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Anti-Thrombospondin 1 antibody
Anti-Thrombospondin 1 antibody from Abcam
Be the first to write a review!
Citations:
(47)
Supplier Page
Supplier Page from
Abcam for
Anti-Thrombospondin 1 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Thrombospondin 1 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
ab85762
Quantity
100 UG
Antibody Type
Primary
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/Tag
Unconjugated
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Concentration
0.5 mg/ml
Gene Name
THBS1
Target
Thrombospondin 1
Reactivity
Human (Hu)
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (47)
(1)
Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury
bioRxiv : the preprint server for biology
February 8, 2023
Mohr ME, Li S, Trouten AM, Stairley RA, Roddy PL, Liu C, Zhang M, Sucov HM, Tao G.
Developmental Studies Hybridoma Bank, AB_2147781), Vimentin (
1:400
,
Abcam
,
ab92547
), TSP1 (
1:100
,
Abcam
,
ab85762
), and cTNT (
1:400
, Fisher, ms-295p1abx). Secondary antibodies used include: Goat Anti-Mouse IgG 488
(2)
Thrombospondin 1 missense alleles induce extracellular matrix protein aggregation and TM dysfunction in congenital glaucoma
The Journal of clinical investigation
December 1, 2022
Fu H, Siggs OM, Knight LS, Staffieri SE, Ruddle JB, Birsner AE, Collantes ER, Craig JE, Wiggs JL, D'Amato RJ.
BSA, and 0.5% Triton X-100 in 0.1 M PB at room temperature for 3 hours. Anti-THBS1 antibody (catalog
ab85762
,
Abcam
) was used as the primary antibody (5 μg/mL). After 3 days of
incubation
at 4°C, the samples were
(3)
A BDNF-TrkB autocrine loop enhances senescent cell viability
Nature Communications
October 20, 2022
Carlos Anerillas, Allison B. Herman, Rachel Munk, Amanda Garrido, Kwan-Wood Gabriel Lam, Matthew J. Payea, Martina Rossi, Dimitrios Tsitsipatis, Jennifer L. Martindale, Yulan Piao,
et al.
sc-377218
),
DPP4
(
1:100
,
Cell Signaling Technology
67138
), FN1 (
1:50
,
Abcam
ab2413
), and THBS1 (
1:50
,
Abcam
ab85762
). Fluorescent signals were detected by adding fluorescent secondary antibodies [
Invitrogen
,
Goat anti-Mouse IgG (H + L) Cross-Adsorbed
(4)
A Novel GATA1 Variant in the C-Terminal Zinc Finger Compared with the Platelet Phenotype of Patients with A Likely Pathogenic Variant in the N-Terminal Zinc Finger
Cells
October 14, 2022
José M. Bastida, Stefano Malvestiti, Doris Boeckelmann, Verónica Palma-Barqueros, Mira Wolter, MarÃa L. Lozano, Hannah Glonnegger, RocÃo Benito, Carlo Zaninetti, Felix Sobotta,
et al.
protein ß-tubulin was determined. The following primary antibodies were used: anti-thrombospondin (
ab85762
,
Abcam
, Cambridge, UK); anti-P-selectin (
555522
,
BD Biosciences
, San Jose, CA, USA); anti-vWF (A0082,
(5)
Src-related thrombocytopenia: a fine line between a megakaryocyte dysfunction and an immune-mediated disease
Blood Advances
September 13, 2022
Verónica Palma-Barqueros, Nuria Revilla, Carlo Zaninetti, Ana Maria Maria Galera, Ana Sánchez-Fuentes, Ana Zámora-Cánovas, Natalia Bohdan, José Padilla, Ana MarÃn-Quilez, AgustÃn Rodriguez-Alen,
et al.
were performed on blood smears as reported30 using specific antibodies (anti-thrombospondin antibody [
ab85762
,
Abcam
, Cambridge, UK], anti-P-Selectin [
555522
,
BD Biosciences
, San Jose, CA], anti-VWF [A0082, Dako,
(6)
Oncogenic collagen I homotrimers from cancer cells bind to α3β1 integrin and impact tumor microbiome and immunity to promote pancreatic cancer
Cancer cell
August 8, 2022
Chen Y, Yang S, Tavormina J, Tampe D, Zeisberg M, Wang H, Mahadevan KK, Wu CJ, Sugimoto H, Chang CC,
et al
.
