Anti-NG2 antibody ab83178 from Abcam

Product Specs
ItemAnti-NG2 antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab83178
Quantity100 UG
HostRabbit
Conjugate/TagUnconjugated
Antibody TypePrimary
IsotypeIgG
ApplicationsImmunohistochemistry (PFA perfusion fixed frozen sections), Western blot
ClonalityPolyclonal
Concentration0.4 mg/ml
Gene NameCSPG4
ValidationKnockOut Validated
ReactivityHuman, Rat
TargetNG2

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature OligodendrocytesCellular and molecular neurobiologyJuly 1, 2023Ye D, Wang Q, Yang Y, Chen B, Zhang F, Wang Z, Luan Z.Cat. #MAB2864, R&D Systems, Minneapolis, MN, USA, RRID: AB_2195180), rabbit anti-NG2 (1:100; Cat. #ab83178, Abcam, Cambridge, UK, RRID: AB_10672215), and rabbit anti-PDGFR-α (1:800; Cat. #C5241, Cell Signaling

(2) Protocol to generate endothelial cells, pericytes, and fibroblasts in one differentiation round from human-induced pluripotent stem cellsSTAR ProtocolsJune 1, 2023Tea Soon Park, Rishabh Hirday, Amir Ali, Roba Megersa, Rafael Villasmil, Eric Nguyen, Kapil Bharti,Thermo Fisher Scientific,H3570 Dil-Ac-LDL (5–15 μg/mL),Thermo Fisher Scientific,L3484 NG2 (0.5 mg/mL),Abcam,ab83178 aSMA-FITC (1:200),Sigma-Aldrich,F3777 Collagen-I (1:100),Thermo Fisher Scientific,PA5-95137 Vimentin

(3) Discovery of Hippo signaling as a regulator of CSPG4 expression and as a therapeutic target for Clostridioides difficile diseasePLOS PathogensMarch 27, 2023Jason L. Larabee, D. Annie Doyle, Ummey Khalecha Bintha Ahmed, Tyler M. Shadid, Rachel R. Sharp, Kenneth L. Jones, Young Mi Kim, Shibo Li, Jimmy D. Ballardagainst E-cadherin (Santa Cruz Biotechnology; sc-59778 AF488) and rabbit antibodies against CSPG4 (Abcam; ab83178). After 3 washes, the sections were incubated with a Cy3 labeled goat anti-rabbit antibody (Abcam; ab6939)

(4) C-X-C chemokine receptor type 7 antibody enhances neural plasticity after ischemic strokeNeural Regeneration ResearchMarch 17, 2023Shan-Shan Zhang, Xiao-Qian Zhang, Bing-Chao Wang, Bing-Chao Dong, Mei-Xuan Wang, Ting Wang, Shan-Shan Xiao1:500, Abcam, Cat# ab12274, AB_2247459), rabbit anti-neuron-glial antigen 2 (NG2; 1:200, Abcam, Cat# ab83178, RRID: AB_10672215), rabbit anti-Olig2 (1:500, Abcam, Cat# ab109186, RRID: AB_10861310), mouse anti-myelin

(5) A microengineered Brain-Chip to model neuroinflammation in humansiScienceAugust 19, 2022Pediaditakis I, Kodella KR, Manatakis DV, Le CY, Barthakur S, Sorets A, Gravanis A, Ewart L, Rubin LL, Manolakos ES, et al.(Abcam; 32127), GFAP (Abcam; ab53554), GLAST (Invitrogen; PA5-19709), s100β (Abcam; 52642), NG2 (Abcam; ab83178), αSMA (Abcam; 7817), IBA1 (FUJIFILM; 019-19741), CD68 (Abcam; ab213363), ICAM-1 (R&D Systems; BBA3),

(6) Identifying the functions of two biomarkers in human oligodendrocyte progenitor cell developmentJournal of Translational MedicineDecember 1, 2021Haipeng Zhou, Ying He, Zhaoyan Wang, Qian Wang, Caiyan Hu, Xiaohua Wang, Siliang Lu, Ke Li, Yinxiang Yang, Zuo LuanMonoclonal mouse anti-PDGFR-α (1:250, Cat. #C2318, CST, Boston, MA, USA), rabbit anti-NG2 (1:50, Cat. #ab83178, Abcam, Cambridge, Cambridgeshire, UK) and mouse anti-A2B5 (1:50, Cat. #MAB1416, R&D Systems, Minneapolis,

