Fig 1: RBPs were highly expressed in the FAD NCC models in vitro. (A) The validation of GEO dataset GSE3548 data by real-time PCR. The expressions of HNRNPM, HNRNPC, LARP6, and TRMT1 were significantly up-regulated in FAF-group H9-NCCs compared to control-group H9-NCCs (i). The expressions of PPIL4 and RCAN2 were significantly up-regulated in the shRFC-group H9-NCCs compared to Scramble-group H9-NCCs. (B) Western blots showed evidence of up-regulation of LARP6, hnRNPC and TRMT1 proteins in the FAF-group H9-NCCs (i) and PPIL4 and RCAN2 proteins in the shRFC-group H9-NCCs. n=3. * P<0.05, ** P<0.01. FAD, folic acid deficiency; RFC, reduced folate carrier; hnRNPM, heterogeneous nuclear ribonucleoprotein M; hnRNPC, heterogeneous nuclear ribonucleoprotein C; LARP6, La ribonucleoprotein domain family member 6; TRMT1, tRNA methyltransferase 1; PPIL4, peptidylprolyl isomerase like 4; RCAN2, regulator of calcineurin 2.