RRID:AB_2811309"
Goat
Collagen IV,
Abcam
,"Cat#
ab6585
, RRID:AB_305583" Rabbit Thrombospondin-1,
Abcam
,"Cat#
ab85762
, RRID:AB_10674322" Rabbit
Laminin
,
Sigma-Aldrich
,"Cat#
L9393
, RRID:AB_477163" Rabbit FSP1,DAKO,"Cat#
(7)
Collagen VII maintains proteostasis in dermal fibroblasts by scaffolding TANGO1 cargo
Matrix biology : journal of the International Society for Matrix Biology
August 1, 2022
Cao Q, Tartaglia G, Alexander M, Park PH, Poojan S, Farshchian M, Fuentes I, Chen M, McGrath JA, Palisson F,
et al
.
antibodies were used at the following dilutions:
TSP1
(
sc-59887
, Santa Cruz Biotechnology,
Mouse
,
1:1000
or
ab85762
,
Abcam
, rabbit,
1:2000
) or (
ab85762
, Abcam, rabbit,
1:2000
),
TGM2
(
sc-48387
,
Santa Cruz
,
mouse
,
1:1000
)
(8)
A TMT-based shotgun proteomics uncovers overexpression of thrombospondin 1 as a contributor in pyrrolizidine alkaloid-induced hepatic sinusoidal obstruction syndrome
Archives of Toxicology
July 1, 2022
Weiqian Wang et al.
3 mg of LSKL per mL for
animal experiments
. Primary antibodies, including thrombospondin 1 (TSP1, #
ab85762
), matrix metallopeptidase 9 (
MMP9
, #
ab228402
), transforming growth factor-beta 1 (TGFB1, #
ab179695
),
(9)
Versican promotes T helper 17 cytotoxic inflammation and impedes oligodendrocyte precursor cell remyelination
Nature communications
May 4, 2022
Ghorbani S, Jelinek E, Jain R, Buehner B, Li C, Lozinski BM, Sarkar S, Kaushik DK, Dong Y, Wight TN,
et al
.
(Amsbio, 370255), fibronectin (
Abcam
,
ab23750
), fibrinogen (
Abcam
,
ab34269
), thrombospondin-1 (
Abcam
,
ab85762
), myelin basic protein (MBP,
Abcam
,
ab7349
), Olig2 (
Millipore
,
ab9610
),
platelet
-derived growth factor
(10)
Prioritization of Candidate Biomarkers for Degenerative Aortic Stenosis through a Systems Biology-Based In-Silico Approach
Journal of Personalized Medicine
April 15, 2022
Nerea Corbacho-Alonso, Tamara Sastre-Oliva, Cecilia Corros, Teresa Tejerina, Jorge Solis, Luis F. López-Almodovar, Luis R. Padial, Laura Mourino-Alvarez, Maria G. Barderas
non-fat dry milk. The primary antibodies used were antisera against thrombospondin-1 (THBS, 1/100,
Abcam
ab85762
, Cambridge, UK), endoplasmin (GRP94, 1/100,
Abcam
ab3674
, Cambridge, UK), and α-
smooth muscle
actin
(11)
Single-cell RNA sequencing identifies a migratory keratinocyte subpopulation expressing THBS1 in epidermal wound healing
iScience
April 15, 2022
Siriwach R, Ngo AQ, Higuchi M, Arima K, Sakamoto S, Watanabe A, Narumiya S, Thumkeo D.
0.3% Triton X-100. Cells were incubated with primary antibodies for
FLG
(
1:200
),
THBS1
(
1:500
(
Abcam
,
ab85762
) or
1:250
(
Invitrogen
,
MA5-13398
)), MAP kinase, activated (
1:50
), or Ki67 (
1:500
) overnight at 4°C and
(12)
Unravelling Novel Roles of Salivary Exosomes in the Regulation of Human Corneal Stromal Cell Migration and Wound Healing
International Journal of Molecular Sciences
April 14, 2022
Paulina Escandon, Angela Liu, Sarah E. Nicholas, Asher Khan, Kamran M. Riaz, Dimitrios Karamichos
dilution of a primary antibody including EDA-Fn (
SAB4200784
;
Millipore Sigma
, Burlington, MA, USA), TSP-1 (
ab85762
;
Abcam
, Cambridge, MA, USA), and α-SMA (
ab5694
;
Abcam
, Cambridge, MA, USA), β-actin (
ab184092
;
Abcam
,
(13)
Augmentation of CD47/SIRPα signaling protects cones in genetic models of retinal degeneration
JCI Insight
August 23, 2021
Sean K. Wang, Yunlu Xue, Constance L. Cepko
temperature and incubated with
1:1000
of anti-CD47 (
BD Biosciences
,
555297
),
1:500
of anti-TSP1 (
Abcam
,
ab85762
), or
1:500
of anti-SIRPα (
QED Bioscience
,
2428
) in blocking solution overnight at 4°C. Sections were
(14)
Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia
STAR protocols
December 18, 2020
Ryan SK, Jordan-Sciutto KL, Anderson SA.