(7) Olfactory ensheathing cell transplantation alters the expression of chondroitin sulfate proteoglycans and promotes axonal regeneration after spinal cord injuryNeural Regeneration ResearchJanuary 12, 2021Xi-Jing Wang, Zhi-Jian Cheng, Zhi-Jian Cheng, Hao-Peng Yuan, Hao-Peng LiBioss (Cat# bs-0154R; Beijing, China), rabbit anti-NG2 antibody (1:200) was purchased from Abcam (Cat# ab83178; Cambridge, MA, USA), rabbit anti-neurocan antibody (1:200) was purchased from Millipore (Cat# ABT1382;

(8) Spatial heterogeneity of cell-matrix adhesive forces predicts human glioblastoma migrationNeuro-Oncology AdvancesAugust 15, 2020Rasha Rezk, Bill Zong Jia, Astrid Wendler, Ivan Dimov, Colin Watts, Athina E Markaki, Kristian Franze, Alexandre J Kablaantibodies were used: Nestin (Abcam, ab22035, 1:100), Vimentin (Abcam, ab8069, 1:200), NG2 (Abcam, ab83178, 1:200), Ki67 (Abcam, ab16667, 1:200), Vinculin (FAK100, Sigma 1:200), and F-actin (FAK100, Sigma 1:500).

(9) Acute Cellular and Functional Changes With a Combinatorial Treatment of Ion Channel Inhibitors Following Spinal Cord InjuryFrontiers in Molecular NeuroscienceJune 25, 2020Ryan L. O’Hare Doig, Sreya Santhakumar, Brooke Fehily, Sushmitha Raja, Tanya Solomon, Carole A. Bartlett, Melinda Fitzgerald, Stuart I. Hodgetts1:500; #sc-9974, Santa Cruz Biotechnology, Dallas, TX, USA) and neural/glial antigen 2 (NG2; 1:500; #ab83178, Abcam, Cambridge, UK); lipid peroxidation markers, acrolein (1:500; #ab37110, Abcam, Cambridge, UK);

(10) Tissue-Resident PDGFRα⁺ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent MannerCell reportsJanuary 14, 2020Santini MP, Malide D, Hoffman G, Pandey G, D'Escamard V, Nomura-Kitabayashi A, Rovira I, Kataoka H, Ochando J, Harvey RP, et al.DETAILS Antibodies For immunofluorescence we used the following antibodies against: NG2 (Abcam, ab83178, ab50009 or ab83508), PDGFRα (Santa Cruz, #sc-338 or sc-398206), αSMA-Cy3 (Sigma #C6198), GFP-FITC (Abcam,

(11) Deficiency of Mitochondrial Aspartate-Glutamate Carrier 1 Leads to Oligodendrocyte Precursor Cell Proliferation Defects Both In Vitro and In VivoInternational Journal of Molecular SciencesSeptember 11, 2019Sabrina Petralla et al.(Santa Cruz Biotechnology Cat# sc-48817, RRID:AB_2157550, Lot# C1413, 1:500 dilution), anti-NG2 (Abcam Cat# ab83178, RRID:AB_10672215, Lot# GR3194358-1, 1:500 dilution), anti-PDGFRα (Santa Cruz Biotechnology Cat# sc-338,

(12) Transplantation of human endometrial perivascular cells with elevated CYR61 expression induces angiogenesis and promotes repair of a full-thickness uterine injury in ratStem cell research & therapyJune 18, 2019Li Z, Yan G, Diao Q, Yu F, Li X, Sheng X, Liu Y, Dai Y, Zhou H, Zhen X, et al.stained with primary antibodies such as anti-PDGFRβ antibody (ab139406, Abcam), anti-NG2 antibody (ab83178, Abcam), and anti-α-SMA antibody (ab5694, Abcam) at 4 °C overnight. Secondary Alexa Fluor 488-conjugated

