Fig 1: Molecular expression patterns of lineage markers in healthy tissue, organoids and cervical cancers.a,b, Left: tiled images of cervix tissue sections from human (a) and mouse (b), including stratified and columnar epithelium and the transition zone, immunolabelled for KRT5 and KRT7; nuclei in blue. Boxed areas are magnified on the right. Images are representative of biologically independent experiments from three mice or human samples. c, Confocal images of human ectocervical stratified and endocervical columnar organoids immunolabelled for KRT5 and KRT7; nuclei in blue. Images representative of n = 3 donors. d, Labelling for bimodally-expressed proteins in the normal cervix, SCC and ADC. Tissue sections from normal tissue, SCC and ADC of the cervix were stained with haematoxylin and eosin or labelled with antibodies against KRT5, KRT7, KRT8, AGR2, GDA, MUC5B and CSTA; nuclei in blue. Magnifications of the boxed areas are shown in insets. Data representative of biologically independent experiments from five humans. e, Model depicting the two epithelial lineages and Wnt–Notch microenvironment in the transition zone and during squamous metaplasia.
Fig 2: Expression of KRT7 and lineage-specific markers.a, Tiled brightfield images of sections showing the entire mouse genital system labelled with smRNA-ISH for Krt7. Boxes are magnified on the right. Data representative of n = biologically independent experiments from 3 mice. b-d, Confocal images of human ectocervical stratified and endocervical columnar organoids immunolabeled for AGR2, GDA, KRT18, KRT5 and CSTA; nuclei in blue. Images are representative of biologically independent experiments from 3 humans.
Fig 3: CSTA expression was decreased in ESCC compared with normal esophageal mucosa. (A) CSTA transcript levels determined by RT-PCR in paired samples from 28 patients with ESCC. Data indicate expression relative to the mean levels of normal tissues. (B) Immunohistochemical analysis of CSTA expression in noncancerous tissue and ESCC specimens. Representative staining of CSTA-negative (=20% of the tumor cells are CSTA-positive) or CSTA-positive (>20% of the tumor cells are CSTA-positive) samples.
Fig 4: High CSTA expression in proliferative ESCC and lymph node metastases. (A) Representative images produced from formalin-fixed, paraffin-embedded samples of ESCC and adjacent noncancerous, normal mucosa, which were double-stained with anti-CSTA (dark brown) and anti-Ki67 (pink) antibodies. (B) Immunohistochemical analysis of CSTA expression in lymph node metastases of ESCC.
Supplier Page from MilliporeSigma for Anti-CSTA antibody produced in rabbit