FITC anti-mouse CD4 100509 from BioLegend

Product Specs
ItemFITC anti-mouse CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100509
NCBI Gene AliasesL3T4, Ly-4
Conjugate/TagFITC
CloneRM4-5
ApplicationsFlow Cytometry (FC)
ReactivityMouse
HostRat
IsotypeIgG2a, Kappa
Quantity50 µg
Antibody TypePrimary
NCBI Full Gene NameCD4 antigen
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenBALB/c mouse thymocytes
ClonalityMonoclonal
TargetCD4

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Strong immune responses and protection of PcrV and OprF-I mRNA vaccine candidates against Pseudomonas aeruginosaNPJ vaccinesMay 25, 2023Wang X, Liu C, Rcheulishvili N, Papukashvili D, Xie F, Zhao J, Hu X, Yu K, Yang N, Pan X, et al.(dilution 1:50) (100248, Biolegend, San Diego, California, US), antimouse FITC-CD4 (dilution 1:50) (100510, Biolegend, San Diego, California, US), and anti-mouse BV510-CD8 (dilution 1:100) (100752, Biolegend,

(2) Characterization of immune cell populations in syngeneic murine tumor modelsCancer MedicineMay 1, 2023Sofie Snipstad, Frida Bremnes, Mats Dehli Haugum, Einar Sulheim,NK cells,1:50 A,"Anti‐ CD3 (eBiosciences, 17‐0031‐82; Clone 145‐2C11)",T‐cells,1:62.5 A,"Anti‐CD4 (BioLegend, 100510; Clone RM4‐5)",CD4+ T cells,1:75 A,"Anti‐CD8a (eBiosciences, 17‐0031‐82; Clone 145‐2C11)",CD8+ T cells,1:250 B,"Anti‐F4/80

(3) American ginseng with different processing methods ameliorate immunosuppression induced by cyclophosphamide in mice via the MAPK signaling pathwaysFrontiers in ImmunologyApril 21, 2023Yan-Ting Zhang, Wei Tian, Yu-Shun Lu, Zhi-Man Li, Duo-Duo Ren, Yue Zhang, Ji-Yue Sha, Xiao-Hui Huo, Shan-Shan Li, Yin-Shi SunTang Pharmaceutical Co. Ltd. (Linyi, China). PE/Cyanine7 anti-mouse CD3 (100220), FITC anti-mouse CD4 (100509), and APC anti-mouse CD8a (100712) were obtained from BioLegend (San Diego, CA, USA). ConA (C8110) was

(4) Nanosecond Pulsed Electric Field Induces an Antitumor Effect in Triple-Negative Breast Cancer via CXCL9 Axis Dependence in MiceCancersMarch 30, 2023Xu Z, Pan C, Chen L, Qian J, Chen X, Zhou L, Zheng S.purchased for Zombie NIR Fixable Viability Kit (423106), CD45 (103112, 103140), CD3 (100218), CD4 (100510), CD8 (100706), CD11b (101245), F4/80 (123108) and CXCL9 (515606). The tumor was digested and ground.

(5) PCSK9 regulates the efficacy of immune checkpoint therapy in lung cancerFrontiers in immunologyJanuary 1, 2023Gao X, Yi L, Jiang C, Li S, Wang X, Yang B, Li W, Che N, Wang J, Zhang H, et al.(103188, BioLegend, CA, USA), PerCP/Cyanine5.5-anti-CD3ϵ (100328, BioLegend, CA, USA), FITC-anti-CD4 (100509, BioLegend, CA, USA), APC/FireTN750-anti-CD8a (100766, BioLegend, CA, USA), and BV421-anti-CD137 (740033,

(6) CEACAM1 is a direct SOX10 target and inhibits melanoma immune infiltration and stemnessiScienceDecember 22, 2022John Abou-Hamad, Jonathan J. Hodgins, Christiano T. de Souza, Brennan Garland, Cédrik Labrèche, Marie Marotel, Cameron Gibson, Samuel Delisle, Julia Pascoal, Rebecca C. Auer, et al.RRID:AB_493714 CD3 PeCy5,BioLegend,Cat# 100310; Clone 145-2C11 RRID:AB_312675 CD4 Fitc,BioLegend,Cat# 100510; Clone RM4-5 RRID:AB_312713 CD8 BV650,BioLegend,Cat# 100742; Clone 53-6.7; RRID:AB_2563056 CD8 BV786,BioLegend,Cat#

