Fig 1: CALR expressions in CD24+/CD44+/ESA+ cells. Cells were stained simultaneously with PE-Vio770–conjugated anti-CD44 antibody, FITC-conjugated anti-CD24 antibody, Pacific Blue–conjugated anti-ESA antibody, and Alexa Fluor 647–conjugated anti-CALR antibody and then sorted using a flow cytometer. Most cells were positive for CD44 among PANC-1 cells (A) and PANC-1-Lm cells (C). Positive cells for CD44 in PANC-1 cells were divided into 4 populations based on the CD24 and ESA expressions (B and D). High expression of CD44, CD24, or ESA was determined with the cutoff line of the top 1% of cells treated with each isotype control. The expression of CALR in each group was examined (B1–B4 and D1–D4). Gray histograms and dotted lines represent cells stained with anti-target and isotype control antibodies, respectively.