Fig 1: Validation of DEPs identified from the comparisons of the N, L and LP groups. (A) Western blot analysis of proteins including THBS4, THBS1, IL1RL1, GLUL and TIMP1. With the exception of the IL1RL1 band, all bands on the Western blot were consistent with the bioinformatic analysis results. RT-qPCR analysis of the following genes: THBS4 (B), THBS1 (C), IL1RL1 (D), GLUL (E) and TIMP1 (F). The mRNA levels of Thbs4, Thbs1, IL1RL1, and Glul were consistent with the trends observed in the bioinformatic analyses. The data are shown as the mean ± SD values; *p < 0.05, **p < 0.01, and ***p < 0.001.
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