PD (
ab993
), ME1 (
ab97445
), IDH1 (EPR12296) and PGD (EPR6565) were obtained from
Abcam
Inc. (Cambridge, MA). Anti-
DHFR
(377091)
(11)
HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma
Journal of cellular and molecular medicine
November 1, 2017
Li W, Chen C, Zhao X, Ye H, Zhao Y, Fu Z, Pan W, Zheng S, Wei L, Nong T,
et al
.
anti‐
human
GLUD1
antibody (
1:1000
, #
ab168352
,
Abcam
, Cambridge, MA), rabbit anti‐human ME1 antibody(
1:1000
, #
ab97445
,
Abcam
, Cambrigde, MA), mouse anti‐human MDH1 antibody(
1:500
, #
ab76616
, Cambrigde, MA) rabbit anti‐
human
(12)
Cancer-Associated IDH1 Promotes Growth and Resistance to Targeted Therapies in the Absence of Mutation
Cell Reports
May 27, 2017
Andrea E. Calvert et al.
CS200603), anti-H3K36me3 (
Abcam
,
ab9050
), anti-Histone H3 (
Cell Signaling
,
4499
), anti-ME1 (
Abcam
,
ab97445
),
anti-FoxO6
(
Thermo Scientific
PA5–35117
), anti-phospho-
FoxO6
(
Abcam
,
ab154832
), anti-phospho-EGFR
(13)
Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage
Nature chemical biology
May 1, 2016
Liu L, Shah S, Fan J, Park JO, Wellen KE, Rabinowitz JD.
9b,c. The antibodies to the following proteins were purchased from the indicated sources: ME1 (
Abcam
,
ab97445
,
1:1,000
dilution),
ME2
(
Abcam
,
ab139686
,
1:1,000
dilution),
ME3
(
Abcam
,
ab172972
,
1:2,000
dilution),
(14)
The HMGB1 protein induces a metabolic type of tumour cell death by blocking aerobic respiration
Nature communications
March 7, 2016
Gdynia G, Sauer SW, Kopitz J, Fuchs D, Duglova K, Ruppert T, Miller M, Pahl J, Cerwenka A, Enders M,
et al
.
(15)
Activated mTORC1 promotes long-term cone survival in retinitis pigmentosa mice
Journal of Clinical Investigation
April 1, 2015
Aditya Venkatesh, Shan Ma, Yun Z. Le, Michael N. Hall, Markus A. Rüegg, Claudio Punzo
(Ser422) (
1:300
; catalog
ab55281
); rabbit α-G6PD (
1:300
; catalog
ab993
); rabbit α-ME1 (
1:300
; catalog
ab97445
); rabbit α-Ki67 (
1:300
; catalog
ab15580
) (all from
Abcam
); rabbit α–HIF-1α (
1:300
; catalog ab1536; R&D
(16)
Metabolic efficiency promotes protection from pressure overload in hearts expressing slow skeletal troponin I
Circulation. Heart failure
January 1, 2015
Carley AN, Taglieri DM, Bi J, Solaro RJ, Lewandowski ED.
chain, M8424-0.2ML Sigma Aldrich; calsequestrin,
PA1-913
Pierce
Thermo Scientific; malic enzyme 1,
ab97445
Abcam
; phospho-AMP kinase α,
2535S
Cell Signaling
; pyruvate dehydrogenase,
ab110330
Abcam
; phosphorylated
(17)
Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice
PloS one
January 1, 2014
Al-Dwairi A, Brown AR, Pabona JM, Van TH, Hamdan H, Mercado CP, Quick CM, Wight PA, Simmen RC, Simmen FA.
nitrocellulose
membrane
.
Membranes
were incubated overnight with rabbit polyclonal antibody raised against
ME1
(
ab97445
, 1∶1000 dilution;
Abcam
, Cambridge, MA), rabbit polyclonal antibody raised against FASN (
ab22759
, 1∶1000
(18)
Metabolic intervention on lipid synthesis converging pathways abrogates prostate cancer growth
Oncogene
October 17, 2013
Fritz V, Benfodda Z, Henriquet C, Hure S, Cristol JP, Michel F, Carbonneau MA, Casas F, Fajas L.
(19)
Dietary soy protein induces hepatic lipogenic enzyme gene expression while suppressing hepatosteatosis in obese female Zucker rats bearing DMBA-initiated mammary tumors
Genes & nutrition
October 1, 2012
Reza Hakkak, Ahmed Al-Dwairi, George J Fuchs, Soheila Korourian, Frank A Simmen
(20)
Cytosolic malic enzyme 1 (ME1) mediates high fat diet-induced adiposity, endocrine profile, and gastrointestinal tract proliferation-associated biomarkers in male mice
PloS one
January 1, 2012
Al-Dwairi A, Pabona JM, Simmen RC, Simmen FA.
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Anti-ME1 antibody from Abcam
Supplier Page
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Pricing Info
Product Specs
Company Info
Citations
Reviews
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