(7) p-Hydroxybenzoic Acid Ameliorates Colitis by Improving the Mucosal Barrier in a Gut Microbiota-Dependent MannerNutrientsDecember 18, 2022Han X, Li M, Sun L, Liu X, Yin Y, Hao J, Zhang W.CA, USA), PE/cy5 anti-mouse CD45 (103109, Biolegend, San Diego, CA, USA), and FITC anti-mouse CD4 (100509, Biolegend, San Diego, CA, USA) for 40 min at 4 °C. Then, cells were permeabilized with Fix/Perm buffer

(8) PRC2-Mediated Epigenetic Suppression of Type I IFN-STAT2 Signaling Impairs Antitumor Immunity in Luminal Breast CancerCancer researchDecember 16, 2022Hong J, Lee JH, Zhang Z, Wu Y, Yang M, Liao Y, de la Rosa R, Scheirer J, Pechacek D, Zhang N, et al.was performed with the indicated antibodies [anti-CD45 (BioLegend, #103116), anti-CD4 (BioLegend, #100510), anti-CD8 (BioLegend, #100708), and anti-CD3 (BioLegend, #100236)] for 20–30 minutes on ice. Antibodies

(9) HDAC5 Loss Enhances Phospholipid-Derived Arachidonic Acid Generation and Confers Sensitivity to cPLA2 Inhibition in Pancreatic CancerCancer ResearchDecember 16, 2022Penglin Pan, Gengdu Qin, Bo Wang, Haixin Yu, Jie Chen, Jiaying Liu, Kaijian Bing, Jian Shen, Dianyun Ren, Yuhan Zhao, et al.antibodies: APC-conjugated CD3 antibody (BioLegend, 100236); FITC-conjugated CD4 antibody (BioLegend, 100510); PerCP-conjugated CD45 antibody (BioLegend, 103130); PE-conjugated CD8 antibody (BioLegend, 100708).

(10) Coxsackievirus B infections are common in Cystic Fibrosis and experimental evidence supports protection by vaccinationiScienceOctober 21, 2022Virginia M. Stone, Renata Utorova, Marta Butrym, Amir-Babak Sioofy-Khojine, Minna M. Hankaniemi, Emma E. Ringqvist, Marfa Blanter, Anirudra Parajuli, Terezia Pincikova, Björn Fischler, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, FITC anti-mouse CD4,Biolegend,Clone RM4-5; Cat#100510; RRID:AB_312713 PE anti-mouse TCR beta chain,Biolegend,Clone H57-597; Cat#109207; RRID:AB_313430 APC anti-mouse/human CD45R/B220

(11) PD-L1 induction via the MEK-JNK-AP1 axis by a neddylation inhibitor promotes cancer-associated immunosuppressionCell death & diseaseOctober 3, 2022Zhang S, You X, Xu T, Chen Q, Li H, Dou L, Sun Y, Xiong X, Meredith MA, Sun Y.The permeabilized cells were then co-stained with antibodies against CD3 (100236, Biolegend), CD4 (100510, Biolegend) and CD8 (100708, Biolegend) or co-stained with antibodies against CD3, granzyme B (515403,

(12) CUL3/SPOP complex prevents immune escape and enhances chemotherapy sensitivity of ovarian cancer cells through degradation of PD-L1 proteinJournal for immunotherapy of cancerOctober 1, 2022Dong M, Qian M, Ruan Z.intracellular staining rupture buffer (421002, BioLegend). Afterwards, the cells were stained with CD4 (1/100, 100510, BioLegend), CD8 (1/100, 100708, BioLegend), anti-IFN-γ (1/100, 505808, BioLegend), anti-IL-2 (1/100,

(13) Interplay Between Liver Type 1 Innate Lymphoid Cells and NK Cells Drives the Development of Alcoholic SteatohepatitisCellular and Molecular Gastroenterology and HepatologySeptember 27, 2022Chen Cheng, Qian Zhang, Yue Li, Jiali Jiang, Linxi Xie, Haiyuan Shen, Dongqing Wu, Hejiao Zhang, Huiru Zhang, Xuan Wang, et al.NK1.1,156525,Biolegend PE anti-mouse CD49b,103506,Biolegend APC anti-mouse CD8,100712,Biolegend FITC anti-mouse CD4,100509,Biolegend APC anti-mouse CD19,115512,Biolegend APC anti-mouse CD49a,142606,Biolegend APC anti-mouse

(14) Immunogenicity and protective efficacy of a DNA vaccine inducing optimal expression of the SARS-CoV-2 S gene in hACE2 miceArchives of VirologySeptember 9, 2022Zhuo-xin Li et al.antibody, 0.2 mg/mL, lot: 100320, Biolegend), 0.5 μL of CD4 (FITC anti-mouse CD4 antibody, 0.5 mg/mL, lot: 100510, Biolegend), and 1.25 μL of CD8 (APC anti-mouse CD8 antibody, 0.2 mg/mL, lot: 100712, Biolegend) antibodies.