(
1:250
),
Abcam
,
ab185230
Synaptophysin
clone
SY38 (
mouse
) (
1:250
),Millipore,MAB5258 Thrombospondin-1 (rabbit) (
1:250
),
Abcam
,
ab85762
TMEM119
(rabbit),
Abcam
,
ab185333
,, Bacterial and
Virus
Strains
,Bacterial and
Virus
Strains
,Bacterial
(15)
Segmental differences found in aqueous angiographic-determined high - and low-flow regions of human trabecular meshwork
Experimental Eye Research
July 1, 2020
Sindhu Saraswathy, Thania Bogarin, Ernesto Barron, Brian A. Francis, James C.H. Tan, Robert N. Weinreb, Alex S. Huang
(
Abcam
:
ab19345
, polyclonal
1:500
), MMP3 (Antibody Verify: AAS41420C, polyclonal
1:100
), TSP-1 (
Abcam
:
ab85762
, polyclonal
1:500
), TGFβ2 (
Abcam
:
ab36495
, monoclonal
1:500
), TGFβR1 (
Abcam
:
ab31013
, polyclonal
1:500
),
(16)
H19/TET1 axis promotes TGF-β signaling linked to endothelial-to-mesenchymal transition
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
June 1, 2020
Cao T, Jiang Y, Li D, Sun X, Zhang Y, Qin L, Tellides G, Taylor HS, Huang Y.
immunofluorescence [IF
]),
TGFBR2
(
Abcam
,
ab184948
; used at a dilution of 1/1000
in WB
), TSP1 (
Abcam
,
ab85762
; used at a dilution of 1/500
in WB
), pSMAD2 (
Cell Signaling
,
3101
; used at a dilution of 1/500
in WB
),
(17)
Gastric Cancer Cell-Derived Exosomal microRNA-23a Promotes Angiogenesis by Targeting PTEN
Frontiers in Oncology
March 13, 2020
Jiang Du, Yuan Liang, Ji Li, Jin-Ming Zhao, Zhen-Ning Wang, Xu-Yong Lin
(VEGF;
1:1,000
, AV202-1, Beyotime Institute of Biotechnology, China), thrombospondin-1 (TSP-1;
1:10,000
,
ab85762
,
Abcam
Inc., Cambridge, UK) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH;
1:10,000
,
ab181602
,
(18)
The Effect of 3′-Hydroxy-3,4,5,4′-Tetramethoxy -stilbene, the Metabolite of the Resveratrol Analogue DMU-212, on the Motility and Proliferation of Ovarian Cancer Cells
International Journal of Molecular Sciences
February 7, 2020
Andrzej Nowicki, Paulina Skupin-Mrugalska, Malgorzata Jozkowiak, Marcin Wierzchowski, Marcin Rucinski, Piotr Ramlau, Violetta Krajka-Kuzniak, Jadwiga Jodynis-Liebert, Hanna Piotrowska-Kempisty
USA), rabbit polyclonal anti-SMAD7 Ab (
ab90086
,
Abcam
, Cambridge, UK), rabbit polyclonal anti-THBS1 (
ab85762
,
Abcam
), rabbit polyclonal anti-
GPR56
Ab (
ab172361
,
Abcam
) and rabbit polyclonal anti-RGC32 Ab (
sc-84222
,
(19)
A Positive Feedback Loop of TET3 and TGF-β1 Promotes Liver Fibrosis
Cell reports
February 4, 2020
Xu Y, Sun X, Zhang R, Cao T, Cai SY, Boyer JL, Zhang X, Li D, Huang Y.
,
Millipore Sigma
,Cat#
ABE290
Anti-TGF-β1,
Abcam
,Cat#
ab92486
Anti-
TGFBR2
,
Abcam
,Cat#
ab186838
Anti-TSP1,
Abcam
,Cat#
ab85762
Anti-SMAD3,Cell Signaling,Cat# C67H9 Anti-phospho-SMAD3,Cell Signaling,Cat# C25A9 Anti-
COL1A1
,
Santa Cruz
(20)
TGFβ2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells
Nature communications
January 23, 2020
Corbet C, Bastien E, Santiago de Jesus JP, Dierge E, Martherus R, Vander Linden C, Doix B, Degavre C, Guilbaud C, Petit L,
et al
.
1:1000
),
HRP-conjugated streptavidin
(#
SA10001
;
Thermo Fisher Scientific
;
1:25,000
) and anti-TSP1 (
ab85762
;
Abcam
;
1:1000
) antibodies were also applied in 5% BSA overnight at 4 °C. In some experiments, cell
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Anti-Thrombospondin 1 antibody from Abcam
Supplier Page
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Company Info
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Reviews
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