(13) TRIM9-Mediated Resolution of Neuroinflammation Confers Neuroprotection upon Ischemic Stroke in MiceCell reportsApril 9, 2019Zeng J, Wang Y, Luo Z, Chang LC, Yoo JS, Yan H, Choi Y, Xie X, Deverman BE, Gradinaru V, et al.Mouse monoclonal anti-SMI-312 (Abcam, catalog # ab24574), Rabbit polyclonal anti-NG2 (Abcam, catalog # ab83178), Mouse monoclonal anti-Olig2 (Abcam, catalog # MABN50), Mouse monoclonal anti-Flag (Sigma, catalog

(14) Conventional Anti-glioblastoma Chemotherapy Affects Proteoglycan Composition of Brain Extracellular Matrix in Rat Experimental Model in vivoFrontiers in PharmacologyOctober 2, 2018Alexandra Y. Tsidulko, Cynthia Bezier, Gabin de La Bourdonnaye, Anastasia V. Suhovskih, Tatiana M. Pankova, Galina M. Kazanskaya, Svetlana V. Aidagulova, Elvira V. Grigorievawere incubated with anti-decorin (Abcam ab175404), anti-syndecan-1 (Abcam ab34164), anti-NG2 (Abcam ab83178), anti-HS (Millipore MAB2040), and anti-CS (Sigma-Aldrich C8035) primary antibodies for 1 h at room

(15) Astrocytes Control Circadian Timekeeping in the Suprachiasmatic Nucleus via Glutamatergic SignalingNeuronMarch 22, 2017Brancaccio M, Patton AP, Chesham JE, Maywood ES, Hastings MH.Goat polyclonal to EAAC1",Millipore,AB1520; RRID: AB_90732 "Anti-Ng2, Rabbit polyclonal to Ng2",Abcam,ab83178; RRID: AB_10672215 "Anti-Iba1, Rabbit polyclonal to Iba1",Wako Chemicals,019-19741; RRID: AB_839504 "Anti-AVP,

(16) Altered ratios of pro- and anti-angiogenic VEGF-A variants and pericyte expression of DLL4 disrupt vascular maturation in infantile haemangiomaThe Journal of PathologyJune 1, 2016Xi Ye, Yassir Abou-Rayyah, Joyce Bischoff, Alison Ritchie, Neil J Sebire, Patrick Watts, Amanda J Churchill, David O BatesVEGF‐AXXXa 16; CD31, Abcam ab28364; DLL4, Abcam ab183532; human CD31, Cell Signaling 3528; and NG2, Abcam ab83178) were incubated overnight at 4 °C. Appropriate AlexaFluor secondary antibodies (Molecular Probes) were

(17) Umbilical Cord Blood-Derived Mononuclear Cells Exhibit Pericyte-Like Phenotype and Support Network Formation of Endothelial Progenitor Cells In VitroAnnals of Biomedical EngineeringOctober 1, 2015Erica B. Peters, Betty Liu, Nicolas Christoforou, Jennifer L. West, George A. Truskeysc-432, Santa Cruz, CA, USA), α-SMA (2 μg/mL, Abcam, ab7817, Cambridge, MA, USA), NG2 (6 μg/mL, Abcam, ab83178), ephrin-B2 (10 μg/mL, Abcam, ab131536) VE-cadherin (2 μg/mL, Santa Cruz Biotechnology, sc-6458), eNOS

(18) The systemic iron-regulatory proteins hepcidin and ferroportin are reduced in the brain in Alzheimer’s diseaseActa Neuropathologica CommunicationsDecember 1, 2013Animesh Alexander Raha, Radhika Anand Vaishnav, Robert Paul Friedland, Adrian Bomford, Ruma Raha-Chowdhury(monoclonal) 1:500 for IHC Abcam (ab83508) (0.5 mg/ml) Anti-NG2 Rabbit (polyclonal) 1:500 for IHC Abcam (ab83178) (0.4 mg/ml) Anti-GFAP Mouse (monoclonal) 1:1000 for IHC Sigma (G3893 Clone G-A-5) Anti-GFAP Rabbit

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Anti-NG2 antibody from Abcam

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Anti-NG2 antibody from Abcam

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