(15) Leishmania donovani Impedes Antileishmanial Immunity by Suppressing Dendritic Cells via the TIM-3 ReceptormBioAugust 30, 2022Akhtar MN, Kumar S, Sen P.allophycocyanin (APC)-labeled anti-CD11c (117310), APC-labeled anti-CD3 (100236), FITC-labeled anti-CD4 (100510), peridinin chlorophyll protein (PerCP)/Cyanin5.5-labeled anti-CD4 (100434), FITC-labeled anti-CD8 (100706),

(16) A highly selective JAK3 inhibitor is developed for treating rheumatoid arthritis by suppressing γc cytokine–related JAK-STAT signalScience AdvancesAugust 19, 2022Chengjuan Chen, Yuan Yin, Gaona Shi, Yu Zhou, Shuai Shao, Yazi Wei, Lei Wu, Dayong Zhang, Lan Sun, Tiantai Zhangbrefeldin A (BioLegend) for 2 hours, and then stained with an anti-CD4 antibody (1:100; BioLegend, 100510). After surface staining, cells were permeabilized with the Foxp3/Transcription Factor Staining Buffer

(17) Lkb1 aggravates diffuse large B-cell lymphoma by promoting the function of Treg cells and immune escapeJournal of Translational MedicineAugust 19, 2022Xiuhua Su, Tao Sun, Meng Li, Yuan Xia, Mingying Li, Dongmei Wang, Fei Lu, Jingjing Ye, Chunyan Ji328607), CD62L (Biolegend, 104411), and anti-mouse CD25 (Biolegend, 102007), anti-mouse CD4 (Biolegend, 100509). As CD127 expression inversely correlates with Foxp3 levels in CD25+Treg cells, the gate of CD127lo

(18) Angiotensin AT2 Receptor Stimulation Alleviates Collagen-Induced Arthritis by Upregulation of Regulatory T Cell NumbersFrontiers in ImmunologyJuly 8, 2022Bettina Sehnert, Veronica Valero-Esquitino, Georg Schett, Thomas Unger, Ulrike Muscha Steckelings, Reinhard Edmund VollGermany). CD4, CD8 and CD25 surface molecules were stained with FITC-conjugated anti-mouse CD4 (Biolegend #100509), PeCy5-conjugated anti-mouse CD8 (Biolegend # 100709) and PE-conjugated anti-mouse CD25 (Biolegend

(19) Proinflammatory signaling in islet β cells propagates invasion of pathogenic immune cells in autoimmune diabetesCell reportsJune 28, 2022Piñeros AR, Kulkarni A, Gao H, Orr KS, Glenn L, Huang F, Liu Y, Gannon M, Syed F, Wu W, et al.104718: RRID:AB_492824 anti-CD86,Biolegend,Cat# 105008: RRID:AB_313151 anti-CD4-FITC,Biolegend,Cat# 100510: RRID:AB_312713 anti-CD8,Biolegend,"Cat# 100734,:RRID:AB_2075238" anti-IL17A-AlexaFluor647,BD,Cat#

(20) Investigation of immune response induction by Japanese encephalitis live‐attenuated and chimeric vaccines in miceMedComm (2020)June 1, 2022Enyue Fang, Xinyu Liu, Xiaohui Liu, Ming Li, Ling Wang, Miao Li, Zelun Zhang, Yuhua Li, Yongxin YuFITC‐conjugated anti‐mouse CD4, and PerCP/Cy5.5‐conjugated anti‐mouse CD8a antibodies (BioLegend, Cat# 100206, 100510, 100734), followed by 20‐min incubation in the dark. Intracellular cytokines were detected after 30‐min

Write a Review

(1)

12/30/2019A Good CD4 Antibody from BioLegend Shikha SainiOverall, its a great antibody for flow cytometry.
(1)

10/22/2019CD4-FITC for Flow Cytometry of Mouse Splenocytes Kabir KhanThis is a great antibody to include in a panel when looking for CD4+ cells. As the CD4 molecule is highly expressed, a dimmer fluorophore like FITC is good to use.
(1)

1/29/2019Staining Mouse Splenocytes with Anti-CD4 FITC Adam GuessThis antibody was used to stain CD4+ cells in mouse splenocytes. It was chosen because it is a commonly used clone for CD4, FITC has the appropriate brightness index for use with CD4 and works well ... Read Review

FITC anti-mouse CD4 from BioLegend

Description

BioLegend

Citations (132)

Reviews

FITC anti-mouse CD4 from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map