Fig 1: Loss of M1 mAChR protein in human post-mortem mod-Alzheimer’s disease temporal cortex. (A) mAChR1 protein levels are significantly reduced in the Mod-AD group (N = 7) compared to the control group (N = 7). (B) GluA2 protein levels are also significantly reduced in the Mod-AD group (N = 7) compared to the control group (N = 7). (C) GluN1 protein levels are not significantly different between the Mod-AD group (N = 7) compared to the control group (N = 7). (D) mGluR1 protein levels are not significantly different between the Mod-AD group (N = 7) and the control group (N = 7). (E) mGluR5 protein levels are significantly reduced in the Mod-AD group (N = 7) compared with the control group (N = 7). (F) Postsynaptic density-95 protein levels are not significantly different between the Mod-AD group (N = 7) and the control group (N = 7). Dots represent the value for each subject, and error bars represent the SEM. Significance level (*P < 0.05) was determined using an unpaired two-tailed Student’s t-test. Insets show cropped representative immunoblots, full length blots available in Supplementary Fig. 1. A.U. = arbitrary units.