Fig 1: The mTORC1-dependent GRASP55 proximome(A) Experimental outline of the APEX2-based GRASP55 proximome assay (details in text).(B) CC GO analysis using proteins enriched in the GRASP55 proximome in DMSO-treated WI-26 cells. The color of each box represents fold change values for each protein in rapamycin- versus DMSO-treated cells. The number of proteins in the selected dataset for each term is shown on the right side of each bar.(C) Volcano plot showing all proteins identified in the GRASP55 proximome experiment (gray dots). Proteins used in (B) are shown in blue. Proteins within this subset that belong to the CC GO term “Golgi membrane” are shown with black outline.(D) As in (B), but for proteins enriched in the GRASP55 proximome in rapamycin-treated cells.(E–G) Volcano plots as in (C), but for proteins used in (D) (red dots). Proteins that belong to the CC GO terms “membrane-bounded vesicle” (E), “actin cytoskeleton” (F), or “anchoring junction” (G) are shown with black outline.(H) CoIP experiments in WI-26 cells ± Rapa confirm interaction of GRASP55 with selected proteins from the proximome assays.(I–M) Colocalization analysis of GRASP55 with TMF1 (I), USO1 (J), GOLGIN-45 (K), SCAMP3 (L), and TMEM59-Myc (M) in WI-26 cells ± Rapa. Scale bars: 10 µm.See also Figure S6 and Tables S1, S2, and S3.
Supplier Page from Proteintech Group Inc for TMF1-Specific antibody