Fig 1: The ability of Nef to impair autophagy initiation is genetically separable from other functional roles of the protein. A, B HEK293T cells were co-transfected with SERINC5-HA and either an empty vector, NL4-3 nef or 48–49 NL4-3 nef. 48 h later cells were analyzed by flow cytometry to measure the surface levels of MHC-I (A), and SERINC5 relative to the empty vector control (B). C HeLa TZM-bl cells were transfected with an empty vector, NL4-3 nef or 48–49 NL4-3 nef. 48 h later, cells were analyzed by flow cytometry for their surface levels of CD4 relative to the empty vector control. Data correspond to the mean and SEM of three biological replicates. Histograms are representative of three independent experiments. Significantly different values are indicated by asterisks **P = 0.01; ***P = 0.001; ****P = 0.0001
Fig 2: SERINC5 mAbs to the extracellular loops capture subtype B HIV-1(WR27). An equal amount of HIV-1WR27 (normalized by p24 core protein) was added to 96-well capture plates pre-coated with te indicated mAbs, using anti-luciferase (anti-Luc) as a negative control. The amount of virus bound by each antibody is indicated by the amount of viral p24 core protein (pg/mL) eluted from the antibodies after capture.
Supplier Page from MilliporeSigma for Anti-SERINC5 antibody produced in rabbit