Fig 2: A high density of macrophages surrounds Plasmodium infected hepatocytes. (A) Representative image of CLEC4F+ staining within an infected liver. Nuclear DAPI staining is shown in blue, CLEC4F in green. Ring1 and Ring2 outer boundaries are indicated by dashed white circles. (B) CLEC4F staining of a single cell within Ring2 (C) Representative image of a CLEC4F+ cell in close proximity to a parasite at 44hpi. (D) Number of CLEC4F+ cells normalized by area in Ring1, Ring2, bystander (By) tissue, and uninfected (UI) tissue at 44hpi. 3-6 matched ROI sets were counted from each of three mice. (E) Number of CLEC4F+ cells normalized by area in Ring1 and Ring2 at 24hpi. (F) Violin plot showing distribution of CLEC4F+ cells binned by distance from parasite edge at 24 and 44hpi. (G) Levels of CLEC4F+ cells in Ring1 and Ring2 around wild type (WT) and Spect2-/- parasites at 44hpi. ns, not significant.